Strain Fitness in Cupriavidus basilensis FW507-4G11 around RR42_RS15595

Experiment: R2A with D-Cycloserine 0.01 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRR42_RS15590 and RR42_RS15595 are separated by 82 nucleotidesRR42_RS15595 and RR42_RS15600 are separated by 76 nucleotides RR42_RS15590: RR42_RS15590 - 3-octaprenyl-4-hydroxybenzoate carboxy-lyase, at 3,366,920 to 3,368,449 _RS15590 RR42_RS15595: RR42_RS15595 - acetoacetyl-CoA synthetase, at 3,368,532 to 3,370,568 _RS15595 RR42_RS15600: RR42_RS15600 - glutathione S-transferase, at 3,370,645 to 3,371,301 _RS15600 Position (kb) 3368 3369 3370 3371Strain fitness (log2 ratio) -3 -2 -1 0 1at 3368.464 kb on + strandat 3368.464 kb on + strandat 3368.465 kb on - strandat 3368.715 kb on + strandat 3368.808 kb on + strand, within RR42_RS15595at 3368.808 kb on + strand, within RR42_RS15595at 3368.809 kb on - strand, within RR42_RS15595at 3368.809 kb on - strand, within RR42_RS15595at 3368.809 kb on - strand, within RR42_RS15595at 3368.992 kb on - strand, within RR42_RS15595at 3369.473 kb on + strand, within RR42_RS15595at 3369.504 kb on + strand, within RR42_RS15595at 3369.504 kb on + strand, within RR42_RS15595at 3369.505 kb on - strand, within RR42_RS15595at 3369.577 kb on - strand, within RR42_RS15595at 3369.719 kb on - strand, within RR42_RS15595at 3369.771 kb on + strand, within RR42_RS15595at 3369.771 kb on + strand, within RR42_RS15595at 3369.772 kb on - strand, within RR42_RS15595at 3369.879 kb on + strand, within RR42_RS15595at 3370.122 kb on + strand, within RR42_RS15595at 3370.123 kb on - strand, within RR42_RS15595at 3370.240 kb on - strand, within RR42_RS15595at 3370.240 kb on - strand, within RR42_RS15595at 3370.240 kb on - strand, within RR42_RS15595at 3370.257 kb on + strand, within RR42_RS15595at 3370.257 kb on + strand, within RR42_RS15595at 3370.258 kb on - strand, within RR42_RS15595at 3370.258 kb on - strand, within RR42_RS15595at 3370.258 kb on - strand, within RR42_RS15595at 3370.258 kb on - strand, within RR42_RS15595at 3370.259 kb on + strand, within RR42_RS15595at 3370.260 kb on - strand, within RR42_RS15595at 3370.260 kb on - strand, within RR42_RS15595at 3370.509 kb on + strandat 3370.510 kb on - strandat 3370.742 kb on + strand, within RR42_RS15600at 3370.775 kb on + strand, within RR42_RS15600at 3370.775 kb on + strand, within RR42_RS15600at 3370.776 kb on - strand, within RR42_RS15600at 3370.776 kb on - strand, within RR42_RS15600at 3370.806 kb on - strand, within RR42_RS15600at 3370.806 kb on - strand, within RR42_RS15600at 3370.845 kb on - strand, within RR42_RS15600at 3371.079 kb on - strand, within RR42_RS15600at 3371.272 kb on - strandat 3371.435 kb on + strandat 3371.436 kb on - strandat 3371.449 kb on - strandat 3371.451 kb on + strandat 3371.451 kb on + strandat 3371.451 kb on + strandat 3371.497 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.01 mg/ml
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3,368,464 + -0.4
3,368,464 + -0.6
3,368,465 - -0.4
3,368,715 + -0.2
3,368,808 + RR42_RS15595 0.14 -2.8
3,368,808 + RR42_RS15595 0.14 +0.1
3,368,809 - RR42_RS15595 0.14 -0.2
3,368,809 - RR42_RS15595 0.14 -0.3
3,368,809 - RR42_RS15595 0.14 -1.3
3,368,992 - RR42_RS15595 0.23 +0.1
3,369,473 + RR42_RS15595 0.46 -0.2
3,369,504 + RR42_RS15595 0.48 +0.6
3,369,504 + RR42_RS15595 0.48 -0.1
3,369,505 - RR42_RS15595 0.48 -0.8
3,369,577 - RR42_RS15595 0.51 -1.5
3,369,719 - RR42_RS15595 0.58 -0.2
3,369,771 + RR42_RS15595 0.61 +0.2
3,369,771 + RR42_RS15595 0.61 -1.1
3,369,772 - RR42_RS15595 0.61 -0.8
3,369,879 + RR42_RS15595 0.66 +0.5
3,370,122 + RR42_RS15595 0.78 +1.3
3,370,123 - RR42_RS15595 0.78 -0.3
3,370,240 - RR42_RS15595 0.84 -0.4
3,370,240 - RR42_RS15595 0.84 +0.4
3,370,240 - RR42_RS15595 0.84 -0.5
3,370,257 + RR42_RS15595 0.85 +0.0
3,370,257 + RR42_RS15595 0.85 +0.1
3,370,258 - RR42_RS15595 0.85 -0.1
3,370,258 - RR42_RS15595 0.85 -0.2
3,370,258 - RR42_RS15595 0.85 -0.3
3,370,258 - RR42_RS15595 0.85 -0.5
3,370,259 + RR42_RS15595 0.85 +0.0
3,370,260 - RR42_RS15595 0.85 -0.2
3,370,260 - RR42_RS15595 0.85 -0.3
3,370,509 + +1.0
3,370,510 - -0.7
3,370,742 + RR42_RS15600 0.15 -0.3
3,370,775 + RR42_RS15600 0.20 +0.3
3,370,775 + RR42_RS15600 0.20 -2.3
3,370,776 - RR42_RS15600 0.20 -2.0
3,370,776 - RR42_RS15600 0.20 -0.6
3,370,806 - RR42_RS15600 0.25 -1.3
3,370,806 - RR42_RS15600 0.25 +0.3
3,370,845 - RR42_RS15600 0.30 +0.5
3,371,079 - RR42_RS15600 0.66 -0.0
3,371,272 - -1.1
3,371,435 + -0.4
3,371,436 - +1.6
3,371,449 - +0.6
3,371,451 + +0.5
3,371,451 + +0.3
3,371,451 + +0.4
3,371,497 + +0.3

Or see this region's nucleotide sequence