Experiment: R2A with D-Cycloserine 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS18295 and RR42_RS18300 are separated by 24 nucleotides RR42_RS18300 and RR42_RS18305 are separated by 68 nucleotides RR42_RS18305 and RR42_RS18310 are separated by 35 nucleotides RR42_RS18310 and RR42_RS18315 are separated by 26 nucleotides
RR42_RS18295: RR42_RS18295 - anthranilate synthase component I, at 3,963,182 to 3,964,699
_RS18295
RR42_RS18300: RR42_RS18300 - anthranilate synthase subunit II, at 3,964,724 to 3,965,293
_RS18300
RR42_RS18305: RR42_RS18305 - anthranilate phosphoribosyltransferase, at 3,965,362 to 3,966,390
_RS18305
RR42_RS18310: RR42_RS18310 - indole-3-glycerol-phosphate synthase, at 3,966,426 to 3,967,229
_RS18310
RR42_RS18315: RR42_RS18315 - toxin, at 3,967,256 to 3,967,897
_RS18315
Position (kb)
3965
3966
3967 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3964.479 kb on - strand, within RR42_RS18295 at 3964.496 kb on + strand, within RR42_RS18295 at 3964.497 kb on - strand, within RR42_RS18295 at 3964.568 kb on + strand at 3964.568 kb on + strand at 3964.569 kb on - strand at 3964.569 kb on - strand at 3964.569 kb on - strand at 3964.569 kb on - strand at 3964.694 kb on - strand at 3964.697 kb on + strand at 3964.697 kb on + strand at 3964.697 kb on + strand at 3964.697 kb on + strand at 3964.697 kb on + strand at 3964.697 kb on + strand at 3964.697 kb on + strand at 3964.698 kb on - strand at 3964.698 kb on - strand at 3964.698 kb on - strand at 3964.698 kb on - strand at 3964.703 kb on - strand at 3964.745 kb on + strand at 3964.746 kb on - strand at 3964.746 kb on - strand at 3964.761 kb on - strand at 3964.861 kb on + strand, within RR42_RS18300 at 3964.861 kb on + strand, within RR42_RS18300 at 3964.861 kb on + strand, within RR42_RS18300 at 3964.861 kb on + strand, within RR42_RS18300 at 3964.861 kb on + strand, within RR42_RS18300 at 3964.862 kb on - strand, within RR42_RS18300 at 3964.862 kb on - strand, within RR42_RS18300 at 3965.103 kb on - strand, within RR42_RS18300 at 3965.901 kb on + strand, within RR42_RS18305 at 3965.902 kb on - strand, within RR42_RS18305 at 3965.902 kb on - strand, within RR42_RS18305 at 3966.028 kb on + strand, within RR42_RS18305 at 3966.388 kb on + strand at 3966.420 kb on + strand at 3966.687 kb on + strand, within RR42_RS18310 at 3966.831 kb on + strand, within RR42_RS18310 at 3966.832 kb on - strand, within RR42_RS18310 at 3967.240 kb on + strand at 3967.241 kb on - strand at 3967.241 kb on - strand at 3967.241 kb on - strand at 3967.354 kb on + strand, within RR42_RS18315 at 3967.354 kb on + strand, within RR42_RS18315 at 3967.354 kb on + strand, within RR42_RS18315 at 3967.355 kb on - strand, within RR42_RS18315 at 3967.355 kb on - strand, within RR42_RS18315 at 3967.355 kb on - strand, within RR42_RS18315 at 3967.355 kb on - strand, within RR42_RS18315 at 3967.371 kb on - strand, within RR42_RS18315 at 3967.371 kb on - strand, within RR42_RS18315
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.01 mg/ml remove 3,964,479 - RR42_RS18295 0.85 -1.1 3,964,496 + RR42_RS18295 0.87 +0.1 3,964,497 - RR42_RS18295 0.87 -1.2 3,964,568 + -0.2 3,964,568 + -0.1 3,964,569 - -0.5 3,964,569 - -1.3 3,964,569 - -0.1 3,964,569 - -0.5 3,964,694 - -0.1 3,964,697 + +0.4 3,964,697 + -0.1 3,964,697 + -0.7 3,964,697 + +0.2 3,964,697 + +0.4 3,964,697 + +1.0 3,964,697 + +0.4 3,964,698 - +0.0 3,964,698 - +1.3 3,964,698 - -0.5 3,964,698 - +0.8 3,964,703 - -0.3 3,964,745 + -0.6 3,964,746 - -0.8 3,964,746 - -1.2 3,964,761 - -2.4 3,964,861 + RR42_RS18300 0.24 -0.3 3,964,861 + RR42_RS18300 0.24 -0.3 3,964,861 + RR42_RS18300 0.24 -0.3 3,964,861 + RR42_RS18300 0.24 -1.1 3,964,861 + RR42_RS18300 0.24 -1.2 3,964,862 - RR42_RS18300 0.24 -1.0 3,964,862 - RR42_RS18300 0.24 -1.5 3,965,103 - RR42_RS18300 0.66 -0.2 3,965,901 + RR42_RS18305 0.52 -0.8 3,965,902 - RR42_RS18305 0.52 -0.3 3,965,902 - RR42_RS18305 0.52 -0.4 3,966,028 + RR42_RS18305 0.65 -0.5 3,966,388 + -0.8 3,966,420 + +0.1 3,966,687 + RR42_RS18310 0.32 +1.9 3,966,831 + RR42_RS18310 0.50 -1.5 3,966,832 - RR42_RS18310 0.50 +1.8 3,967,240 + +0.5 3,967,241 - +0.5 3,967,241 - +1.9 3,967,241 - +0.2 3,967,354 + RR42_RS18315 0.15 -0.5 3,967,354 + RR42_RS18315 0.15 +0.3 3,967,354 + RR42_RS18315 0.15 -0.3 3,967,355 - RR42_RS18315 0.15 -1.8 3,967,355 - RR42_RS18315 0.15 -0.2 3,967,355 - RR42_RS18315 0.15 -1.3 3,967,355 - RR42_RS18315 0.15 -1.4 3,967,371 - RR42_RS18315 0.18 -2.5 3,967,371 - RR42_RS18315 0.18 -0.4
Or see this region's nucleotide sequence