Experiment: R2A with D-Cycloserine 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS21120 and RR42_RS21125 are separated by 249 nucleotides RR42_RS21125 and RR42_RS21130 are separated by 248 nucleotides
RR42_RS21120: RR42_RS21120 - acetate kinase, at 63,333 to 64,745
_RS21120
RR42_RS21125: RR42_RS21125 - membrane protein, at 64,995 to 65,507
_RS21125
RR42_RS21130: RR42_RS21130 - ABC transporter permease, at 65,756 to 66,892
_RS21130
Position (kb) 64
65
66 Strain fitness (log2 ratio) -2
-1
0
1
2 at 64.025 kb on - strand, within RR42_RS21120 at 64.025 kb on - strand, within RR42_RS21120 at 64.025 kb on - strand, within RR42_RS21120 at 64.025 kb on - strand, within RR42_RS21120 at 64.042 kb on - strand, within RR42_RS21120 at 64.155 kb on + strand, within RR42_RS21120 at 64.167 kb on + strand, within RR42_RS21120 at 64.168 kb on - strand, within RR42_RS21120 at 64.168 kb on - strand, within RR42_RS21120 at 64.209 kb on + strand, within RR42_RS21120 at 64.210 kb on - strand, within RR42_RS21120 at 64.210 kb on - strand, within RR42_RS21120 at 64.347 kb on - strand, within RR42_RS21120 at 64.395 kb on + strand, within RR42_RS21120 at 64.395 kb on + strand, within RR42_RS21120 at 64.395 kb on + strand, within RR42_RS21120 at 64.635 kb on + strand at 64.660 kb on - strand at 64.827 kb on - strand at 64.827 kb on - strand at 64.842 kb on + strand at 64.843 kb on - strand at 65.006 kb on + strand at 65.006 kb on + strand at 65.007 kb on - strand at 65.007 kb on - strand at 65.007 kb on - strand at 65.117 kb on + strand, within RR42_RS21125 at 65.118 kb on - strand, within RR42_RS21125 at 65.614 kb on - strand at 65.681 kb on + strand at 65.682 kb on - strand at 65.682 kb on - strand at 65.931 kb on - strand, within RR42_RS21130 at 65.931 kb on - strand, within RR42_RS21130 at 65.962 kb on - strand, within RR42_RS21130 at 65.990 kb on + strand, within RR42_RS21130 at 65.990 kb on + strand, within RR42_RS21130 at 65.991 kb on - strand, within RR42_RS21130 at 65.991 kb on - strand, within RR42_RS21130 at 66.076 kb on - strand, within RR42_RS21130 at 66.198 kb on + strand, within RR42_RS21130 at 66.198 kb on + strand, within RR42_RS21130 at 66.198 kb on + strand, within RR42_RS21130 at 66.199 kb on - strand, within RR42_RS21130 at 66.209 kb on + strand, within RR42_RS21130 at 66.279 kb on - strand, within RR42_RS21130
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.01 mg/ml remove 64,025 - RR42_RS21120 0.49 -0.8 64,025 - RR42_RS21120 0.49 +0.2 64,025 - RR42_RS21120 0.49 +0.4 64,025 - RR42_RS21120 0.49 +0.5 64,042 - RR42_RS21120 0.50 +0.6 64,155 + RR42_RS21120 0.58 -1.2 64,167 + RR42_RS21120 0.59 -0.7 64,168 - RR42_RS21120 0.59 +0.3 64,168 - RR42_RS21120 0.59 -0.8 64,209 + RR42_RS21120 0.62 +1.4 64,210 - RR42_RS21120 0.62 -0.6 64,210 - RR42_RS21120 0.62 -0.1 64,347 - RR42_RS21120 0.72 +0.1 64,395 + RR42_RS21120 0.75 -0.2 64,395 + RR42_RS21120 0.75 +0.4 64,395 + RR42_RS21120 0.75 -0.1 64,635 + +0.8 64,660 - -0.4 64,827 - -0.2 64,827 - -0.8 64,842 + +0.6 64,843 - -0.3 65,006 + -0.6 65,006 + +1.0 65,007 - -0.4 65,007 - -0.7 65,007 - -0.3 65,117 + RR42_RS21125 0.24 -1.4 65,118 - RR42_RS21125 0.24 -0.1 65,614 - -0.4 65,681 + +0.4 65,682 - -0.2 65,682 - +0.9 65,931 - RR42_RS21130 0.15 -1.1 65,931 - RR42_RS21130 0.15 -0.5 65,962 - RR42_RS21130 0.18 +0.4 65,990 + RR42_RS21130 0.21 -0.0 65,990 + RR42_RS21130 0.21 -0.4 65,991 - RR42_RS21130 0.21 +0.5 65,991 - RR42_RS21130 0.21 -0.4 66,076 - RR42_RS21130 0.28 +2.4 66,198 + RR42_RS21130 0.39 -2.6 66,198 + RR42_RS21130 0.39 -0.3 66,198 + RR42_RS21130 0.39 +0.8 66,199 - RR42_RS21130 0.39 -0.0 66,209 + RR42_RS21130 0.40 +0.7 66,279 - RR42_RS21130 0.46 +0.3
Or see this region's nucleotide sequence