Experiment: R2A with D-Cycloserine 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS10945 and RR42_RS10950 are separated by 19 nucleotides RR42_RS10950 and RR42_RS10955 are separated by 209 nucleotides RR42_RS10955 and RR42_RS10960 are separated by 41 nucleotides RR42_RS10960 and RR42_RS10965 are separated by 11 nucleotides
RR42_RS10945: RR42_RS10945 - MFS transporter, at 2,338,565 to 2,339,650
_RS10945
RR42_RS10950: RR42_RS10950 - GCN5 family acetyltransferase, at 2,339,670 to 2,340,095
_RS10950
RR42_RS10955: RR42_RS10955 - GntR family transcriptional regulator, at 2,340,305 to 2,341,057
_RS10955
RR42_RS10960: RR42_RS10960 - 3-hydroxyisobutyrate dehydrogenase, at 2,341,099 to 2,341,995
_RS10960
RR42_RS10965: RR42_RS10965 - membrane protein, at 2,342,007 to 2,343,311
_RS10965
Position (kb)
2340
2341
2342 Strain fitness (log2 ratio)
-1
0
1
2
3 at 2339.506 kb on + strand, within RR42_RS10945 at 2339.506 kb on + strand, within RR42_RS10945 at 2339.506 kb on + strand, within RR42_RS10945 at 2339.506 kb on + strand, within RR42_RS10945 at 2339.506 kb on + strand, within RR42_RS10945 at 2339.506 kb on + strand, within RR42_RS10945 at 2339.507 kb on - strand, within RR42_RS10945 at 2339.507 kb on - strand, within RR42_RS10945 at 2339.567 kb on + strand at 2339.568 kb on - strand at 2339.610 kb on + strand at 2339.755 kb on + strand, within RR42_RS10950 at 2339.755 kb on + strand, within RR42_RS10950 at 2339.755 kb on + strand, within RR42_RS10950 at 2339.770 kb on + strand, within RR42_RS10950 at 2340.157 kb on + strand at 2340.234 kb on + strand at 2340.279 kb on + strand at 2340.282 kb on + strand at 2340.373 kb on - strand at 2340.418 kb on - strand, within RR42_RS10955 at 2340.560 kb on + strand, within RR42_RS10955 at 2340.560 kb on + strand, within RR42_RS10955 at 2340.561 kb on - strand, within RR42_RS10955 at 2340.818 kb on + strand, within RR42_RS10955 at 2340.819 kb on - strand, within RR42_RS10955 at 2340.847 kb on + strand, within RR42_RS10955 at 2340.964 kb on + strand, within RR42_RS10955 at 2340.964 kb on + strand, within RR42_RS10955 at 2340.965 kb on - strand, within RR42_RS10955 at 2340.965 kb on - strand, within RR42_RS10955 at 2340.965 kb on - strand, within RR42_RS10955 at 2340.965 kb on - strand, within RR42_RS10955 at 2340.965 kb on - strand, within RR42_RS10955 at 2340.965 kb on - strand, within RR42_RS10955 at 2340.965 kb on - strand, within RR42_RS10955 at 2340.965 kb on - strand, within RR42_RS10955 at 2340.965 kb on - strand, within RR42_RS10955 at 2340.965 kb on - strand, within RR42_RS10955 at 2340.968 kb on + strand, within RR42_RS10955 at 2340.968 kb on + strand, within RR42_RS10955 at 2340.968 kb on + strand, within RR42_RS10955 at 2340.968 kb on + strand, within RR42_RS10955 at 2340.968 kb on + strand, within RR42_RS10955 at 2340.969 kb on - strand, within RR42_RS10955 at 2340.969 kb on - strand, within RR42_RS10955 at 2340.969 kb on - strand, within RR42_RS10955 at 2341.181 kb on + strand at 2341.181 kb on + strand at 2341.181 kb on + strand at 2341.181 kb on + strand at 2341.181 kb on + strand at 2341.182 kb