Experiment: R2A with D-Cycloserine 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS18295 and RR42_RS18300 are separated by 24 nucleotides RR42_RS18300 and RR42_RS18305 are separated by 68 nucleotides
RR42_RS18295: RR42_RS18295 - anthranilate synthase component I, at 3,963,182 to 3,964,699
_RS18295
RR42_RS18300: RR42_RS18300 - anthranilate synthase subunit II, at 3,964,724 to 3,965,293
_RS18300
RR42_RS18305: RR42_RS18305 - anthranilate phosphoribosyltransferase, at 3,965,362 to 3,966,390
_RS18305
Position (kb)
3964
3965
3966 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3963.827 kb on + strand, within RR42_RS18295 at 3963.851 kb on + strand, within RR42_RS18295 at 3963.881 kb on + strand, within RR42_RS18295 at 3963.881 kb on + strand, within RR42_RS18295 at 3963.882 kb on - strand, within RR42_RS18295 at 3963.882 kb on - strand, within RR42_RS18295 at 3963.882 kb on - strand, within RR42_RS18295 at 3963.962 kb on + strand, within RR42_RS18295 at 3963.995 kb on + strand, within RR42_RS18295 at 3963.995 kb on + strand, within RR42_RS18295 at 3963.995 kb on + strand, within RR42_RS18295 at 3963.996 kb on - strand, within RR42_RS18295 at 3963.996 kb on - strand, within RR42_RS18295 at 3963.996 kb on - strand, within RR42_RS18295 at 3963.996 kb on - strand, within RR42_RS18295 at 3963.996 kb on - strand, within RR42_RS18295 at 3964.001 kb on + strand, within RR42_RS18295 at 3964.001 kb on + strand, within RR42_RS18295 at 3964.001 kb on + strand, within RR42_RS18295 at 3964.001 kb on + strand, within RR42_RS18295 at 3964.002 kb on - strand, within RR42_RS18295 at 3964.002 kb on - strand, within RR42_RS18295 at 3964.002 kb on - strand, within RR42_RS18295 at 3964.002 kb on - strand, within RR42_RS18295 at 3964.002 kb on - strand, within RR42_RS18295 at 3964.007 kb on + strand, within RR42_RS18295 at 3964.310 kb on + strand, within RR42_RS18295 at 3964.310 kb on + strand, within RR42_RS18295 at 3964.310 kb on + strand, within RR42_RS18295 at 3964.310 kb on + strand, within RR42_RS18295 at 3964.311 kb on - strand, within RR42_RS18295 at 3964.479 kb on - strand, within RR42_RS18295 at 3964.496 kb on + strand, within RR42_RS18295 at 3964.497 kb on - strand, within RR42_RS18295 at 3964.568 kb on + strand at 3964.568 kb on + strand at 3964.569 kb on - strand at 3964.569 kb on - strand at 3964.569 kb on - strand at 3964.569 kb on - strand at 3964.694 kb on - strand at 3964.697 kb on + strand at 3964.697 kb on + strand at 3964.697 kb on + strand at 3964.697 kb on + strand at 3964.697 kb on + strand at 3964.697 kb on + strand at 3964.697 kb on + strand at 3964.698 kb on - strand at 3964.698 kb on - strand at 3964.698 kb on - strand at 3964.698 kb on - strand at 3964.703 kb on - strand at 3964.745 kb on + strand at 