Experiment: R2A with D-Cycloserine 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS10500 and RR42_RS10505 are separated by 667 nucleotides RR42_RS10505 and RR42_RS10510 are separated by 107 nucleotides
RR42_RS10500: RR42_RS10500 - hypothetical protein, at 2,244,981 to 2,245,187
_RS10500
RR42_RS10505: RR42_RS10505 - citrate synthase, at 2,245,855 to 2,246,997
_RS10505
RR42_RS10510: RR42_RS10510 - dihydrofolate reductase, at 2,247,105 to 2,248,058
_RS10510
Position (kb)
2245
2246
2247 Strain fitness (log2 ratio)
-1
0
1
2 at 2244.982 kb on + strand at 2244.982 kb on + strand at 2244.988 kb on + strand at 2245.137 kb on + strand, within RR42_RS10500 at 2245.149 kb on + strand, within RR42_RS10500 at 2245.149 kb on + strand, within RR42_RS10500 at 2245.150 kb on - strand, within RR42_RS10500 at 2245.449 kb on + strand at 2245.449 kb on + strand at 2245.449 kb on + strand at 2245.449 kb on + strand at 2245.449 kb on + strand at 2245.449 kb on + strand at 2245.449 kb on + strand at 2245.449 kb on + strand at 2245.450 kb on - strand at 2245.450 kb on - strand at 2245.450 kb on - strand at 2245.472 kb on - strand at 2245.520 kb on + strand at 2245.521 kb on - strand at 2245.529 kb on + strand at 2245.530 kb on - strand at 2245.530 kb on - strand at 2245.530 kb on - strand at 2245.530 kb on - strand at 2245.535 kb on + strand at 2245.574 kb on + strand at 2245.574 kb on + strand at 2245.574 kb on + strand at 2245.574 kb on + strand at 2245.574 kb on + strand at 2245.575 kb on - strand at 2245.575 kb on - strand at 2245.575 kb on - strand at 2245.575 kb on - strand at 2245.654 kb on + strand at 2245.655 kb on - strand at 2245.655 kb on - strand at 2245.665 kb on + strand at 2245.666 kb on - strand at 2245.666 kb on - strand at 2245.686 kb on + strand at 2245.686 kb on + strand at 2245.687 kb on - strand at 2245.687 kb on - strand at 2245.687 kb on - strand at 2245.731 kb on + strand at 2245.732 kb on - strand at 2245.857 kb on - strand at 2245.883 kb on + strand at 2245.932 kb on + strand at 2245.932 kb on + strand at 2245.932 kb on + strand at 2246.000 kb on + strand, within RR42_RS10505 at 2246.000 kb on + strand, within RR42_RS10505 at 2246.000 kb on + strand, within RR42_RS10505 at 2246.001 kb on - strand, within RR42_RS10505 at 2246.001 kb on - strand, within RR42_RS10505 at 2246.144 kb on + strand, within RR42_RS10505 at 2246.144 kb on + strand, within RR42_RS10505 at 2246.144 kb on + strand, within RR42_RS10505 at 2246.729 kb on + strand, within RR42_RS10505 at 2246.730 kb on - strand, within RR42_RS10505 at 2246.923 kb on + strand at 2246.923 kb on + strand at 2246.924 kb on - strand at 2246.924 kb on - strand at 2246.927 kb on + strand at 2246.927 kb on + strand at 2246.927 kb on + strand at 2246.927 kb on + strand at 2246.927 kb on + strand at 2246.927 kb on + strand at 2246.927 kb on + strand at 2246.927 kb on + strand at 2246.928 kb on - strand at 2246.928 kb on - strand at 2246.928 kb on - strand at 2246.933 kb on + strand at 2247.067 kb on + strand at 2247.068 kb on - strand at 2247.174 kb on + strand at 2247.175 kb on - strand at 2247.175 kb on - strand at 2247.190 kb on - strand at 2247.266 kb on + strand, within RR42_RS10510 at 2247.358 kb on - strand, within RR42_RS10510 at 2247.358 kb on - strand, within RR42_RS10510 at 2247.631 kb on - strand, within RR42_RS10510 at 2247.632 kb on + strand, within RR42_RS10510 at 2247.633 kb on - strand, within RR42_RS10510
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.01 mg/ml remove 2,244,982 + +1.4 2,244,982 + -0.4 2,244,988 + -0.4 2,245,137 + RR42_RS10500 0.75 -0.1 2,245,149 + RR42_RS10500 0.81 +0.4 2,245,149 + RR42_RS10500 0.81 -0.4 2,245,150 - RR42_RS10500 0.82 +0.1 2,245,449 + +0.1 2,245,449 + -0.3 2,245,449 + +0.0 2,245,449 + +0.1 2,245,449 + +2.0 2,245,449 + -0.4 2,245,449 + +0.3 2,245,449 + +0.4 2,245,450 - -0.5 2,245,450 - +0.4 2,245,450 - +0.5 2,245,472 - +0.9 2,245,520 + -1.4 2,245,521 - +0.4 2,245,529 + -0.8 2,245,530 - +0.6 2,245,530 - +0.3 2,245,530 - +0.3 2,245,530 - -0.5 2,245,535 + -0.4 2,245,574 + +0.3 2,245,574 + -0.0 2,245,574 + +0.1 2,245,574 + -0.4 2,245,574 + +0.1 2,245,575 - +0.2 2,245,575 - +1.1 2,245,575 - -0.3 2,245,575 - +0.5 2,245,654 + +0.2 2,245,655 - +0.6 2,245,655 - -0.1 2,245,665 + +1.4 2,245,666 - -0.1 2,245,666 - +0.4 2,245,686 + +0.1 2,245,686 + -0.0 2,245,687 - +1.2 2,245,687 - -0.6 2,245,687 - -1.2 2,245,731 + -0.3 2,245,732 - -0.2 2,245,857 - -1.4 2,245,883 + -0.6 2,245,932 + +1.3 2,245,932 + -0.3 2,245,932 + -0.2 2,246,000 + RR42_RS10505 0.13 +0.5 2,246,000 + RR42_RS10505 0.13 +0.9 2,246,000 + RR42_RS10505 0.13 -0.1 2,246,001 - RR42_RS10505 0.13 +0.7 2,246,001 - RR42_RS10505 0.13 +0.9 2,246,144 + RR42_RS10505 0.25 -0.3 2,246,144 + RR42_RS10505 0.25 +0.5 2,246,144 + RR42_RS10505 0.25 +0.4 2,246,729 + RR42_RS10505 0.76 -0.2 2,246,730 - RR42_RS10505 0.77 +0.3 2,246,923 + +0.2 2,246,923 + -0.0 2,246,924 - -0.1 2,246,924 - -0.1 2,246,927 + -0.6 2,246,927 + -0.7 2,246,927 + -1.0 2,246,927 + -0.0 2,246,927 + -0.2 2,246,927 + -1.1 2,246,927 + -0.1 2,246,927 + +0.7 2,246,928 - +0.4 2,246,928 - +0.5 2,246,928 - -0.7 2,246,933 + -0.3 2,247,067 + +0.6 2,247,068 - +0.6 2,247,174 + +1.3 2,247,175 - -0.1 2,247,175 - -0.1 2,247,190 - -0.3 2,247,266 + RR42_RS10510 0.17 -0.7 2,247,358 - RR42_RS10510 0.27 -0.9 2,247,358 - RR42_RS10510 0.27 +0.1 2,247,631 - RR42_RS10510 0.55 -0.1 2,247,632 + RR42_RS10510 0.55 -0.0 2,247,633 - RR42_RS10510 0.55 +0.8
Or see this region's nucleotide sequence