Experiment: R2A with D-Cycloserine 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS20250 and RR42_RS20255 are separated by 407 nucleotides RR42_RS20255 and RR42_RS20260 are separated by 81 nucleotides RR42_RS20260 and RR42_RS20265 are separated by 277 nucleotides
RR42_RS20250: RR42_RS20250 - branched-chain amino acid ABC transporter substrate-binding protein, at 4,380,568 to 4,381,719
_RS20250
RR42_RS20255: RR42_RS20255 - membrane protein, at 4,382,127 to 4,382,885
_RS20255
RR42_RS20260: RR42_RS20260 - hypothetical protein, at 4,382,967 to 4,383,518
_RS20260
RR42_RS20265: RR42_RS20265 - ABC transporter permease, at 4,383,796 to 4,384,824
_RS20265
Position (kb)
4382
4383 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 4381.217 kb on + strand, within RR42_RS20250 at 4381.217 kb on + strand, within RR42_RS20250 at 4381.217 kb on + strand, within RR42_RS20250 at 4381.218 kb on - strand, within RR42_RS20250 at 4381.218 kb on - strand, within RR42_RS20250 at 4381.236 kb on + strand, within RR42_RS20250 at 4381.450 kb on + strand, within RR42_RS20250 at 4381.450 kb on + strand, within RR42_RS20250 at 4381.450 kb on + strand, within RR42_RS20250 at 4381.451 kb on - strand, within RR42_RS20250 at 4381.451 kb on - strand, within RR42_RS20250 at 4381.451 kb on - strand, within RR42_RS20250 at 4381.451 kb on - strand, within RR42_RS20250 at 4381.535 kb on + strand, within RR42_RS20250 at 4381.536 kb on - strand, within RR42_RS20250 at 4381.633 kb on + strand at 4381.634 kb on - strand at 4381.824 kb on + strand at 4381.824 kb on + strand at 4381.824 kb on + strand at 4381.825 kb on - strand at 4381.825 kb on - strand at 4381.916 kb on + strand at 4382.051 kb on - strand at 4382.051 kb on - strand at 4382.062 kb on + strand at 4382.256 kb on - strand, within RR42_RS20255 at 4382.277 kb on + strand, within RR42_RS20255 at 4382.277 kb on + strand, within RR42_RS20255 at 4382.278 kb on - strand, within RR42_RS20255 at 4382.446 kb on + strand, within RR42_RS20255 at 4382.446 kb on + strand, within RR42_RS20255 at 4382.446 kb on + strand, within RR42_RS20255 at 4382.546 kb on + strand, within RR42_RS20255 at 4382.546 kb on + strand, within RR42_RS20255 at 4382.546 kb on + strand, within RR42_RS20255 at 4382.547 kb on - strand, within RR42_RS20255 at 4382.547 kb on - strand, within RR42_RS20255 at 4382.547 kb on - strand, within RR42_RS20255 at 4382.549 kb on - strand, within RR42_RS20255 at 4382.549 kb on - strand, within RR42_RS20255 at 4382.571 kb on - strand, within RR42_RS20255 at 4382.571 kb on - strand, within RR42_RS20255 at 4382.621 kb on - strand, within RR42_RS20255 at 4382.680 kb on + strand, within RR42_RS20255 at 4382.680 kb on + strand, within RR42_RS20255 at 4382.681 kb on - strand, within RR42_RS20255 at 4382.681 kb on - strand, within RR42_RS20255 at 4382.681 kb on - strand, within RR42_RS20255 at 4382.681 kb on - strand, within RR42_RS20255 at 4382.701 kb on + strand, within RR42_RS20255 at 4382.724 kb on - strand, within RR42_RS20255 at 4382.724 kb on - strand, within RR42_RS20255 at 4382.724 kb on - strand, within RR42_RS20255 at 4382.724 kb on - strand, within RR42_RS20255 at 4382.724 kb on - strand, within RR42_RS20255 at 4382.976 kb on + strand at 4382.976 kb on + strand at 4382.977 kb on - strand at 4383.226 kb on - strand, within RR42_RS20260 at 4383.420 kb on + strand, within RR42_RS20260 at 4383.420 kb on + strand, within RR42_RS20260 at 4383.421 kb on - strand, within RR42_RS20260 at 4383.582 kb on + strand at 4383.583 kb on - strand at 4383.583 kb on - strand at 4383.634 kb on + strand at 4383.635 kb on - strand at 4383.635 kb on - strand at 4383.635 kb on - strand at 4383.635 kb on - strand at 4383.639 kb on + strand at 4383.639 kb on + strand at 4383.640 kb on - strand at 4383.641 kb on + strand at 4383.642 kb on - strand at 4383.642 kb on - strand at 4383.657 kb on + strand at 4383.657 kb on + strand at 4383.699 kb on + strand at 4383.700 kb on - strand at 4383.715 kb on + strand at 4383.715 kb on + strand at 4383.716 kb on - strand at 4383.718 kb on - strand at 4383.726 kb on + strand at 4383.726 kb on + strand at 4383.727 kb on - strand at 4383.727 kb on - strand at 4383.753 kb on + strand at 4383.859 kb on + strand at 4383.860 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.01 mg/ml remove 4,381,217 + RR42_RS20250 0.56 -1.3 4,381,217 + RR42_RS20250 0.56 -0.1 4,381,217 + RR42_RS20250 0.56 -0.0 4,381,218 - RR42_RS20250 0.56 +0.2 4,381,218 - RR42_RS20250 0.56 +0.6 4,381,236 + RR42_RS20250 0.58 -0.4 4,381,450 + RR42_RS20250 0.77 -0.4 4,381,450 + RR42_RS20250 0.77 -0.3 4,381,450 + RR42_RS20250 0.77 +0.3 4,381,451 - RR42_RS20250 0.77 -0.4 4,381,451 - RR42_RS20250 0.77 +0.5 4,381,451 - RR42_RS20250 0.77 -0.7 4,381,451 - RR42_RS20250 0.77 -2.2 4,381,535 + RR42_RS20250 0.84 +0.2 4,381,536 - RR42_RS20250 0.84 +0.0 4,381,633 + +0.2 4,381,634 - +2.9 4,381,824 + -0.4 4,381,824 + -0.9 4,381,824 + +1.6 4,381,825 - -0.6 4,381,825 - -0.1 4,381,916 + +2.2 4,382,051 - +0.0 4,382,051 - +0.4 4,382,062 + -0.3 4,382,256 - RR42_RS20255 0.17 -1.9 4,382,277 + RR42_RS20255 0.20 -0.4 4,382,277 + RR42_RS20255 0.20 -0.5 4,382,278 - RR42_RS20255 0.20 -0.4 4,382,446 + RR42_RS20255 0.42 +0.6 4,382,446 + RR42_RS20255 0.42 -0.6 4,382,446 + RR42_RS20255 0.42 +0.1 4,382,546 + RR42_RS20255 0.55 -0.1 4,382,546 + RR42_RS20255 0.55 +1.0 4,382,546 + RR42_RS20255 0.55 +0.3 4,382,547 - RR42_RS20255 0.55 +0.7 4,382,547 - RR42_RS20255 0.55 -0.5 4,382,547 - RR42_RS20255 0.55 -0.7 4,382,549 - RR42_RS20255 0.56 -1.0 4,382,549 - RR42_RS20255 0.56 -1.3 4,382,571 - RR42_RS20255 0.58 +1.7 4,382,571 - RR42_RS20255 0.58 -0.3 4,382,621 - RR42_RS20255 0.65 -1.2 4,382,680 + RR42_RS20255 0.73 +0.1 4,382,680 + RR42_RS20255 0.73 +1.3 4,382,681 - RR42_RS20255 0.73 -0.3 4,382,681 - RR42_RS20255 0.73 -1.2 4,382,681 - RR42_RS20255 0.73 +0.1 4,382,681 - RR42_RS20255 0.73 -0.6 4,382,701 + RR42_RS20255 0.76 -0.3 4,382,724 - RR42_RS20255 0.79 -1.8 4,382,724 - RR42_RS20255 0.79 +0.3 4,382,724 - RR42_RS20255 0.79 +0.1 4,382,724 - RR42_RS20255 0.79 +0.4 4,382,724 - RR42_RS20255 0.79 -0.5 4,382,976 + +0.7 4,382,976 + +0.1 4,382,977 - -0.4 4,383,226 - RR42_RS20260 0.47 +0.2 4,383,420 + RR42_RS20260 0.82 -1.0 4,383,420 + RR42_RS20260 0.82 -0.5 4,383,421 - RR42_RS20260 0.82 -0.6 4,383,582 + -1.2 4,383,583 - -0.2 4,383,583 - -1.2 4,383,634 + +1.3 4,383,635 - +0.5 4,383,635 - -1.3 4,383,635 - -0.0 4,383,635 - +0.1 4,383,639 + -0.6 4,383,639 + +1.2 4,383,640 - -0.1 4,383,641 + -0.2 4,383,642 - +0.5 4,383,642 - -0.1 4,383,657 + +0.3 4,383,657 + +0.3 4,383,699 + -0.2 4,383,700 - +0.0 4,383,715 + -0.6 4,383,715 + +0.6 4,383,716 - +0.2 4,383,718 - +0.1 4,383,726 + +0.7 4,383,726 + +0.1 4,383,727 - -0.1 4,383,727 - -0.5 4,383,753 + +0.7 4,383,859 + -0.0 4,383,860 - +0.8
Or see this region's nucleotide sequence