Experiment: R2A with D-Cycloserine 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS21430 and RR42_RS21435 are separated by 345 nucleotides RR42_RS21435 and RR42_RS21440 are separated by 222 nucleotides
RR42_RS21430: RR42_RS21430 - alpha-ketoglutarate permease, at 136,976 to 138,286
_RS21430
RR42_RS21435: RR42_RS21435 - hypothetical protein, at 138,632 to 139,015
_RS21435
RR42_RS21440: RR42_RS21440 - DNA-3-methyladenine glycosylase, at 139,238 to 142,051
_RS21440
Position (kb)
138
139
140 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 137.839 kb on + strand, within RR42_RS21430 at 137.839 kb on + strand, within RR42_RS21430 at 137.839 kb on + strand, within RR42_RS21430 at 137.839 kb on + strand, within RR42_RS21430 at 137.839 kb on + strand, within RR42_RS21430 at 137.839 kb on + strand, within RR42_RS21430 at 137.840 kb on - strand, within RR42_RS21430 at 137.840 kb on - strand, within RR42_RS21430 at 137.840 kb on - strand, within RR42_RS21430 at 137.840 kb on - strand, within RR42_RS21430 at 137.841 kb on + strand, within RR42_RS21430 at 137.841 kb on + strand, within RR42_RS21430 at 137.841 kb on + strand, within RR42_RS21430 at 137.841 kb on + strand, within RR42_RS21430 at 137.841 kb on + strand, within RR42_RS21430 at 137.841 kb on + strand, within RR42_RS21430 at 137.842 kb on - strand, within RR42_RS21430 at 137.842 kb on - strand, within RR42_RS21430 at 137.842 kb on - strand, within RR42_RS21430 at 137.842 kb on - strand, within RR42_RS21430 at 137.842 kb on - strand, within RR42_RS21430 at 137.860 kb on - strand, within RR42_RS21430 at 137.937 kb on + strand, within RR42_RS21430 at 137.937 kb on + strand, within RR42_RS21430 at 137.938 kb on - strand, within RR42_RS21430 at 137.938 kb on - strand, within RR42_RS21430 at 138.144 kb on + strand, within RR42_RS21430 at 138.169 kb on - strand at 138.169 kb on - strand at 138.220 kb on - strand at 138.321 kb on + strand at 138.321 kb on + strand at 138.322 kb on - strand at 138.362 kb on + strand at 138.363 kb on - strand at 138.409 kb on + strand at 138.409 kb on + strand at 138.410 kb on - strand at 138.410 kb on - strand at 138.410 kb on - strand at 138.410 kb on - strand at 138.594 kb on + strand at 138.594 kb on + strand at 138.595 kb on - strand at 138.595 kb on - strand at 138.662 kb on + strand at 138.693 kb on + strand, within RR42_RS21435 at 138.771 kb on + strand, within RR42_RS21435 at 138.933 kb on + strand, within RR42_RS21435 at 138.933 kb on + strand, within RR42_RS21435 at 138.997 kb on + strand at 138.998 kb on - strand at 138.998 kb on - strand at 139.026 kb on + strand at 139.026 kb on + strand at 139.026 kb on + strand at 139.026 kb on + strand at 139.026 kb on + strand at 139.027 kb on - strand at 139.027 kb on - strand at 139.027 kb on - strand at 139.027 kb on - strand at 139.027 kb on - strand at 139.029 kb on - strand at 139.029 kb on - strand at 139.029 kb on - strand at 139.038 kb on + strand at 139.051 kb on + strand at 139.101 kb on + strand at 139.101 kb on + strand at 139.101 kb on + strand at 139.102 kb on - strand at 139.102 kb on - strand at 139.154 kb on + strand at 139.154 kb on + strand at 139.154 kb on + strand at 139.154 kb on + strand at 139.155 kb on - strand at 139.155 kb on - strand at 139.155 kb on - strand at 139.232 kb on + strand at 139.232 kb on + strand at 139.281 kb on + strand at 139.281 kb on + strand at 139.281 kb on + strand at 139.282 kb on - strand at 139.289 kb on + strand at 139.289 kb on + strand at 139.289 kb on + strand at 139.289 kb on + strand at 139.290 kb on - strand at 139.290 kb on - strand at 139.290 kb on - strand at 139.290 kb on - strand at 139.290 kb on - strand at 139.357 kb on + strand at 139.357 