Experiment: R2A with D-Cycloserine 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS07615 and RR42_RS07620 are separated by 396 nucleotides RR42_RS07620 and RR42_RS07625 are separated by 362 nucleotides
RR42_RS07615: RR42_RS07615 - 3-ketoacyl-ACP reductase, at 1,631,470 to 1,632,210
_RS07615
RR42_RS07620: RR42_RS07620 - polyhydroxyalkanoate synthesis repressor PhaR, at 1,632,607 to 1,633,158
_RS07620
RR42_RS07625: RR42_RS07625 - dihydrouridine synthase, at 1,633,521 to 1,634,501
_RS07625
Position (kb)
1632
1633
1634 Strain fitness (log2 ratio)
-1
0
1 at 1631.926 kb on + strand, within RR42_RS07615 at 1631.926 kb on + strand, within RR42_RS07615 at 1631.927 kb on - strand, within RR42_RS07615 at 1632.324 kb on + strand at 1632.401 kb on + strand at 1632.401 kb on + strand at 1632.442 kb on + strand at 1632.492 kb on + strand at 1632.492 kb on + strand at 1632.492 kb on + strand at 1632.493 kb on - strand at 1632.541 kb on + strand at 1632.541 kb on + strand at 1632.667 kb on + strand, within RR42_RS07620 at 1632.688 kb on + strand, within RR42_RS07620 at 1632.689 kb on - strand, within RR42_RS07620 at 1632.866 kb on - strand, within RR42_RS07620 at 1632.898 kb on + strand, within RR42_RS07620 at 1632.898 kb on + strand, within RR42_RS07620 at 1632.967 kb on + strand, within RR42_RS07620 at 1632.967 kb on + strand, within RR42_RS07620 at 1632.968 kb on - strand, within RR42_RS07620 at 1632.993 kb on + strand, within RR42_RS07620 at 1632.994 kb on - strand, within RR42_RS07620 at 1633.051 kb on + strand, within RR42_RS07620 at 1633.051 kb on + strand, within RR42_RS07620 at 1633.052 kb on - strand, within RR42_RS07620 at 1633.157 kb on - strand at 1633.157 kb on - strand at 1633.379 kb on - strand at 1633.520 kb on + strand at 1633.520 kb on + strand at 1633.563 kb on + strand at 1633.563 kb on + strand at 1633.564 kb on - strand at 1633.564 kb on - strand at 1633.599 kb on + strand at 1633.599 kb on + strand at 1633.599 kb on + strand at 1633.659 kb on + strand, within RR42_RS07625 at 1633.697 kb on + strand, within RR42_RS07625 at 1633.778 kb on + strand, within RR42_RS07625 at 1633.779 kb on - strand, within RR42_RS07625 at 1634.137 kb on + strand, within RR42_RS07625 at 1634.138 kb on - strand, within RR42_RS07625
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.01 mg/ml remove 1,631,926 + RR42_RS07615 0.62 -0.7 1,631,926 + RR42_RS07615 0.62 +0.4 1,631,927 - RR42_RS07615 0.62 -0.3 1,632,324 + +0.0 1,632,401 + -0.1 1,632,401 + -0.1 1,632,442 + +0.3 1,632,492 + -0.5 1,632,492 + -0.6 1,632,492 + -0.3 1,632,493 - -1.3 1,632,541 + -0.7 1,632,541 + -0.0 1,632,667 + RR42_RS07620 0.11 -1.2 1,632,688 + RR42_RS07620 0.15 -1.2 1,632,689 - RR42_RS07620 0.15 +0.0 1,632,866 - RR42_RS07620 0.47 -1.4 1,632,898 + RR42_RS07620 0.53 -0.8 1,632,898 + RR42_RS07620 0.53 -1.0 1,632,967 + RR42_RS07620 0.65 -1.1 1,632,967 + RR42_RS07620 0.65 +0.5 1,632,968 - RR42_RS07620 0.65 +0.4 1,632,993 + RR42_RS07620 0.70 +0.0 1,632,994 - RR42_RS07620 0.70 +0.1 1,633,051 + RR42_RS07620 0.80 -0.3 1,633,051 + RR42_RS07620 0.80 -0.6 1,633,052 - RR42_RS07620 0.81 -1.7 1,633,157 - -0.2 1,633,157 - +0.8 1,633,379 - +0.3 1,633,520 + -0.6 1,633,520 + -0.1 1,633,563 + +0.6 1,633,563 + -0.1 1,633,564 - +0.4 1,633,564 - +0.4 1,633,599 + -0.3 1,633,599 + +0.3 1,633,599 + +0.4 1,633,659 + RR42_RS07625 0.14 +0.7 1,633,697 + RR42_RS07625 0.18 +0.1 1,633,778 + RR42_RS07625 0.26 -1.3 1,633,779 - RR42_RS07625 0.26 -0.4 1,634,137 + RR42_RS07625 0.63 +0.1 1,634,138 - RR42_RS07625 0.63 -0.8
Or see this region's nucleotide sequence