Experiment: R2A with D-Cycloserine 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS30475 and RR42_RS30480 are separated by 241 nucleotides RR42_RS30480 and RR42_RS30485 are separated by 236 nucleotides RR42_RS30485 and RR42_RS30490 are separated by 43 nucleotides
RR42_RS30475: RR42_RS30475 - peptide transporter, at 2,210,879 to 2,212,729
_RS30475
RR42_RS30480: RR42_RS30480 - LysR family transcriptional regulator, at 2,212,971 to 2,213,936
_RS30480
RR42_RS30485: RR42_RS30485 - hypothetical protein, at 2,214,173 to 2,214,526
_RS30485
RR42_RS30490: RR42_RS30490 - hypothetical protein, at 2,214,570 to 2,214,902
_RS30490
Position (kb)
2212
2213
2214 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2212.279 kb on - strand, within RR42_RS30475 at 2212.279 kb on - strand, within RR42_RS30475 at 2212.323 kb on + strand, within RR42_RS30475 at 2212.323 kb on + strand, within RR42_RS30475 at 2212.323 kb on + strand, within RR42_RS30475 at 2212.323 kb on + strand, within RR42_RS30475 at 2212.323 kb on + strand, within RR42_RS30475 at 2212.324 kb on - strand, within RR42_RS30475 at 2212.324 kb on - strand, within RR42_RS30475 at 2212.432 kb on + strand, within RR42_RS30475 at 2212.434 kb on + strand, within RR42_RS30475 at 2212.434 kb on + strand, within RR42_RS30475 at 2212.542 kb on + strand, within RR42_RS30475 at 2212.542 kb on + strand, within RR42_RS30475 at 2212.543 kb on - strand, within RR42_RS30475 at 2212.609 kb on - strand at 2212.696 kb on + strand at 2212.697 kb on - strand at 2212.697 kb on - strand at 2212.848 kb on + strand at 2212.894 kb on + strand at 2212.895 kb on - strand at 2213.052 kb on - strand at 2213.110 kb on + strand, within RR42_RS30480 at 2213.110 kb on + strand, within RR42_RS30480 at 2213.110 kb on + strand, within RR42_RS30480 at 2213.110 kb on + strand, within RR42_RS30480 at 2213.161 kb on - strand, within RR42_RS30480 at 2213.244 kb on + strand, within RR42_RS30480 at 2213.245 kb on - strand, within RR42_RS30480 at 2213.245 kb on - strand, within RR42_RS30480 at 2213.416 kb on + strand, within RR42_RS30480 at 2213.417 kb on - strand, within RR42_RS30480 at 2213.545 kb on + strand, within RR42_RS30480 at 2213.545 kb on + strand, within RR42_RS30480 at 2213.546 kb on - strand, within RR42_RS30480 at 2213.546 kb on - strand, within RR42_RS30480 at 2213.546 kb on - strand, within RR42_RS30480 at 2213.622 kb on + strand, within RR42_RS30480 at 2213.622 kb on + strand, within RR42_RS30480 at 2213.622 kb on + strand, within RR42_RS30480 at 2213.623 kb on - strand, within RR42_RS30480 at 2213.905 kb on + strand at 2213.905 kb on + strand at 2213.907 kb on + strand at 2213.907 kb on + strand at 2213.907 kb on + strand at 2213.972 kb on + strand at 2213.978 kb on - strand at 2214.004 kb on + strand at 2214.036 kb on + strand at 2214.037 kb on - strand at 2214.219 kb on + strand, within RR42_RS30485 at 2214.220 kb on - strand, within