kb on + strand at 139.358 kb on - strand at 139.368 kb on + strand at 139.368 kb on + strand at 139.368 kb on + strand at 139.369 kb on - strand at 139.403 kb on + strand at 139.403 kb on + strand at 139.403 kb on + strand at 139.404 kb on - strand at 139.404 kb on - strand at 139.404 kb on - strand at 139.647 kb on + strand, within RR42_RS21440 at 139.647 kb on + strand, within RR42_RS21440 at 139.648 kb on - strand, within RR42_RS21440 at 139.648 kb on - strand, within RR42_RS21440 at 139.648 kb on - strand, within RR42_RS21440 at 139.648 kb on - strand, within RR42_RS21440 at 139.648 kb on - strand, within RR42_RS21440 at 139.648 kb on - strand, within RR42_RS21440 at 139.785 kb on + strand, within RR42_RS21440 at 139.785 kb on + strand, within RR42_RS21440 at 139.785 kb on + strand, within RR42_RS21440 at 139.786 kb on - strand, within RR42_RS21440 at 139.786 kb on - strand, within RR42_RS21440 at 139.786 kb on - strand, within RR42_RS21440
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.01 mg/ml remove 137,839 + RR42_RS21430 0.66 -0.4 137,839 + RR42_RS21430 0.66 -0.2 137,839 + RR42_RS21430 0.66 -0.2 137,839 + RR42_RS21430 0.66 +0.6 137,839 + RR42_RS21430 0.66 +0.1 137,839 + RR42_RS21430 0.66 +0.6 137,840 - RR42_RS21430 0.66 -0.3 137,840 - RR42_RS21430 0.66 +0.3 137,840 - RR42_RS21430 0.66 +1.1 137,840 - RR42_RS21430 0.66 -0.2 137,841 + RR42_RS21430 0.66 +0.2 137,841 + RR42_RS21430 0.66 -0.9 137,841 + RR42_RS21430 0.66 -0.7 137,841 + RR42_RS21430 0.66 +1.4 137,841 + RR42_RS21430 0.66 +0.5 137,841 + RR42_RS21430 0.66 -0.3 137,842 - RR42_RS21430 0.66 -0.0 137,842 - RR42_RS21430 0.66 -0.1 137,842 - RR42_RS21430 0.66 -0.6 137,842 - RR42_RS21430 0.66 +0.1 137,842 - RR42_RS21430 0.66 +0.1 137,860 - RR42_RS21430 0.67 +0.4 137,937 + RR42_RS21430 0.73 -0.3 137,937 + RR42_RS21430 0.73 +1.0 137,938 - RR42_RS21430 0.73 +0.2 137,938 - RR42_RS21430 0.73 -0.6 138,144 + RR42_RS21430 0.89 +0.5 138,169 - -1.6 138,169 - -0.3 138,220 - -0.2 138,321 + +1.1 138,321 + -0.5 138,322 - +0.6 138,362 + +0.4 138,363 - +0.3 138,409 + +0.4 138,409 + -1.1 138,410 - -1.7 138,410 - +0.5 138,410 - -0.6 138,410 - -1.0 138,594 + -0.4 138,594 + +0.1 138,595 - +0.4 138,595 - -0.2 138,662 + +0.6 138,693 + RR42_RS21435 0.16 -0.2 138,771 + RR42_RS21435 0.36 -1.4 138,933 + RR42_RS21435 0.78 -0.0 138,933 + RR42_RS21435 0.78 -0.7 138,997 + -0.1 138,998 - -0.8 138,998 - -0.4 139,026 + +0.4 139,026 + +0.1 139,026 + -0.4 139,026 + +1.4 139,026 + -0.4 139,027 - -0.1 139,027 - +0.6 139,027 - +1.4 139,027 - -0.6 139,027 - -0.6 139,029 - -0.9 139,029 - -0.1 139,029 - +1.8 139,038 + +0.0 139,051 + -0.6 139,101 + -0.2 139,101 + -0.1 139,101 + +0.4 139,102 - +0.3 139,102 - +0.5 139,154 + -0.9 139,154 + +0.6 139,154 + -0.2 139,154 + +0.2 139,155 - -2.2 139,155 - +0.8 139,155 - -0.5 139,232 + -1.4 139,232 + -0.6 139,281 + -1.2 139,281 + +0.1 139,281 + -0.8 139,282 - +0.7 139,289 + -0.4 139,289 + +0.0 139,289 + -0.2 139,289 + +0.1 139,290 - -0.6 139,290 - +0.8 139,290 - +1.5 139,290 - +0.4 139,290 - -0.3 139,357 + +1.1 139,357 + -0.3 139,358 - +0.5 139,368 + +0.1 139,368 + +0.7 139,368 + +0.4 139,369 - +0.4 139,403 + +0.6 139,403 + +1.4 139,403 + -0.0 139,404 - +0.3 139,404 - -0.3 139,404 - -0.6 139,647 + RR42_RS21440 0.15 +0.4 139,647 + RR42_RS21440 0.15 -1.6 139,648 - RR42_RS21440 0.15 -0.6 139,648 - RR42_RS21440 0.15 +1.0 139,648 - RR42_RS21440 0.15 -0.9 139,648 - RR42_RS21440 0.15 +0.2 139,648 - RR42_RS21440 0.15 +0.2 139,648 - RR42_RS21440 0.15 +0.7 139,785 + RR42_RS21440 0.19 +0.6 139,785 + RR42_RS21440 0.19 -0.4 139,785 + RR42_RS21440 0.19 -2.0 139,786 - RR42_RS21440 0.19 +0.7 139,786 - RR42_RS21440 0.19 +1.0 139,786 - RR42_RS21440 0.19 -0.2
Or see this region's nucleotide sequence