Experiment: R2A with D-Cycloserine 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS17710 and RR42_RS17715 are separated by 71 nucleotides RR42_RS17715 and RR42_RS17720 are separated by 115 nucleotides RR42_RS17720 and RR42_RS17725 are separated by 22 nucleotides RR42_RS17725 and RR42_RS17730 are separated by 507 nucleotides
RR42_RS17710: RR42_RS17710 - hypothetical protein, at 3,831,118 to 3,832,671
_RS17710
RR42_RS17715: RR42_RS17715 - thiol peroxidase, at 3,832,743 to 3,833,243
_RS17715
RR42_RS17720: RR42_RS17720 - sugar kinase, at 3,833,359 to 3,834,306
_RS17720
RR42_RS17725: RR42_RS17725 - membrane protein, at 3,834,329 to 3,834,796
_RS17725
RR42_RS17730: RR42_RS17730 - hypothetical protein, at 3,835,304 to 3,835,885
_RS17730
Position (kb)
3833
3834
3835 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 3832.421 kb on - strand, within RR42_RS17710 at 3832.443 kb on + strand, within RR42_RS17710 at 3832.543 kb on + strand at 3832.544 kb on - strand at 3832.663 kb on + strand at 3832.663 kb on + strand at 3832.664 kb on - strand at 3832.742 kb on + strand at 3832.742 kb on + strand at 3832.742 kb on + strand at 3832.742 kb on + strand at 3832.742 kb on + strand at 3832.742 kb on + strand at 3832.742 kb on + strand at 3832.742 kb on + strand at 3832.743 kb on - strand at 3832.743 kb on - strand at 3832.892 kb on + strand, within RR42_RS17715 at 3833.091 kb on + strand, within RR42_RS17715 at 3833.091 kb on + strand, within RR42_RS17715 at 3833.092 kb on - strand, within RR42_RS17715 at 3833.175 kb on + strand, within RR42_RS17715 at 3833.175 kb on + strand, within RR42_RS17715 at 3833.214 kb on + strand at 3833.214 kb on + strand at 3833.215 kb on - strand at 3833.242 kb on - strand at 3833.340 kb on + strand at 3833.340 kb on + strand at 3833.341 kb on - strand at 3833.929 kb on + strand, within RR42_RS17720 at 3833.930 kb on - strand, within RR42_RS17720 at 3834.071 kb on - strand, within RR42_RS17720 at 3834.243 kb on + strand at 3834.304 kb on + strand at 3834.328 kb on + strand at 3834.329 kb on - strand at 3834.422 kb on + strand, within RR42_RS17725 at 3834.422 kb on + strand, within RR42_RS17725 at 3834.422 kb on + strand, within RR42_RS17725 at 3834.422 kb on + strand, within RR42_RS17725 at 3834.422 kb on + strand, within RR42_RS17725 at 3834.422 kb on + strand, within RR42_RS17725 at 3834.422 kb on + strand, within RR42_RS17725 at 3834.423 kb on - strand, within RR42_RS17725 at 3834.423 kb on - strand, within RR42_RS17725 at 3834.424 kb on + strand, within RR42_RS17725 at 3834.425 kb on - strand, within RR42_RS17725 at 3834.425 kb on - strand, within RR42_RS17725 at 3834.425 kb on - strand, within RR42_RS17725 at 3834.795 kb on - strand at 3834.918 kb on + strand at 3835.212 kb on + strand at 3835.212 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.01 mg/ml remove 3,832,421 - RR42_RS17710 0.84 -0.7 3,832,443 + RR42_RS17710 0.85 +0.1 3,832,543 + -0.7 3,832,544 - -1.3 3,832,663 + -0.5 3,832,663 + +1.1 3,832,664 - -0.7 3,832,742 + -0.6 3,832,742 + -1.4 3,832,742 + -0.3 3,832,742 + -0.6 3,832,742 + +3.3 3,832,742 + +0.1 3,832,742 + +0.0 3,832,742 + -0.3 3,832,743 - -0.1 3,832,743 - -1.2 3,832,892 + RR42_RS17715 0.30 +0.8 3,833,091 + RR42_RS17715 0.69 +0.2 3,833,091 + RR42_RS17715 0.69 +1.3 3,833,092 - RR42_RS17715 0.70 +1.1 3,833,175 + RR42_RS17715 0.86 +0.1 3,833,175 + RR42_RS17715 0.86 -0.2 3,833,214 + +0.3 3,833,214 + -1.5 3,833,215 - -0.1 3,833,242 - -0.6 3,833,340 + -0.4 3,833,340 + +0.8 3,833,341 - -1.9 3,833,929 + RR42_RS17720 0.60 -0.9 3,833,930 - RR42_RS17720 0.60 -1.9 3,834,071 - RR42_RS17720 0.75 -0.1 3,834,243 + -0.9 3,834,304 + +0.0 3,834,328 + -0.3 3,834,329 - -1.3 3,834,422 + RR42_RS17725 0.20 -0.0 3,834,422 + RR42_RS17725 0.20 +0.3 3,834,422 + RR42_RS17725 0.20 -0.1 3,834,422 + RR42_RS17725 0.20 +0.3 3,834,422 + RR42_RS17725 0.20 +0.4 3,834,422 + RR42_RS17725 0.20 +0.4 3,834,422 + RR42_RS17725 0.20 -0.1 3,834,423 - RR42_RS17725 0.20 +1.1 3,834,423 - RR42_RS17725 0.20 +1.3 3,834,424 + RR42_RS17725 0.20 +1.1 3,834,425 - RR42_RS17725 0.21 -0.1 3,834,425 - RR42_RS17725 0.21 +0.6 3,834,425 - RR42_RS17725 0.21 -0.1 3,834,795 - -0.4 3,834,918 + -0.6 3,835,212 + +0.5 3,835,212 + -1.3
Or see this region's nucleotide sequence