RR42_RS30485 at 2214.221 kb on + strand, within RR42_RS30485 at 2214.221 kb on + strand, within RR42_RS30485 at 2214.222 kb on - strand, within RR42_RS30485 at 2214.284 kb on + strand, within RR42_RS30485 at 2214.284 kb on + strand, within RR42_RS30485 at 2214.381 kb on - strand, within RR42_RS30485 at 2214.408 kb on + strand, within RR42_RS30485 at 2214.409 kb on - strand, within RR42_RS30485 at 2214.409 kb on - strand, within RR42_RS30485 at 2214.494 kb on + strand at 2214.494 kb on + strand at 2214.495 kb on - strand at 2214.517 kb on - strand at 2214.517 kb on - strand at 2214.546 kb on + strand at 2214.547 kb on - strand at 2214.766 kb on - strand, within RR42_RS30490 at 2214.900 kb on + strand at 2214.901 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.01 mg/ml remove 2,212,279 - RR42_RS30475 0.76 +0.7 2,212,279 - RR42_RS30475 0.76 +1.0 2,212,323 + RR42_RS30475 0.78 -0.5 2,212,323 + RR42_RS30475 0.78 +0.3 2,212,323 + RR42_RS30475 0.78 -0.1 2,212,323 + RR42_RS30475 0.78 -0.9 2,212,323 + RR42_RS30475 0.78 -0.4 2,212,324 - RR42_RS30475 0.78 -0.8 2,212,324 - RR42_RS30475 0.78 +0.3 2,212,432 + RR42_RS30475 0.84 -0.2 2,212,434 + RR42_RS30475 0.84 +0.6 2,212,434 + RR42_RS30475 0.84 -0.7 2,212,542 + RR42_RS30475 0.90 +0.3 2,212,542 + RR42_RS30475 0.90 -0.4 2,212,543 - RR42_RS30475 0.90 -0.2 2,212,609 - -1.5 2,212,696 + -0.5 2,212,697 - -1.0 2,212,697 - -1.2 2,212,848 + -0.2 2,212,894 + +0.4 2,212,895 - -0.3 2,213,052 - +0.4 2,213,110 + RR42_RS30480 0.14 +0.4 2,213,110 + RR42_RS30480 0.14 +0.8 2,213,110 + RR42_RS30480 0.14 -0.2 2,213,110 + RR42_RS30480 0.14 +0.9 2,213,161 - RR42_RS30480 0.20 +1.7 2,213,244 + RR42_RS30480 0.28 +1.2 2,213,245 - RR42_RS30480 0.28 +0.8 2,213,245 - RR42_RS30480 0.28 -0.4 2,213,416 + RR42_RS30480 0.46 +0.4 2,213,417 - RR42_RS30480 0.46 +0.2 2,213,545 + RR42_RS30480 0.59 -0.4 2,213,545 + RR42_RS30480 0.59 -0.4 2,213,546 - RR42_RS30480 0.60 +0.5 2,213,546 - RR42_RS30480 0.60 +0.2 2,213,546 - RR42_RS30480 0.60 -0.6 2,213,622 + RR42_RS30480 0.67 -1.2 2,213,622 + RR42_RS30480 0.67 +0.2 2,213,622 + RR42_RS30480 0.67 +0.2 2,213,623 - RR42_RS30480 0.67 +0.5 2,213,905 + -0.1 2,213,905 + +0.8 2,213,907 + +0.6 2,213,907 + -0.2 2,213,907 + -1.2 2,213,972 + +0.6 2,213,978 - -0.4 2,214,004 + -1.3 2,214,036 + -0.5 2,214,037 - -0.9 2,214,219 + RR42_RS30485 0.13 -0.9 2,214,220 - RR42_RS30485 0.13 -1.2 2,214,221 + RR42_RS30485 0.14 -0.6 2,214,221 + RR42_RS30485 0.14 +0.0 2,214,222 - RR42_RS30485 0.14 +0.3 2,214,284 + RR42_RS30485 0.31 -0.4 2,214,284 + RR42_RS30485 0.31 -1.3 2,214,381 - RR42_RS30485 0.59 +0.4 2,214,408 + RR42_RS30485 0.66 -2.8 2,214,409 - RR42_RS30485 0.67 -0.7 2,214,409 - RR42_RS30485 0.67 -1.7 2,214,494 + -2.8 2,214,494 + -1.9 2,214,495 - +1.8 2,214,517 - +0.2 2,214,517 - -0.5 2,214,546 + -0.2 2,214,547 - -0.3 2,214,766 - RR42_RS30490 0.59 -1.3 2,214,900 + +0.4 2,214,901 - -1.2
Or see this region's nucleotide sequence