Experiment: R2A with D-Cycloserine 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS29225 and RR42_RS29230 are separated by 188 nucleotides RR42_RS29230 and RR42_RS29235 are separated by 105 nucleotides RR42_RS29235 and RR42_RS29240 are separated by 379 nucleotides
RR42_RS29225: RR42_RS29225 - NADH-ubiquinone oxidoreductase subunit 6, at 1,912,091 to 1,913,413
_RS29225
RR42_RS29230: RR42_RS29230 - TetR family transcriptional regulator, at 1,913,602 to 1,914,216
_RS29230
RR42_RS29235: RR42_RS29235 - hypothetical protein, at 1,914,322 to 1,914,591
_RS29235
RR42_RS29240: RR42_RS29240 - nitrile hydratase subunit alpha, at 1,914,971 to 1,915,675
_RS29240
Position (kb) 1913
1914
1915 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 1912.740 kb on - strand, within RR42_RS29225 at 1912.743 kb on + strand, within RR42_RS29225 at 1912.743 kb on + strand, within RR42_RS29225 at 1912.743 kb on + strand, within RR42_RS29225 at 1912.744 kb on - strand, within RR42_RS29225 at 1912.744 kb on - strand, within RR42_RS29225 at 1912.744 kb on - strand, within RR42_RS29225 at 1912.899 kb on + strand, within RR42_RS29225 at 1912.899 kb on + strand, within RR42_RS29225 at 1912.899 kb on + strand, within RR42_RS29225 at 1912.899 kb on + strand, within RR42_RS29225 at 1912.900 kb on - strand, within RR42_RS29225 at 1912.900 kb on - strand, within RR42_RS29225 at 1912.924 kb on - strand, within RR42_RS29225 at 1912.924 kb on - strand, within RR42_RS29225 at 1913.134 kb on + strand, within RR42_RS29225 at 1913.134 kb on + strand, within RR42_RS29225 at 1913.134 kb on + strand, within RR42_RS29225 at 1913.134 kb on + strand, within RR42_RS29225 at 1913.134 kb on + strand, within RR42_RS29225 at 1913.135 kb on - strand, within RR42_RS29225 at 1913.135 kb on - strand, within RR42_RS29225 at 1913.135 kb on - strand, within RR42_RS29225 at 1913.145 kb on + strand, within RR42_RS29225 at 1913.190 kb on + strand, within RR42_RS29225 at 1913.190 kb on + strand, within RR42_RS29225 at 1913.190 kb on + strand, within RR42_RS29225 at 1913.269 kb on + strand, within RR42_RS29225 at 1913.269 kb on + strand, within RR42_RS29225 at 1913.270 kb on - strand, within RR42_RS29225 at 1913.270 kb on - strand, within RR42_RS29225 at 1913.270 kb on - strand, within RR42_RS29225 at 1913.270 kb on - strand, within RR42_RS29225 at 1913.283 kb on + strand at 1913.283 kb on + strand at 1913.284 kb on - strand at 1913.284 kb on - strand at 1913.284 kb on - strand at 1913.284 kb on - strand at 1913.284 kb on - strand at 1913.327 kb on + strand at 1913.327 kb on + strand at 1913.331 kb on + strand at 1913.332 kb on - strand at 1913.332 kb on - strand at 1913.366 kb on + strand at 1913.392 kb on - strand at 1913.544 kb on + strand at 1913.544 kb on + strand at 1913.544 kb on + strand at 1913.544 kb on + strand at 1913.545 kb on - strand at 1913.545 kb on - strand at 1913.545 kb on - strand at 1913.762 kb on - strand, within RR42_RS29230 at 1913.983 kb on + strand, within RR42_RS29230 at 1913.983 kb on + strand, within RR42_RS29230 at 1913.984 kb on - strand, within RR42_RS29230 at 1914.094 kb on + strand, within RR42_RS29230 at 1914.094 kb on + strand, within RR42_RS29230 at 1914.094 kb on + strand, within RR42_RS29230 at 1914.095 kb on - strand, within RR42_RS29230 at 1914.095 kb on - strand, within RR42_RS29230 at 1914.095 kb on - strand, within RR42_RS29230 at 1914.095 kb on - strand, within RR42_RS29230 at 1914.095 kb on - strand, within RR42_RS29230 at 1914.097 kb on + strand, within RR42_RS29230 at 1914.127 kb on + strand, within RR42_RS29230 at 1914.139 kb on + strand, within RR42_RS29230 at 1914.139 kb on + strand, within RR42_RS29230 at 1914.139 kb on + strand, within RR42_RS29230 at 1914.139 kb on + strand, within RR42_RS29230 at 1914.139 kb on + strand, within RR42_RS29230 at 1914.140 kb on - strand, within RR42_RS29230 at 1914.140 kb on - strand, within RR42_RS29230 at 1914.140 kb on - strand, within RR42_RS29230 at 1914.204 kb on + strand at 1914.205 kb on - strand at 1914.205 kb on - strand at 1914.205 kb on - strand at 1914.214 kb on + strand at 1914.256 kb on + strand at 1914.256 kb on + strand at 1914.256 kb on + strand at 1914.256 kb on + strand at 1914.256 kb on + strand at 1914.256 kb on + strand at 1914.256 kb on + strand at 1914.256 kb on + strand at 1914.256 kb on + strand at 1914.256 kb on + strand at 1914.257 kb on - strand at 1914.257 kb on - strand at 1914.257 kb on - strand at 1914.257 kb on - strand at 1914.257 kb on - strand at 1914.257 kb on - strand at 1914.257 kb on - strand at 1914.257 kb on - strand at 1914.262 kb on + strand at 1914.262 kb on + strand at 1914.262 kb on + strand at 1914.262 kb on + strand at 1914.262 kb on + strand at 1914.262 kb on + strand at 1914.262 kb on + strand at 1914.263 kb on - strand at 1914.263 kb on - strand at 1914.263 kb on - strand at 1914.263 kb on - strand at 1914.338 kb on + strand at 1914.338 kb on + strand at 1914.338 kb on + strand at 1914.339 kb on - strand at 1914.339 kb on - strand at 1914.339 kb on - strand at 1914.503 kb on + strand, within RR42_RS29235 at 1914.504 kb on - strand, within RR42_RS29235 at 1914.521 kb on + strand, within RR42_RS29235 at 1914.521 kb on + strand, within RR42_RS29235 at 1914.522 kb on - strand, within RR42_RS29235 at 1914.522 kb on - strand, within RR42_RS29235 at 1914.545 kb on - strand, within RR42_RS29235 at 1914.572 kb on + strand at 1914.572 kb on + strand at 1914.572 kb on + strand at 1914.573 kb on - strand at 1914.573 kb on - strand at 1914.675 kb on + strand at 1914.675 kb on + strand at 1914.675 kb on + strand at 1914.676 kb on - strand at 1914.676 kb on - strand at 1914.676 kb on - strand at 1914.763 kb on + strand at 1914.763 kb on + strand at 1914.764 kb on - strand at 1914.764 kb on - strand at 1914.764 kb on - strand at 1914.912 kb on - strand at 1915.130 kb on + strand, within RR42_RS29240 at 1915.169 kb on + strand, within RR42_RS29240 at 1915.169 kb on + strand, within RR42_RS29240 at 1915.169 kb on + strand, within RR42_RS29240 at 1915.169 kb on + strand, within RR42_RS29240 at 1915.169 kb on + strand, within RR42_RS29240 at 1915.170 kb on - strand, within RR42_RS29240 at 1915.170 kb on - strand, within RR42_RS29240 at 1915.170 kb on - strand, within RR42_RS29240
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.01 mg/ml remove 1,912,740 - RR42_RS29225 0.49 -0.1 1,912,743 + RR42_RS29225 0.49 +0.5 1,912,743 + RR42_RS29225 0.49 -0.9 1,912,743 + RR42_RS29225 0.49 +1.0 1,912,744 - RR42_RS29225 0.49 +0.4 1,912,744 - RR42_RS29225 0.49 -0.7 1,912,744 - RR42_RS29225 0.49 +0.0 1,912,899 + RR42_RS29225 0.61 -0.2 1,912,899 + RR42_RS29225 0.61 -1.3 1,912,899 + RR42_RS29225 0.61 -0.2 1,912,899 + RR42_RS29225 0.61 +0.0 1,912,900 - RR42_RS29225 0.61 +0.7 1,912,900 - RR42_RS29225 0.61 -0.4 