Strain Fitness in Cupriavidus basilensis FW507-4G11 around RR42_RS26550

Experiment: R2A with D-Cycloserine 0.01 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRR42_RS26545 and RR42_RS26550 are separated by 3 nucleotidesRR42_RS26550 and RR42_RS26555 are separated by 235 nucleotidesRR42_RS26555 and RR42_RS26560 are separated by 269 nucleotidesRR42_RS26560 and RR42_RS26565 are separated by 166 nucleotides RR42_RS26545: RR42_RS26545 - ABC transporter substrate-binding protein, at 1,311,172 to 1,312,155 _RS26545 RR42_RS26550: RR42_RS26550 - gluconolactonase, at 1,312,159 to 1,313,103 _RS26550 RR42_RS26555: RR42_RS26555 - tRNA-Met, at 1,313,339 to 1,313,414 _RS26555 RR42_RS26560: RR42_RS26560 - hypothetical protein, at 1,313,684 to 1,313,923 _RS26560 RR42_RS26565: RR42_RS26565 - 5'-3' exonuclease, at 1,314,090 to 1,314,896 _RS26565 Position (kb) 1312 1313 1314Strain fitness (log2 ratio) -3 -2 -1 0 1at 1311.412 kb on - strand, within RR42_RS26545at 1311.472 kb on + strand, within RR42_RS26545at 1311.472 kb on + strand, within RR42_RS26545at 1311.514 kb on + strand, within RR42_RS26545at 1311.514 kb on + strand, within RR42_RS26545at 1311.515 kb on - strand, within RR42_RS26545at 1311.589 kb on + strand, within RR42_RS26545at 1311.646 kb on + strand, within RR42_RS26545at 1311.646 kb on + strand, within RR42_RS26545at 1311.647 kb on - strand, within RR42_RS26545at 1311.647 kb on - strand, within RR42_RS26545at 1311.647 kb on - strand, within RR42_RS26545at 1312.153 kb on + strandat 1312.153 kb on + strandat 1312.154 kb on - strandat 1312.154 kb on - strandat 1312.154 kb on - strandat 1312.324 kb on + strand, within RR42_RS26550at 1312.324 kb on + strand, within RR42_RS26550at 1312.325 kb on - strand, within RR42_RS26550at 1312.325 kb on - strand, within RR42_RS26550at 1312.325 kb on - strand, within RR42_RS26550at 1312.325 kb on - strand, within RR42_RS26550at 1312.325 kb on - strand, within RR42_RS26550at 1312.359 kb on + strand, within RR42_RS26550at 1312.360 kb on - strand, within RR42_RS26550at 1312.507 kb on + strand, within RR42_RS26550at 1312.579 kb on + strand, within RR42_RS26550at 1312.579 kb on + strand, within RR42_RS26550at 1312.579 kb on + strand, within RR42_RS26550at 1312.579 kb on + strand, within RR42_RS26550at 1312.580 kb on - strand, within RR42_RS26550at 1312.580 kb on - strand, within RR42_RS26550at 1312.618 kb on - strand, within RR42_RS26550at 1312.618 kb on - strand, within RR42_RS26550at 1312.630 kb on + strand, within RR42_RS26550at 1312.806 kb on + strand, within RR42_RS26550at 1312.806 kb on + strand, within RR42_RS26550at 1312.806 kb on + strand, within RR42_RS26550at 1312.814 kb on - strand, within RR42_RS26550at 1312.888 kb on + strand, within RR42_RS26550at 1312.888 kb on + strand, within RR42_RS26550at 1312.888 kb on + strand, within RR42_RS26550at 1312.889 kb on - strand, within RR42_RS26550at 1312.889 kb on - strand, within