3964.746 kb on - strand at 3964.746 kb on - strand at 3964.761 kb on - strand at 3964.861 kb on + strand, within RR42_RS18300 at 3964.861 kb on + strand, within RR42_RS18300 at 3964.861 kb on + strand, within RR42_RS18300 at 3964.861 kb on + strand, within RR42_RS18300 at 3964.861 kb on + strand, within RR42_RS18300 at 3964.862 kb on - strand, within RR42_RS18300 at 3964.862 kb on - strand, within RR42_RS18300 at 3965.103 kb on - strand, within RR42_RS18300 at 3965.901 kb on + strand, within RR42_RS18305 at 3965.902 kb on - strand, within RR42_RS18305 at 3965.902 kb on - strand, within RR42_RS18305 at 3966.028 kb on + strand, within RR42_RS18305
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.01 mg/ml remove 3,963,827 + RR42_RS18295 0.42 +1.9 3,963,851 + RR42_RS18295 0.44 -0.8 3,963,881 + RR42_RS18295 0.46 +1.5 3,963,881 + RR42_RS18295 0.46 -0.1 3,963,882 - RR42_RS18295 0.46 -1.7 3,963,882 - RR42_RS18295 0.46 -1.1 3,963,882 - RR42_RS18295 0.46 +0.0 3,963,962 + RR42_RS18295 0.51 -0.7 3,963,995 + RR42_RS18295 0.54 -0.5 3,963,995 + RR42_RS18295 0.54 -1.1 3,963,995 + RR42_RS18295 0.54 -0.3 3,963,996 - RR42_RS18295 0.54 -1.0 3,963,996 - RR42_RS18295 0.54 +0.1 3,963,996 - RR42_RS18295 0.54 -1.9 3,963,996 - RR42_RS18295 0.54 -1.9 3,963,996 - RR42_RS18295 0.54 -0.7 3,964,001 + RR42_RS18295 0.54 +1.0 3,964,001 + RR42_RS18295 0.54 -0.3 3,964,001 + RR42_RS18295 0.54 +0.6 3,964,001 + RR42_RS18295 0.54 -0.1 3,964,002 - RR42_RS18295 0.54 -1.4 3,964,002 - RR42_RS18295 0.54 -0.2 3,964,002 - RR42_RS18295 0.54 -2.9 3,964,002 - RR42_RS18295 0.54 +0.5 3,964,002 - RR42_RS18295 0.54 -0.2 3,964,007 + RR42_RS18295 0.54 -2.0 3,964,310 + RR42_RS18295 0.74 -0.2 3,964,310 + RR42_RS18295 0.74 -0.1 3,964,310 + RR42_RS18295 0.74 -0.5 3,964,310 + RR42_RS18295 0.74 +0.1 3,964,311 - RR42_RS18295 0.74 +0.7 3,964,479 - RR42_RS18295 0.85 -1.1 3,964,496 + RR42_RS18295 0.87 +0.1 3,964,497 - RR42_RS18295 0.87 -1.2 3,964,568 + -0.2 3,964,568 + -0.1 3,964,569 - -0.5 3,964,569 - -1.3 3,964,569 - -0.1 3,964,569 - -0.5 3,964,694 - -0.1 3,964,697 + +0.4 3,964,697 + -0.1 3,964,697 + -0.7 3,964,697 + +0.2 3,964,697 + +0.4 3,964,697 + +1.0 3,964,697 + +0.4 3,964,698 - +0.0 3,964,698 - +1.3 3,964,698 - -0.5 3,964,698 - +0.8 3,964,703 - -0.3 3,964,745 + -0.6 3,964,746 - -0.8 3,964,746 - -1.2 3,964,761 - -2.4 3,964,861 + RR42_RS18300 0.24 -0.3 3,964,861 + RR42_RS18300 0.24 -0.3 3,964,861 + RR42_RS18300 0.24 -0.3 3,964,861 + RR42_RS18300 0.24 -1.1 3,964,861 + RR42_RS18300 0.24 -1.2 3,964,862 - RR42_RS18300 0.24 -1.0 3,964,862 - RR42_RS18300 0.24 -1.5 3,965,103 - RR42_RS18300 0.66 -0.2 3,965,901 + RR42_RS18305 0.52 -0.8 3,965,902 - RR42_RS18305 0.52 -0.3 3,965,902 - RR42_RS18305 0.52 -0.4 3,966,028 + RR42_RS18305 0.65 -0.5
Or see this region's nucleotide sequence