Strain Fitness in Cupriavidus basilensis FW507-4G11 around RR42_RS25195

Experiment: R2A with D-Cycloserine 0.01 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRR42_RS25190 and RR42_RS25195 are separated by 66 nucleotidesRR42_RS25195 and RR42_RS25200 are separated by 558 nucleotides RR42_RS25190: RR42_RS25190 - acyl-CoA dehydrogenase, at 991,425 to 993,194 _RS25190 RR42_RS25195: RR42_RS25195 - AMP-dependent synthetase, at 993,261 to 994,934 _RS25195 RR42_RS25200: RR42_RS25200 - TetR family transcriptional regulator, at 995,493 to 996,071 _RS25200 Position (kb) 993 994 995Strain fitness (log2 ratio) -3 -2 -1 0 1at 992.269 kb on + strand, within RR42_RS25190at 992.375 kb on - strand, within RR42_RS25190at 992.628 kb on + strand, within RR42_RS25190at 992.629 kb on - strand, within RR42_RS25190at 992.629 kb on - strand, within RR42_RS25190at 992.752 kb on + strand, within RR42_RS25190at 992.752 kb on + strand, within RR42_RS25190at 992.753 kb on - strand, within RR42_RS25190at 992.797 kb on + strand, within RR42_RS25190at 992.797 kb on + strand, within RR42_RS25190at 992.798 kb on - strand, within RR42_RS25190at 992.841 kb on - strand, within RR42_RS25190at 992.854 kb on - strand, within RR42_RS25190at 992.953 kb on + strand, within RR42_RS25190at 992.953 kb on + strand, within RR42_RS25190at 993.078 kb on + strandat 993.079 kb on - strandat 993.184 kb on + strandat 993.184 kb on + strandat 993.185 kb on - strandat 993.185 kb on - strandat 993.185 kb on - strandat 993.187 kb on + strandat 993.188 kb on - strandat 993.188 kb on - strandat 993.188 kb on - strandat 993.195 kb on - strandat 993.195 kb on - strandat 993.263 kb on - strandat 993.353 kb on + strandat 993.353 kb on + strandat 993.372 kb on - strandat 993.372 kb on - strandat 993.869 kb on - strand, within RR42_RS25195at 993.869 kb on - strand, within RR42_RS25195at 993.973 kb on - strand, within RR42_RS25195at 994.151 kb on + strand, within RR42_RS25195at 994.151 kb on + strand, within RR42_RS25195at 994.152 kb on - strand, within RR42_RS25195at 994.156 kb on + strand, within RR42_RS25195at 994.156 kb on + strand, within RR42_RS25195at 994.156 kb on + strand, within RR42_RS25195at 994.297 kb on + strand, within RR42_RS25195at 994.297 kb on + strand, within RR42_RS25195at 994.297 kb on + strand, within RR42_RS25195at 994.464 kb on - strand, within RR42_RS25195at 994.542 kb on + strand, within RR42_RS25195at 994.542 kb on + strand, within RR42_RS25195at 994.542 kb on + strand, within RR42_RS25195at 994.560 kb on - strand, within RR42_RS25195at 994.610 kb on + strand, within RR42_RS25195at 994.611 kb on - strand, within RR42_RS25195at 994.653 kb on + strand, within RR42_RS25195at 994.704 kb on + strand, within RR42_RS25195at 994.704 kb on + strand, within RR42_RS25195at 994.705 kb on - strand, within RR42_RS25195at 994.780 kb on + strandat 994.781 kb on - strandat 994.784 kb on + strandat 994.785 kb on - strandat 994.914 kb on - strandat 994.934 kb on + strandat 994.934 kb on + strandat 994.954 kb on + strandat 994.955 kb on - strandat 994.969 kb on - strandat 995.087 kb on - strandat 995.187 kb on + strandat 995.188 kb on - strandat 995.188 kb on - strandat 995.215 kb on - strandat 995.382 kb on - strandat 995.382 kb on - strandat 995.415 kb on + strandat 995.415 kb on + strandat 995.453 kb on + strandat 995.453 kb on + strandat 995.454 kb on - strandat 995.546 kb on + strandat 995.546 kb on + strandat 995.634 kb on + strand, within RR42_RS25200at 995.634 kb on + strand, within RR42_RS25200at 995.634 kb on + strand, within RR42_RS25200at 995.635 kb on - strand, within RR42_RS25200at 995.635 kb on - strand, within RR42_RS25200at 995.636 kb on + strand, within RR42_RS25200at 995.763 kb on + strand, within RR42_RS25200at 995.764 kb on - strand, within RR42_RS25200

