Experiment: R2A with D-Cycloserine 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS06315 and RR42_RS06320 are separated by 25 nucleotides RR42_RS06320 and RR42_RS06325 are separated by 278 nucleotides RR42_RS06325 and RR42_RS06330 are separated by 43 nucleotides RR42_RS06330 and RR42_RS06335 are separated by 83 nucleotides
RR42_RS06315: RR42_RS06315 - RNA methyltransferase, at 1,386,644 to 1,387,645
_RS06315
RR42_RS06320: RR42_RS06320 - 3-methylitaconate isomerase, at 1,387,671 to 1,388,177
_RS06320
RR42_RS06325: RR42_RS06325 - serine O-acetyltransferase, at 1,388,456 to 1,389,199
_RS06325
RR42_RS06330: RR42_RS06330 - UDP-2,3-diacylglucosamine hydrolase, at 1,389,243 to 1,390,031
_RS06330
RR42_RS06335: RR42_RS06335 - cyclophilin, at 1,390,115 to 1,390,615
_RS06335
Position (kb)
1388
1389
1390 Strain fitness (log2 ratio)
-1
0
1 at 1387.564 kb on + strand at 1387.565 kb on - strand at 1387.666 kb on - strand at 1387.666 kb on - strand at 1387.668 kb on + strand at 1387.668 kb on + strand at 1387.668 kb on + strand at 1387.669 kb on - strand at 1387.669 kb on - strand at 1387.669 kb on - strand at 1387.669 kb on - strand at 1387.669 kb on - strand at 1387.669 kb on - strand at 1387.669 kb on - strand at 1388.140 kb on - strand at 1388.140 kb on - strand at 1388.351 kb on + strand at 1388.352 kb on - strand at 1389.014 kb on + strand, within RR42_RS06325 at 1389.014 kb on + strand, within RR42_RS06325 at 1389.197 kb on + strand at 1389.197 kb on + strand at 1389.197 kb on + strand at 1389.197 kb on + strand at 1389.197 kb on + strand at 1389.198 kb on - strand at 1389.250 kb on - strand at 1390.117 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.01 mg/ml remove 1,387,564 + +0.8 1,387,565 - +0.3 1,387,666 - -0.3 1,387,666 - -0.3 1,387,668 + -0.2 1,387,668 + +0.3 1,387,668 + -1.5 1,387,669 - +1.1 1,387,669 - -1.1 1,387,669 - +0.5 1,387,669 - +0.4 1,387,669 - -0.4 1,387,669 - -0.3 1,387,669 - -0.1 1,388,140 - -0.1 1,388,140 - +0.1 1,388,351 + +0.8 1,388,352 - +0.6 1,389,014 + RR42_RS06325 0.75 -0.6 1,389,014 + RR42_RS06325 0.75 -0.8 1,389,197 + -0.4 1,389,197 + +0.9 1,389,197 + +0.2 1,389,197 + +0.1 1,389,197 + -1.2 1,389,198 - +1.4 1,389,250 - +0.5 1,390,117 - +0.2
Or see this region's nucleotide sequence