Experiment: R2A with D-Cycloserine 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS08165 and RR42_RS08170 overlap by 4 nucleotides RR42_RS08170 and RR42_RS08175 overlap by 4 nucleotides RR42_RS08175 and RR42_RS08180 overlap by 4 nucleotides RR42_RS08180 and RR42_RS08185 overlap by 4 nucleotides RR42_RS08185 and RR42_RS08190 are separated by 25 nucleotides
RR42_RS08165: RR42_RS08165 - hypothetical protein, at 1,739,231 to 1,739,686
_RS08165
RR42_RS08170: RR42_RS08170 - hypothetical protein, at 1,739,683 to 1,740,042
_RS08170
RR42_RS08175: RR42_RS08175 - hypothetical protein, at 1,740,039 to 1,740,311
_RS08175
RR42_RS08180: RR42_RS08180 - hypothetical protein, at 1,740,308 to 1,740,577
_RS08180
RR42_RS08185: RR42_RS08185 - hypothetical protein, at 1,740,574 to 1,741,428
_RS08185
RR42_RS08190: RR42_RS08190 - hypothetical protein, at 1,741,454 to 1,741,774
_RS08190
Position (kb) 1740
1741 Strain fitness (log2 ratio) -1
0
1
2 at 1739.484 kb on - strand, within RR42_RS08165 at 1739.484 kb on - strand, within RR42_RS08165 at 1739.484 kb on - strand, within RR42_RS08165 at 1739.628 kb on - strand, within RR42_RS08165 at 1739.771 kb on - strand, within RR42_RS08170 at 1739.792 kb on - strand, within RR42_RS08170 at 1739.886 kb on - strand, within RR42_RS08170 at 1739.886 kb on - strand, within RR42_RS08170 at 1740.067 kb on - strand, within RR42_RS08175 at 1740.322 kb on - strand at 1740.322 kb on - strand at 1740.459 kb on - strand, within RR42_RS08180 at 1740.571 kb on - strand at 1740.617 kb on - strand at 1740.695 kb on - strand, within RR42_RS08185 at 1740.695 kb on - strand, within RR42_RS08185 at 1740.800 kb on - strand, within RR42_RS08185 at 1741.371 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.01 mg/ml remove 1,739,484 - RR42_RS08165 0.55 -1.2 1,739,484 - RR42_RS08165 0.55 +2.0 1,739,484 - RR42_RS08165 0.55 -0.1 1,739,628 - RR42_RS08165 0.87 +0.6 1,739,771 - RR42_RS08170 0.24 +0.7 1,739,792 - RR42_RS08170 0.30 -0.5 1,739,886 - RR42_RS08170 0.56 +2.4 1,739,886 - RR42_RS08170 0.56 -0.8 1,740,067 - RR42_RS08175 0.10 +0.3 1,740,322 - -0.3 1,740,322 - -0.1 1,740,459 - RR42_RS08180 0.56 -0.7 1,740,571 - +0.2 1,740,617 - +0.3 1,740,695 - RR42_RS08185 0.14 -0.2 1,740,695 - RR42_RS08185 0.14 +1.4 1,740,800 - RR42_RS08185 0.26 -1.1 1,741,371 - +0.4
Or see this region's nucleotide sequence