Experiment: R2A with D-Cycloserine 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS25600 and RR42_RS25605 are separated by 85 nucleotides RR42_RS25605 and RR42_RS25610 are separated by 148 nucleotides
RR42_RS25600: RR42_RS25600 - AraC family transcriptional regulator, at 1,087,712 to 1,088,500
_RS25600
RR42_RS25605: RR42_RS25605 - N-ethylmaleimide reductase, at 1,088,586 to 1,089,680
_RS25605
RR42_RS25610: RR42_RS25610 - alpha/beta hydrolase, at 1,089,829 to 1,090,668
_RS25610
Position (kb)
1088
1089
1090 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1087.651 kb on + strand at 1087.652 kb on - strand at 1087.652 kb on - strand at 1087.693 kb on + strand at 1087.714 kb on - strand at 1088.016 kb on + strand, within RR42_RS25600 at 1088.043 kb on + strand, within RR42_RS25600 at 1088.043 kb on + strand, within RR42_RS25600 at 1088.443 kb on - strand at 1088.587 kb on + strand at 1088.587 kb on + strand at 1088.588 kb on - strand at 1088.626 kb on + strand at 1088.626 kb on + strand at 1088.627 kb on - strand at 1088.627 kb on - strand at 1088.792 kb on - strand, within RR42_RS25605 at 1088.809 kb on + strand, within RR42_RS25605 at 1088.809 kb on + strand, within RR42_RS25605 at 1088.809 kb on + strand, within RR42_RS25605 at 1088.810 kb on - strand, within RR42_RS25605 at 1088.810 kb on - strand, within RR42_RS25605 at 1088.899 kb on + strand, within RR42_RS25605 at 1088.935 kb on + strand, within RR42_RS25605 at 1089.457 kb on + strand, within RR42_RS25605 at 1089.457 kb on + strand, within RR42_RS25605 at 1089.547 kb on + strand, within RR42_RS25605 at 1089.550 kb on + strand, within RR42_RS25605 at 1089.550 kb on + strand, within RR42_RS25605 at 1089.551 kb on - strand, within RR42_RS25605 at 1089.630 kb on + strand at 1089.652 kb on + strand at 1089.827 kb on + strand at 1089.827 kb on + strand at 1089.828 kb on - strand at 1089.828 kb on - strand at 1089.828 kb on - strand at 1089.828 kb on - strand at 1089.828 kb on - strand at 1089.828 kb on - strand at 1089.828 kb on - strand at 1089.828 kb on - strand at 1089.830 kb on + strand at 1089.831 kb on - strand at 1089.875 kb on + strand at 1089.882 kb on - strand at 1089.909 kb on - strand at 1089.909 kb on - strand at 1089.947 kb on + strand, within RR42_RS25610 at 1089.947 kb on + strand, within RR42_RS25610 at 1089.998 kb on - strand, within RR42_RS25610 at 1090.025 kb on + strand, within RR42_RS25610 at 1090.031 kb on + strand, within RR42_RS25610 at 1090.031 kb on + strand, within RR42_RS25610 at 1090.031 kb on + strand, within RR42_RS25610 at 1090.061 kb on + strand, within RR42_RS25610 at 1090.197 kb on - strand, within RR42_RS25610 at 1090.197 kb on - strand, within RR42_RS25610 at 1090.197 kb on - strand, within RR42_RS25610 at 1090.197 kb on - strand, within RR42_RS25610 at 1090.294 kb on + strand, within RR42_RS25610 at 1090.294 kb on + strand, within RR42_RS25610 at 1090.294 kb on + strand, within RR42_RS25610 at 1090.450 kb on - strand, within RR42_RS25610 at 1090.673 kb on + strand at 1090.673 kb on + strand at 1090.673 kb on + strand at 1090.674 kb on - strand at 1090.674 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.01 mg/ml remove 1,087,651 + -0.4 1,087,652 - +0.9 1,087,652 - +0.3 1,087,693 + -0.3 1,087,714 - -0.3 1,088,016 + RR42_RS25600 0.39 +0.1 1,088,043 + RR42_RS25600 0.42 +1.9 1,088,043 + RR42_RS25600 0.42 +1.3 1,088,443 - +0.5 1,088,587 + -0.4 1,088,587 + +0.3 1,088,588 - +0.2 1,088,626 + +1.0 1,088,626 + +0.0 1,088,627 - -1.4 1,088,627 - +0.3 1,088,792 - RR42_RS25605 0.19 +1.4 1,088,809 + RR42_RS25605 0.20 -0.4 1,088,809 + RR42_RS25605 0.20 -0.3 1,088,809 + RR42_RS25605 0.20 +0.1 1,088,810 - RR42_RS25605 0.20 -0.7 1,088,810 - RR42_RS25605 0.20 -0.3 1,088,899 + RR42_RS25605 0.29 -0.3 1,088,935 + RR42_RS25605 0.32 +0.5 1,089,457 + RR42_RS25605 0.80 +0.7 1,089,457 + RR42_RS25605 0.80 -0.1 1,089,547 + RR42_RS25605 0.88 -0.1 1,089,550 + RR42_RS25605 0.88 +1.0 1,089,550 + RR42_RS25605 0.88 +1.6 1,089,551 - RR42_RS25605 0.88 +0.2 1,089,630 + -0.1 1,089,652 + -0.0 1,089,827 + +1.0 1,089,827 + -0.1 1,089,828 - -0.3 1,089,828 - +0.5 1,089,828 - +0.2 1,089,828 - -1.3 1,089,828 - -0.8 1,089,828 - -0.7 1,089,828 - -0.5 1,089,828 - +0.6 1,089,830 + -1.5 1,089,831 - +1.3 1,089,875 + -0.0 1,089,882 - +0.4 1,089,909 - +1.0 1,089,909 - -0.9 1,089,947 + RR42_RS25610 0.14 +0.0 1,089,947 + RR42_RS25610 0.14 +0.4 1,089,998 - RR42_RS25610 0.20 +0.5 1,090,025 + RR42_RS25610 0.23 +0.2 1,090,031 + RR42_RS25610 0.24 +0.4 1,090,031 + RR42_RS25610 0.24 +0.1 1,090,031 + RR42_RS25610 0.24 +0.6 1,090,061 + RR42_RS25610 0.28 +0.2 1,090,197 - RR42_RS25610 0.44 -0.0 1,090,197 - RR42_RS25610 0.44 -0.3 1,090,197 - RR42_RS25610 0.44 -2.7 1,090,197 - RR42_RS25610 0.44 +0.2 1,090,294 + RR42_RS25610 0.55 -1.4 1,090,294 + RR42_RS25610 0.55 -1.1 1,090,294 + RR42_RS25610 0.55 -0.7 1,090,450 - RR42_RS25610 0.74 -0.6 1,090,673 + -0.8 1,090,673 + +0.0 1,090,673 + +1.0 1,090,674 - +0.0 1,090,674 - +0.8
Or see this region's nucleotide sequence