on - strand at 2341.182 kb on - strand at 2341.532 kb on - strand, within RR42_RS10960 at 2341.532 kb on - strand, within RR42_RS10960 at 2341.532 kb on - strand, within RR42_RS10960 at 2341.576 kb on + strand, within RR42_RS10960 at 2341.648 kb on - strand, within RR42_RS10960 at 2341.711 kb on + strand, within RR42_RS10960 at 2341.711 kb on + strand, within RR42_RS10960 at 2341.712 kb on - strand, within RR42_RS10960 at 2341.770 kb on + strand, within RR42_RS10960 at 2341.771 kb on - strand, within RR42_RS10960 at 2341.786 kb on + strand, within RR42_RS10960 at 2341.786 kb on + strand, within RR42_RS10960 at 2341.809 kb on + strand, within RR42_RS10960 at 2341.955 kb on + strand at 2341.955 kb on + strand at 2341.956 kb on - strand at 2341.956 kb on - strand at 2341.956 kb on - strand at 2341.956 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.01 mg/ml remove 2,339,506 + RR42_RS10945 0.87 -0.9 2,339,506 + RR42_RS10945 0.87 +0.2 2,339,506 + RR42_RS10945 0.87 +0.2 2,339,506 + RR42_RS10945 0.87 -0.3 2,339,506 + RR42_RS10945 0.87 -0.5 2,339,506 + RR42_RS10945 0.87 +0.2 2,339,507 - RR42_RS10945 0.87 +1.1 2,339,507 - RR42_RS10945 0.87 +0.1 2,339,567 + +1.2 2,339,568 - +0.4 2,339,610 + -0.5 2,339,755 + RR42_RS10950 0.20 -1.5 2,339,755 + RR42_RS10950 0.20 +0.2 2,339,755 + RR42_RS10950 0.20 +0.9 2,339,770 + RR42_RS10950 0.23 +1.4 2,340,157 + +0.5 2,340,234 + -0.7 2,340,279 + +0.1 2,340,282 + -1.5 2,340,373 - +0.0 2,340,418 - RR42_RS10955 0.15 +0.3 2,340,560 + RR42_RS10955 0.34 -0.5 2,340,560 + RR42_RS10955 0.34 -0.1 2,340,561 - RR42_RS10955 0.34 +1.1 2,340,818 + RR42_RS10955 0.68 +0.3 2,340,819 - RR42_RS10955 0.68 -0.5 2,340,847 + RR42_RS10955 0.72 +0.3 2,340,964 + RR42_RS10955 0.88 +0.1 2,340,964 + RR42_RS10955 0.88 +0.2 2,340,965 - RR42_RS10955 0.88 +0.8 2,340,965 - RR42_RS10955 0.88 +0.5 2,340,965 - RR42_RS10955 0.88 +0.5 2,340,965 - RR42_RS10955 0.88 +0.7 2,340,965 - RR42_RS10955 0.88 -0.3 2,340,965 - RR42_RS10955 0.88 +0.5 2,340,965 - RR42_RS10955 0.88 -0.2 2,340,965 - RR42_RS10955 0.88 +1.0 2,340,965 - RR42_RS10955 0.88 +0.7 2,340,965 - RR42_RS10955 0.88 +0.1 2,340,968 + RR42_RS10955 0.88 -1.2 2,340,968 + RR42_RS10955 0.88 -0.5 2,340,968 + RR42_RS10955 0.88 +0.3 2,340,968 + RR42_RS10955 0.88 -0.4 2,340,968 + RR42_RS10955 0.88 +0.1 2,340,969 - RR42_RS10955 0.88 -0.0 2,340,969 - RR42_RS10955 0.88 -0.1 2,340,969 - RR42_RS10955 0.88 -1.5 2,341,181 + -0.5 2,341,181 + -0.1 2,341,181 + -0.0 2,341,181 + -0.2 2,341,181 + +0.6 2,341,182 - -0.7 2,341,182 - -0.0 2,341,532 - RR42_RS10960 0.48 -0.2 2,341,532 - RR42_RS10960 0.48 +0.8 2,341,532 - RR42_RS10960 0.48 +0.1 2,341,576 + RR42_RS10960 0.53 -0.2 2,341,648 - RR42_RS10960 0.61 +2.5 2,341,711 + RR42_RS10960 0.68 -0.1 2,341,711 + RR42_RS10960 0.68 +0.2 2,341,712 - RR42_RS10960 0.68 +0.1 2,341,770 + RR42_RS10960 0.75 -0.0 2,341,771 - RR42_RS10960 0.75 -0.2 2,341,786 + RR42_RS10960 0.77 +1.0 2,341,786 + RR42_RS10960 0.77 +3.3 2,341,809 + RR42_RS10960 0.79 -0.3 2,341,955 + -0.8 2,341,955 + -0.1 2,341,956 - +0.7 2,341,956 - +0.2 2,341,956 - -1.4 2,341,956 - -0.5
Or see this region's nucleotide sequence