Experiment: R2A with D-Cycloserine 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS21950 and RR42_RS21955 are separated by 19 nucleotides RR42_RS21955 and RR42_RS21960 are separated by 195 nucleotides
RR42_RS21950: RR42_RS21950 - hypothetical protein, at 250,144 to 251,877
_RS21950
RR42_RS21955: RR42_RS21955 - hypothetical protein, at 251,897 to 252,097
_RS21955
RR42_RS21960: RR42_RS21960 - cysteine synthase, at 252,293 to 253,210
_RS21960
Position (kb) 251
252
253 Strain fitness (log2 ratio) -2
-1
0
1 at 251.331 kb on + strand, within RR42_RS21950 at 251.331 kb on + strand, within RR42_RS21950 at 251.331 kb on + strand, within RR42_RS21950 at 251.331 kb on + strand, within RR42_RS21950 at 251.332 kb on - strand, within RR42_RS21950 at 251.332 kb on - strand, within RR42_RS21950 at 251.332 kb on - strand, within RR42_RS21950 at 251.332 kb on - strand, within RR42_RS21950 at 251.382 kb on + strand, within RR42_RS21950 at 251.383 kb on - strand, within RR42_RS21950 at 251.383 kb on - strand, within RR42_RS21950 at 251.383 kb on - strand, within RR42_RS21950 at 251.383 kb on - strand, within RR42_RS21950 at 251.504 kb on - strand, within RR42_RS21950 at 251.702 kb on + strand, within RR42_RS21950 at 251.789 kb on - strand at 251.854 kb on + strand at 251.854 kb on + strand at 251.854 kb on + strand at 251.855 kb on - strand at 251.855 kb on - strand at 251.855 kb on - strand at 251.898 kb on + strand at 251.898 kb on + strand at 251.899 kb on - strand at 252.039 kb on - strand, within RR42_RS21955 at 252.039 kb on - strand, within RR42_RS21955 at 252.081 kb on + strand at 252.081 kb on + strand at 252.082 kb on - strand at 252.082 kb on - strand at 252.082 kb on - strand at 252.239 kb on - strand at 252.239 kb on - strand at 252.239 kb on - strand at 252.239 kb on - strand at 252.324 kb on + strand at 252.325 kb on - strand at 252.549 kb on - strand, within RR42_RS21960 at 252.618 kb on + strand, within RR42_RS21960 at 252.619 kb on - strand, within RR42_RS21960 at 252.670 kb on + strand, within RR42_RS21960 at 252.671 kb on - strand, within RR42_RS21960 at 252.671 kb on - strand, within RR42_RS21960 at 252.766 kb on + strand, within RR42_RS21960 at 252.891 kb on + strand, within RR42_RS21960 at 252.955 kb on - strand, within RR42_RS21960 at 252.955 kb on - strand, within RR42_RS21960 at 252.995 kb on - strand, within RR42_RS21960
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.01 mg/ml remove 251,331 + RR42_RS21950 0.68 +0.1 251,331 + RR42_RS21950 0.68 +0.1 251,331 + RR42_RS21950 0.68 -0.1 251,331 + RR42_RS21950 0.68 -0.3 251,332 - RR42_RS21950 0.69 -0.2 251,332 - RR42_RS21950 0.69 -0.1 251,332 - RR42_RS21950 0.69 +0.9 251,332 - RR42_RS21950 0.69 -0.8 251,382 + RR42_RS21950 0.71 -0.2 251,383 - RR42_RS21950 0.71 -0.1 251,383 - RR42_RS21950 0.71 -0.3 251,383 - RR42_RS21950 0.71 -0.4 251,383 - RR42_RS21950 0.71 -0.6 251,504 - RR42_RS21950 0.78 -0.2 251,702 + RR42_RS21950 0.90 -0.1 251,789 - -0.1 251,854 + -0.8 251,854 + -1.5 251,854 + +0.4 251,855 - -0.3 251,855 - +0.3 251,855 - +0.8 251,898 + -0.8 251,898 + -0.5 251,899 - -0.7 252,039 - RR42_RS21955 0.71 -1.4 252,039 - RR42_RS21955 0.71 -1.4 252,081 + +0.6 252,081 + -0.3 252,082 - +0.4 252,082 - -0.2 252,082 - +0.6 252,239 - -0.2 252,239 - +0.3 252,239 - +0.7 252,239 - +0.2 252,324 + +0.6 252,325 - -0.2 252,549 - RR42_RS21960 0.28 -0.1 252,618 + RR42_RS21960 0.35 +1.1 252,619 - RR42_RS21960 0.36 +0.2 252,670 + RR42_RS21960 0.41 -0.1 252,671 - RR42_RS21960 0.41 -0.9 252,671 - RR42_RS21960 0.41 -2.0 252,766 + RR42_RS21960 0.52 -0.6 252,891 + RR42_RS21960 0.65 +0.8 252,955 - RR42_RS21960 0.72 +0.4 252,955 - RR42_RS21960 0.72 +0.6 252,995 - RR42_RS21960 0.76 +0.6
Or see this region's nucleotide sequence