Experiment: R2A with D-Cycloserine 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS16920 and RR42_RS16925 are separated by 76 nucleotides RR42_RS16925 and RR42_RS16930 are separated by 15 nucleotides RR42_RS16930 and RR42_RS16935 overlap by 4 nucleotides RR42_RS16935 and RR42_RS16940 are separated by 13 nucleotides
RR42_RS16920: RR42_RS16920 - histidine ammonia-lyase, at 3,662,144 to 3,663,724
_RS16920
RR42_RS16925: RR42_RS16925 - histidine utilization repressor, at 3,663,801 to 3,664,574
_RS16925
RR42_RS16930: RR42_RS16930 - sulfonate ABC transporter ATP-binding protein, at 3,664,590 to 3,665,369
_RS16930
RR42_RS16935: RR42_RS16935 - ABC transporter permease, at 3,665,366 to 3,666,238
_RS16935
RR42_RS16940: RR42_RS16940 - ABC transporter permease, at 3,666,252 to 3,667,223
_RS16940
Position (kb)
3664
3665
3666 Strain fitness (log2 ratio)
-1
0
1
2 at 3663.876 kb on + strand at 3663.877 kb on - strand at 3663.877 kb on - strand at 3663.877 kb on - strand at 3663.877 kb on - strand at 3663.877 kb on - strand at 3663.877 kb on - strand at 3664.013 kb on - strand, within RR42_RS16925 at 3664.033 kb on + strand, within RR42_RS16925 at 3664.108 kb on + strand, within RR42_RS16925 at 3664.109 kb on - strand, within RR42_RS16925 at 3664.109 kb on - strand, within RR42_RS16925 at 3664.252 kb on + strand, within RR42_RS16925 at 3664.252 kb on + strand, within RR42_RS16925 at 3664.253 kb on - strand, within RR42_RS16925 at 3664.253 kb on - strand, within RR42_RS16925 at 3664.498 kb on + strand at 3664.498 kb on + strand at 3664.499 kb on - strand at 3664.516 kb on + strand at 3664.517 kb on - strand at 3664.681 kb on + strand, within RR42_RS16930 at 3664.681 kb on + strand, within RR42_RS16930 at 3664.682 kb on - strand, within RR42_RS16930 at 3665.113 kb on + strand, within RR42_RS16930 at 3665.113 kb on + strand, within RR42_RS16930 at 3665.114 kb on - strand, within RR42_RS16930 at 3665.506 kb on + strand, within RR42_RS16935 at 3665.506 kb on + strand, within RR42_RS16935 at 3665.506 kb on + strand, within RR42_RS16935 at 3665.506 kb on + strand, within RR42_RS16935 at 3665.507 kb on - strand, within RR42_RS16935 at 3665.507 kb on - strand, within RR42_RS16935 at 3665.553 kb on + strand, within RR42_RS16935 at 3665.553 kb on + strand, within RR42_RS16935 at 3665.554 kb on - strand, within RR42_RS16935 at 3665.554 kb on - strand, within RR42_RS16935 at 3665.554 kb on - strand, within RR42_RS16935 at 3665.554 kb on - strand, within RR42_RS16935 at 3665.554 kb on - strand, within RR42_RS16935 at 3665.554 kb on - strand, within RR42_RS16935 at 3665.661 kb on + strand, within RR42_RS16935 at 3665.662 kb on - strand, within RR42_RS16935 at 3665.820 kb on + strand, within RR42_RS16935 at 3665.869 kb on - strand, within RR42_RS16935 at 3665.939 kb on + strand, within RR42_RS16935 at 3665.940 kb on - strand, within RR42_RS16935 at 3665.971 kb on - strand, within RR42_RS16935 at 3665.981 kb on + strand, within RR42_RS16935 at 3665.981 kb on + strand, within RR42_RS16935 at 3665.981 kb on + strand, within RR42_RS16935 at 3665.981 kb on + strand, within RR42_RS16935 at 3665.982 kb on - strand, within RR42_RS16935 at 3665.982 kb on - strand, within RR42_RS16935 at 3665.982 kb on - strand, within RR42_RS16935 at 3666.253 kb on + strand at 3666.253 kb on + strand at 3666.254 kb on - strand at 3666.275 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.01 mg/ml remove 3,663,876 + -0.5 3,663,877 - +0.2 3,663,877 - +1.0 3,663,877 - -0.5 3,663,877 - -0.3 3,663,877 - +0.3 3,663,877 - +0.4 3,664,013 - RR42_RS16925 0.27 +1.1 3,664,033 + RR42_RS16925 0.30 -0.3 3,664,108 + RR42_RS16925 0.40 -1.1 3,664,109 - RR42_RS16925 0.40 -0.5 3,664,109 - RR42_RS16925 0.40 -0.0 3,664,252 + RR42_RS16925 0.58 +0.7 3,664,252 + RR42_RS16925 0.58 -0.2 3,664,253 - RR42_RS16925 0.58 -1.0 3,664,253 - RR42_RS16925 0.58 +0.4 3,664,498 + -0.3 3,664,498 + +0.4 3,664,499 - +0.3 3,664,516 + +0.3 3,664,517 - +0.0 3,664,681 + RR42_RS16930 0.12 -0.9 3,664,681 + RR42_RS16930 0.12 +0.3 3,664,682 - RR42_RS16930 0.12 +0.9 3,665,113 + RR42_RS16930 0.67 +1.6 3,665,113 + RR42_RS16930 0.67 +1.2 3,665,114 - RR42_RS16930 0.67 +0.7 3,665,506 + RR42_RS16935 0.16 -0.1 3,665,506 + RR42_RS16935 0.16 -0.7 3,665,506 + RR42_RS16935 0.16 -0.9 3,665,506 + RR42_RS16935 0.16 -0.8 3,665,507 - RR42_RS16935 0.16 -0.3 3,665,507 - RR42_RS16935 0.16 +0.1 3,665,553 + RR42_RS16935 0.21 +2.4 3,665,553 + RR42_RS16935 0.21 -0.9 3,665,554 - RR42_RS16935 0.22 -0.3 3,665,554 - RR42_RS16935 0.22 +0.4 3,665,554 - RR42_RS16935 0.22 +0.3 3,665,554 - RR42_RS16935 0.22 +0.5 3,665,554 - RR42_RS16935 0.22 +0.5 3,665,554 - RR42_RS16935 0.22 +0.5 3,665,661 + RR42_RS16935 0.34 +0.2 3,665,662 - RR42_RS16935 0.34 +1.2 3,665,820 + RR42_RS16935 0.52 +0.0 3,665,869 - RR42_RS16935 0.58 -0.5 3,665,939 + RR42_RS16935 0.66 +0.0 3,665,940 - RR42_RS16935 0.66 +0.0 3,665,971 - RR42_RS16935 0.69 +0.2 3,665,981 + RR42_RS16935 0.70 -0.4 3,665,981 + RR42_RS16935 0.70 -0.8 3,665,981 + RR42_RS16935 0.70 +0.3 3,665,981 + RR42_RS16935 0.70 +0.7 3,665,982 - RR42_RS16935 0.71 -0.8 3,665,982 - RR42_RS16935 0.71 +0.5 3,665,982 - RR42_RS16935 0.71 -0.2 3,666,253 + -0.8 3,666,253 + -0.1 3,666,254 - +0.1 3,666,275 - +1.8
Or see this region's nucleotide sequence