RR42_RS26550at 1313.321 kb on + strandat 1313.321 kb on + strandat 1313.378 kb on + strand, within RR42_RS26555at 1313.425 kb on + strandat 1313.448 kb on + strandat 1313.464 kb on + strandat 1313.465 kb on - strandat 1313.582 kb on + strandat 1313.582 kb on + strandat 1313.850 kb on + strand, within RR42_RS26560at 1313.850 kb on + strand, within RR42_RS26560at 1313.851 kb on - strand, within RR42_RS26560at 1313.851 kb on - strand, within RR42_RS26560at 1313.851 kb on - strand, within RR42_RS26560at 1313.891 kb on + strand, within RR42_RS26560at 1313.892 kb on - strand, within RR42_RS26560at 1313.933 kb on + strandat 1313.934 kb on - strandat 1313.934 kb on - strandat 1313.934 kb on - strandat 1314.041 kb on - strandat 1314.103 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.01 mg/ml
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1,311,412 - RR42_RS26545 0.24 +1.1
1,311,472 + RR42_RS26545 0.30 +0.3
1,311,472 + RR42_RS26545 0.30 -1.9
1,311,514 + RR42_RS26545 0.35 -0.0
1,311,514 + RR42_RS26545 0.35 -0.1
1,311,515 - RR42_RS26545 0.35 -0.5
1,311,589 + RR42_RS26545 0.42 +0.2
1,311,646 + RR42_RS26545 0.48 +0.6
1,311,646 + RR42_RS26545 0.48 -0.2
1,311,647 - RR42_RS26545 0.48 +0.1
1,311,647 - RR42_RS26545 0.48 +0.6
1,311,647 - RR42_RS26545 0.48 -0.1
1,312,153 + +0.3
1,312,153 + +0.3
1,312,154 - -0.1
1,312,154 - -0.8
1,312,154 - -0.0
1,312,324 + RR42_RS26550 0.17 -0.5
1,312,324 + RR42_RS26550 0.17 +0.1
1,312,325 - RR42_RS26550 0.18 +0.3
1,312,325 - RR42_RS26550 0.18 +0.8
1,312,325 - RR42_RS26550 0.18 -1.0
1,312,325 - RR42_RS26550 0.18 -1.0
1,312,325 - RR42_RS26550 0.18 -1.6
1,312,359 + RR42_RS26550 0.21 +0.3
1,312,360 - RR42_RS26550 0.21 -0.1
1,312,507 + RR42_RS26550 0.37 -0.1
1,312,579 + RR42_RS26550 0.44 +0.8
1,312,579 + RR42_RS26550 0.44 +1.0
1,312,579 + RR42_RS26550 0.44 -0.2
1,312,579 + RR42_RS26550 0.44 -0.4
1,312,580 - RR42_RS26550 0.45 +0.4
1,312,580 - RR42_RS26550 0.45 +0.3
1,312,618 - RR42_RS26550 0.49 +1.1
1,312,618 - RR42_RS26550 0.49 +0.6
1,312,630 + RR42_RS26550 0.50 +0.8
1,312,806 + RR42_RS26550 0.68 -0.0
1,312,806 + RR42_RS26550 0.68 -1.2
1,312,806 + RR42_RS26550 0.68 +0.3
1,312,814 - RR42_RS26550 0.69 +0.9
1,312,888 + RR42_RS26550 0.77 +1.0
1,312,888 + RR42_RS26550 0.77 +0.6
1,312,888 + RR42_RS26550 0.77 +0.6
1,312,889 - RR42_RS26550 0.77 +0.5
1,312,889 - RR42_RS26550 0.77 +0.2
1,313,321 + -0.2
1,313,321 + -0.2
1,313,378 + RR42_RS26555 0.51 -0.2
1,313,425 + +0.6
1,313,448 + +0.4
1,313,464 + -0.6
1,313,465 - +0.2
1,313,582 + -2.5
1,313,582 + +0.2
1,313,850 + RR42_RS26560 0.69 +0.8
1,313,850 + RR42_RS26560 0.69 +0.5
1,313,851 - RR42_RS26560 0.70 -2.8
1,313,851 - RR42_RS26560 0.70 -0.3
1,313,851 - RR42_RS26560 0.70 -1.7
1,313,891 + RR42_RS26560 0.86 -0.8
1,313,892 - RR42_RS26560 0.87 -0.0
1,313,933 + +0.2
1,313,934 - -0.0
1,313,934 - +1.0
1,313,934 - -0.7
1,314,041 - +0.3
1,314,103 + +0.8

Or see this region's nucleotide sequence