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Per-strain Table

Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.01 mg/ml
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992,269 + RR42_RS25190 0.48 -0.6
992,375 - RR42_RS25190 0.54 -0.1
992,628 + RR42_RS25190 0.68 +0.2
992,629 - RR42_RS25190 0.68 +1.1
992,629 - RR42_RS25190 0.68 +0.1
992,752 + RR42_RS25190 0.75 +0.1
992,752 + RR42_RS25190 0.75 +0.9
992,753 - RR42_RS25190 0.75 +0.0
992,797 + RR42_RS25190 0.78 -0.1
992,797 + RR42_RS25190 0.78 -0.8
992,798 - RR42_RS25190 0.78 -0.5
992,841 - RR42_RS25190 0.80 -0.4
992,854 - RR42_RS25190 0.81 +0.1
992,953 + RR42_RS25190 0.86 +0.6
992,953 + RR42_RS25190 0.86 -1.5
993,078 + -0.2
993,079 - -0.7
993,184 + +0.1
993,184 + +0.0
993,185 - +0.2
993,185 - +0.2
993,185 - +0.5
993,187 + +0.2
993,188 - -0.5
993,188 - +0.4
993,188 - -0.9
993,195 - +0.2
993,195 - -1.3
993,263 - -0.9
993,353 + -0.3
993,353 + -0.7
993,372 - +0.1
993,372 - -0.7
993,869 - RR42_RS25195 0.36 +0.4
993,869 - RR42_RS25195 0.36 +0.4
993,973 - RR42_RS25195 0.43 +0.3
994,151 + RR42_RS25195 0.53 +0.8
994,151 + RR42_RS25195 0.53 +0.3
994,152 - RR42_RS25195 0.53 +0.2
994,156 + RR42_RS25195 0.53 +0.1
994,156 + RR42_RS25195 0.53 +0.3
994,156 + RR42_RS25195 0.53 -0.6
994,297 + RR42_RS25195 0.62 +0.1
994,297 + RR42_RS25195 0.62 +0.2
994,297 + RR42_RS25195 0.62 -0.0
994,464 - RR42_RS25195 0.72 +0.4
994,542 + RR42_RS25195 0.77 +0.3
994,542 + RR42_RS25195 0.77 +0.8
994,542 + RR42_RS25195 0.77 +0.3
994,560 - RR42_RS25195 0.78 -0.6
994,610 + RR42_RS25195 0.81 +0.8
994,611 - RR42_RS25195 0.81 +0.4
994,653 + RR42_RS25195 0.83 +0.2
994,704 + RR42_RS25195 0.86 +0.5
994,704 + RR42_RS25195 0.86 +0.4
994,705 - RR42_RS25195 0.86 -0.2
994,780 + -0.3
994,781 - +0.0
994,784 + +0.4
994,785 - -0.4
994,914 - -0.9
994,934 + -0.7
994,934 + +0.3
994,954 + +0.2
994,955 - +0.1
994,969 - +0.0
995,087 - +0.3
995,187 + +0.1
995,188 - +0.3
995,188 - +0.2
995,215 - -0.1
995,382 - -0.1
995,382 - +0.5
995,415 + +0.1
995,415 + -0.1
995,453 + -0.4
995,453 + +0.8
995,454 - +0.9
995,546 + -2.0
995,546 + -0.2
995,634 + RR42_RS25200 0.24 -0.6
995,634 + RR42_RS25200 0.24 -0.1
995,634 + RR42_RS25200 0.24 -2.8
995,635 - RR42_RS25200 0.25 +0.1
995,635 - RR42_RS25200 0.25 +0.4
995,636 + RR42_RS25200 0.25 -0.2
995,763 + RR42_RS25200 0.47 +1.1
995,764 - RR42_RS25200 0.47 -2.1

Or see this region's nucleotide sequence