Experiment: R2A with D-Cycloserine 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS26475 and RR42_RS26480 are separated by 1 nucleotides RR42_RS26480 and RR42_RS26485 are separated by 39 nucleotides RR42_RS26485 and RR42_RS26490 overlap by 16 nucleotides RR42_RS26490 and RR42_RS26495 are separated by 251 nucleotides
RR42_RS26475: RR42_RS26475 - Asp/Glu racemase, at 1,295,852 to 1,296,604
_RS26475
RR42_RS26480: RR42_RS26480 - alpha/beta hydrolase, at 1,296,606 to 1,297,451
_RS26480
RR42_RS26485: RR42_RS26485 - 2,5-dihydroxypyridine 5,6-dioxygenase, at 1,297,491 to 1,298,537
_RS26485
RR42_RS26490: RR42_RS26490 - N-carbamoylsarcosine amidase, at 1,298,522 to 1,299,163
_RS26490
RR42_RS26495: RR42_RS26495 - carbon monoxide dehydrogenase, at 1,299,415 to 1,301,823
_RS26495
Position (kb)
1297
1298
1299 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1296.530 kb on + strand at 1296.635 kb on - strand at 1296.660 kb on + strand at 1296.661 kb on - strand at 1296.789 kb on + strand, within RR42_RS26480 at 1296.789 kb on + strand, within RR42_RS26480 at 1296.789 kb on + strand, within RR42_RS26480 at 1296.790 kb on - strand, within RR42_RS26480 at 1296.790 kb on - strand, within RR42_RS26480 at 1296.906 kb on + strand, within RR42_RS26480 at 1297.050 kb on + strand, within RR42_RS26480 at 1297.051 kb on - strand, within RR42_RS26480 at 1297.389 kb on + strand at 1297.390 kb on - strand at 1297.581 kb on - strand at 1297.581 kb on - strand at 1297.581 kb on - strand at 1297.714 kb on - strand, within RR42_RS26485 at 1297.714 kb on - strand, within RR42_RS26485 at 1298.022 kb on + strand, within RR42_RS26485 at 1298.242 kb on - strand, within RR42_RS26485 at 1298.334 kb on + strand, within RR42_RS26485 at 1298.374 kb on - strand, within RR42_RS26485 at 1299.256 kb on + strand at 1299.415 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.01 mg/ml remove 1,296,530 + +0.3 1,296,635 - +1.6 1,296,660 + +0.6 1,296,661 - -0.1 1,296,789 + RR42_RS26480 0.22 -0.8 1,296,789 + RR42_RS26480 0.22 -0.3 1,296,789 + RR42_RS26480 0.22 -0.8 1,296,790 - RR42_RS26480 0.22 -0.1 1,296,790 - RR42_RS26480 0.22 +0.4 1,296,906 + RR42_RS26480 0.35 -2.0 1,297,050 + RR42_RS26480 0.52 +0.3 1,297,051 - RR42_RS26480 0.53 -0.0 1,297,389 + +0.7 1,297,390 - -0.4 1,297,581 - -0.2 1,297,581 - -0.0 1,297,581 - +0.2 1,297,714 - RR42_RS26485 0.21 +0.4 1,297,714 - RR42_RS26485 0.21 +0.8 1,298,022 + RR42_RS26485 0.51 +0.3 1,298,242 - RR42_RS26485 0.72 +0.2 1,298,334 + RR42_RS26485 0.81 +1.0 1,298,374 - RR42_RS26485 0.84 +0.7 1,299,256 + +1.8 1,299,415 - -1.6
Or see this region's nucleotide sequence