1,912,924 - RR42_RS29225 0.63 +0.3 1,912,924 - RR42_RS29225 0.63 +1.3 1,913,134 + RR42_RS29225 0.79 -0.3 1,913,134 + RR42_RS29225 0.79 -0.2 1,913,134 + RR42_RS29225 0.79 -0.6 1,913,134 + RR42_RS29225 0.79 +0.3 1,913,134 + RR42_RS29225 0.79 -0.9 1,913,135 - RR42_RS29225 0.79 -0.0 1,913,135 - RR42_RS29225 0.79 -0.6 1,913,135 - RR42_RS29225 0.79 +0.6 1,913,145 + RR42_RS29225 0.80 +0.6 1,913,190 + RR42_RS29225 0.83 -0.2 1,913,190 + RR42_RS29225 0.83 +1.1 1,913,190 + RR42_RS29225 0.83 +0.1 1,913,269 + RR42_RS29225 0.89 -0.0 1,913,269 + RR42_RS29225 0.89 +1.8 1,913,270 - RR42_RS29225 0.89 -0.1 1,913,270 - RR42_RS29225 0.89 -0.3 1,913,270 - RR42_RS29225 0.89 +0.1 1,913,270 - RR42_RS29225 0.89 +1.4 1,913,283 + +0.3 1,913,283 + -0.0 1,913,284 - +0.1 1,913,284 - -2.2 1,913,284 - -1.0 1,913,284 - +1.3 1,913,284 - -0.1 1,913,327 + +0.6 1,913,327 + -0.7 1,913,331 + -0.1 1,913,332 - -0.4 1,913,332 - +0.6 1,913,366 + +0.7 1,913,392 - -0.0 1,913,544 + -1.8 1,913,544 + +0.6 1,913,544 + +0.8 1,913,544 + +0.5 1,913,545 - -0.5 1,913,545 - +0.5 1,913,545 - +0.4 1,913,762 - RR42_RS29230 0.26 +2.6 1,913,983 + RR42_RS29230 0.62 +0.4 1,913,983 + RR42_RS29230 0.62 +0.9 1,913,984 - RR42_RS29230 0.62 +0.5 1,914,094 + RR42_RS29230 0.80 -0.9 1,914,094 + RR42_RS29230 0.80 +1.1 1,914,094 + RR42_RS29230 0.80 +0.7 1,914,095 - RR42_RS29230 0.80 +0.4 1,914,095 - RR42_RS29230 0.80 +0.5 1,914,095 - RR42_RS29230 0.80 +0.3 1,914,095 - RR42_RS29230 0.80 +0.8 1,914,095 - RR42_RS29230 0.80 +1.5 1,914,097 + RR42_RS29230 0.80 +0.2 1,914,127 + RR42_RS29230 0.85 +0.2 1,914,139 + RR42_RS29230 0.87 -0.3 1,914,139 + RR42_RS29230 0.87 +1.0 1,914,139 + RR42_RS29230 0.87 +1.2 1,914,139 + RR42_RS29230 0.87 +0.3 1,914,139 + RR42_RS29230 0.87 +0.2 1,914,140 - RR42_RS29230 0.87 +0.4 1,914,140 - RR42_RS29230 0.87 -0.7 1,914,140 - RR42_RS29230 0.87 -0.1 1,914,204 + -0.8 1,914,205 - +0.1 1,914,205 - -0.1 1,914,205 - -0.6 1,914,214 + -0.4 1,914,256 + -0.7 1,914,256 + +0.3 1,914,256 + -0.5 1,914,256 + -0.2 1,914,256 + +0.5 1,914,256 + -0.6 1,914,256 + -0.8 1,914,256 + -0.3 1,914,256 + -0.4 1,914,256 + +0.2 1,914,257 - -0.5 1,914,257 - -1.0 1,914,257 - +0.6 1,914,257 - -0.0 1,914,257 - -0.0 1,914,257 - +0.7 1,914,257 - +0.4 1,914,257 - -0.7 1,914,262 + -0.6 1,914,262 + -1.0 1,914,262 + +0.4 1,914,262 + +0.6 1,914,262 + +0.2 1,914,262 + +0.9 1,914,262 + -0.5 1,914,263 - -0.1 1,914,263 - +0.9 1,914,263 - +0.2 1,914,263 - -0.6 1,914,338 + +0.1 1,914,338 + +0.0 1,914,338 + -0.2 1,914,339 - -0.6 1,914,339 - +0.3 1,914,339 - +0.2 1,914,503 + RR42_RS29235 0.67 -0.9 1,914,504 - RR42_RS29235 0.67 -0.2 1,914,521 + RR42_RS29235 0.74 +0.0 1,914,521 + RR42_RS29235 0.74 -0.0 1,914,522 - RR42_RS29235 0.74 +1.1 1,914,522 - RR42_RS29235 0.74 -1.2 1,914,545 - RR42_RS29235 0.83 -2.9 1,914,572 + -1.2 1,914,572 + -0.8 1,914,572 + +0.2 1,914,573 - -1.2 1,914,573 - +0.3 1,914,675 + +1.5 1,914,675 + -0.8 1,914,675 + +1.2 1,914,676 - +0.3 1,914,676 - -0.5 1,914,676 - +0.0 1,914,763 + -0.4 1,914,763 + +0.2 1,914,764 - -0.7 1,914,764 - -1.6 1,914,764 - +0.1 1,914,912 - -0.1 1,915,130 + RR42_RS29240 0.23 -0.9 1,915,169 + RR42_RS29240 0.28 -0.3 1,915,169 + RR42_RS29240 0.28 -0.6 1,915,169 + RR42_RS29240 0.28 +0.6 1,915,169 + RR42_RS29240 0.28 +1.3 1,915,169 + RR42_RS29240 0.28 -0.8 1,915,170 - RR42_RS29240 0.28 +0.2 1,915,170 - RR42_RS29240 0.28 -0.6 1,915,170 - RR42_RS29240 0.28 +0.2
Or see this region's nucleotide sequence