Experiment: R2A with D-Cycloserine 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS19550 and RR42_RS19555 are separated by 138 nucleotides RR42_RS19555 and RR42_RS19560 are separated by 59 nucleotides RR42_RS19560 and RR42_RS19565 overlap by 4 nucleotides
RR42_RS19550: RR42_RS19550 - hypothetical protein, at 4,226,496 to 4,227,194
_RS19550
RR42_RS19555: RR42_RS19555 - hypothetical protein, at 4,227,333 to 4,227,704
_RS19555
RR42_RS19560: RR42_RS19560 - hypothetical protein, at 4,227,764 to 4,228,375
_RS19560
RR42_RS19565: RR42_RS19565 - sugar nucleotide epimerase YfcH family protein, at 4,228,372 to 4,229,883
_RS19565
Position (kb) 4227
4228 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 4226.433 kb on + strand at 4226.433 kb on + strand at 4226.433 kb on + strand at 4226.433 kb on + strand at 4226.434 kb on - strand at 4226.434 kb on - strand at 4226.434 kb on - strand at 4226.434 kb on - strand at 4226.576 kb on - strand, within RR42_RS19550 at 4226.576 kb on - strand, within RR42_RS19550 at 4226.631 kb on + strand, within RR42_RS19550 at 4226.631 kb on + strand, within RR42_RS19550 at 4226.737 kb on + strand, within RR42_RS19550 at 4226.738 kb on - strand, within RR42_RS19550 at 4226.738 kb on - strand, within RR42_RS19550 at 4226.738 kb on - strand, within RR42_RS19550 at 4226.895 kb on - strand, within RR42_RS19550 at 4226.941 kb on - strand, within RR42_RS19550 at 4226.962 kb on + strand, within RR42_RS19550 at 4226.962 kb on + strand, within RR42_RS19550 at 4226.963 kb on - strand, within RR42_RS19550 at 4227.020 kb on + strand, within RR42_RS19550 at 4227.021 kb on - strand, within RR42_RS19550 at 4227.021 kb on - strand, within RR42_RS19550 at 4227.115 kb on + strand, within RR42_RS19550 at 4227.115 kb on + strand, within RR42_RS19550 at 4227.209 kb on + strand at 4227.211 kb on + strand at 4227.237 kb on + strand at 4227.237 kb on + strand at 4227.237 kb on + strand at 4227.238 kb on - strand at 4227.257 kb on - strand at 4227.308 kb on + strand at 4227.332 kb on - strand at 4227.332 kb on - strand at 4227.661 kb on + strand, within RR42_RS19555 at 4227.662 kb on - strand, within RR42_RS19555 at 4227.671 kb on + strand at 4227.672 kb on - strand at 4227.673 kb on + strand at 4227.673 kb on + strand at 4227.673 kb on + strand at 4227.673 kb on + strand at 4227.673 kb on + strand at 4227.674 kb on - strand at 4227.674 kb on - strand at 4227.674 kb on - strand at 4227.674 kb on - strand at 4227.674 kb on - strand at 4227.752 kb on + strand at 4227.753 kb on - strand at 4227.753 kb on - strand at 4227.753 kb on - strand at 4227.783 kb on + strand at 4227.784 kb on - strand at 4227.784 kb on - strand at 4227.843 kb on + strand, within RR42_RS19560 at 4227.843 kb on + strand, within RR42_RS19560 at 4227.844 kb on - strand, within RR42_RS19560 at 4227.855 kb on + strand, within RR42_RS19560 at 4227.856 kb on - strand, within RR42_RS19560 at 4227.895 kb on - strand, within RR42_RS19560 at 4228.326 kb on + strand at 4228.326 kb on + strand at 4228.364 kb on + strand at 4228.443 kb on - strand at 4228.443 kb on - strand at 4228.624 kb on - strand, within RR42_RS19565 at 4228.624 kb on - strand, within RR42_RS19565 at 4228.625 kb on + strand, within RR42_RS19565 at 4228.625 kb on + strand, within RR42_RS19565 at 4228.626 kb on - strand, within RR42_RS19565 at 4228.626 kb on - strand, within RR42_RS19565 at 4228.626 kb on - strand, within RR42_RS19565 at 4228.626 kb on - strand, within RR42_RS19565
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.01 mg/ml remove 4,226,433 + -0.0 4,226,433 + -0.1 4,226,433 + -0.3 4,226,433 + +0.1 4,226,434 - +1.2 4,226,434 - -0.3 4,226,434 - -0.6 4,226,434 - +0.2 4,226,576 - RR42_RS19550 0.11 +0.2 4,226,576 - RR42_RS19550 0.11 +1.3 4,226,631 + RR42_RS19550 0.19 -0.2 4,226,631 + RR42_RS19550 0.19 +1.6 4,226,737 + RR42_RS19550 0.34 +0.5 4,226,738 - RR42_RS19550 0.35 +0.2 4,226,738 - RR42_RS19550 0.35 +0.9 4,226,738 - RR42_RS19550 0.35 -0.3 4,226,895 - RR42_RS19550 0.57 +0.2 4,226,941 - RR42_RS19550 0.64 +0.8 4,226,962 + RR42_RS19550 0.67 -0.4 4,226,962 + RR42_RS19550 0.67 -0.7 4,226,963 - RR42_RS19550 0.67 +0.9 4,227,020 + RR42_RS19550 0.75 +0.6 4,227,021 - RR42_RS19550 0.75 -0.0 4,227,021 - RR42_RS19550 0.75 -0.6 4,227,115 + RR42_RS19550 0.89 +0.4 4,227,115 + RR42_RS19550 0.89 -0.3 4,227,209 + +0.6 4,227,211 + -0.4 4,227,237 + +0.2 4,227,237 + +0.1 4,227,237 + +0.3 4,227,238 - -1.9 4,227,257 - -0.8 4,227,308 + +0.3 4,227,332 - -0.2 4,227,332 - +0.3 4,227,661 + RR42_RS19555 0.88 -1.0 4,227,662 - RR42_RS19555 0.88 +0.8 4,227,671 + +0.2 4,227,672 - -0.4 4,227,673 + +0.1 4,227,673 + +0.2 4,227,673 + -1.0 4,227,673 + -0.0 4,227,673 + +0.3 4,227,674 - -0.8 4,227,674 - -0.1 4,227,674 - -1.9 4,227,674 - -0.4 4,227,674 - -0.6 4,227,752 + -0.3 4,227,753 - -0.7 4,227,753 - -1.6 4,227,753 - -1.2 4,227,783 + +0.9 4,227,784 - +0.2 4,227,784 - +0.1 4,227,843 + RR42_RS19560 0.13 -0.5 4,227,843 + RR42_RS19560 0.13 +0.2 4,227,844 - RR42_RS19560 0.13 +0.1 4,227,855 + RR42_RS19560 0.15 -1.0 4,227,856 - RR42_RS19560 0.15 +3.2 4,227,895 - RR42_RS19560 0.21 +0.0 4,228,326 + +0.2 4,228,326 + +0.5 4,228,364 + -0.4 4,228,443 - -2.0 4,228,443 - +0.0 4,228,624 - RR42_RS19565 0.17 +0.6 4,228,624 - RR42_RS19565 0.17 -0.4 4,228,625 + RR42_RS19565 0.17 +0.2 4,228,625 + RR42_RS19565 0.17 +0.1 4,228,626 - RR42_RS19565 0.17 +0.2 4,228,626 - RR42_RS19565 0.17 +1.0 4,228,626 - RR42_RS19565 0.17 -0.7 4,228,626 - RR42_RS19565 0.17 +1.6
Or see this region's nucleotide sequence