Experiment: R2A with D-Cycloserine 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS04465 and RR42_RS04470 are separated by 138 nucleotides RR42_RS04470 and RR42_RS04475 are separated by 93 nucleotides
RR42_RS04465: RR42_RS04465 - aspartate aminotransferase, at 998,245 to 999,360
_RS04465
RR42_RS04470: RR42_RS04470 - prephenate dehydrogenase, at 999,499 to 1,000,407
_RS04470
RR42_RS04475: RR42_RS04475 - 3-phosphoshikimate 1-carboxyvinyltransferase, at 1,000,501 to 1,001,811
_RS04475
Position (kb) 999
1000
1001 Strain fitness (log2 ratio) -2
-1
0
1
2 at 998.597 kb on - strand, within RR42_RS04465 at 998.620 kb on + strand, within RR42_RS04465 at 998.737 kb on - strand, within RR42_RS04465 at 998.748 kb on + strand, within RR42_RS04465 at 998.748 kb on + strand, within RR42_RS04465 at 998.749 kb on - strand, within RR42_RS04465 at 998.749 kb on - strand, within RR42_RS04465 at 998.842 kb on + strand, within RR42_RS04465 at 998.842 kb on + strand, within RR42_RS04465 at 998.842 kb on + strand, within RR42_RS04465 at 998.843 kb on - strand, within RR42_RS04465 at 998.843 kb on - strand, within RR42_RS04465 at 998.843 kb on - strand, within RR42_RS04465 at 998.851 kb on + strand, within RR42_RS04465 at 998.851 kb on + strand, within RR42_RS04465 at 998.852 kb on - strand, within RR42_RS04465 at 998.852 kb on - strand, within RR42_RS04465 at 998.852 kb on - strand, within RR42_RS04465 at 998.852 kb on - strand, within RR42_RS04465 at 998.873 kb on - strand, within RR42_RS04465 at 998.899 kb on + strand, within RR42_RS04465 at 998.900 kb on - strand, within RR42_RS04465 at 998.915 kb on + strand, within RR42_RS04465 at 998.938 kb on + strand, within RR42_RS04465 at 998.939 kb on - strand, within RR42_RS04465 at 998.966 kb on - strand, within RR42_RS04465 at 999.154 kb on + strand, within RR42_RS04465 at 999.154 kb on + strand, within RR42_RS04465 at 999.154 kb on + strand, within RR42_RS04465 at 999.154 kb on + strand, within RR42_RS04465 at 999.154 kb on + strand, within RR42_RS04465 at 999.155 kb on - strand, within RR42_RS04465 at 999.155 kb on - strand, within RR42_RS04465 at 999.155 kb on - strand, within RR42_RS04465 at 999.155 kb on - strand, within RR42_RS04465 at 999.155 kb on - strand, within RR42_RS04465 at 999.155 kb on - strand, within RR42_RS04465 at 999.155 kb on - strand, within RR42_RS04465 at 999.254 kb on + strand at 999.295 kb on - strand at 999.358 kb on + strand at 999.359 kb on - strand at 999.454 kb on + strand at 999.454 kb on + strand at 999.454 kb on + strand at 999.454 kb on + strand at 999.454 kb on + strand at 999.455 kb on - strand at 999.455 kb on - strand at 999.455 kb on - strand at 999.492 kb on + strand at 999.787 kb on - strand at 999.934 kb on + strand, within RR42_RS04470 at 999.934 kb on + strand, within RR42_RS04470 at 999.934 kb on + strand, within RR42_RS04470 at 999.934 kb on + strand, within RR42_RS04470 at 999.935 kb on - strand, within RR42_RS04470 at 999.935 kb on - strand, within RR42_RS04470 at 999.935 kb on - strand, within RR42_RS04470 at 1000.267 kb on + strand, within RR42_RS04470 at 1000.267 kb on + strand, within RR42_RS04470 at 1000.268 kb on - strand, within RR42_RS04470 at 1000.332 kb on + strand at 1000.332 kb on + strand at 1000.333 kb on - strand at 1000.488 kb on + strand at 1000.489 kb on - strand at 1000.489 kb on - strand at 1000.828 kb on + strand, within RR42_RS04475 at 1000.829 kb on - strand, within RR42_RS04475 at 1000.851 kb on + strand, within RR42_RS04475 at 1000.852 kb on - strand, within RR42_RS04475 at 1000.852 kb on - strand, within RR42_RS04475 at 1000.852 kb on - strand, within RR42_RS04475 at 1000.852 kb on - strand, within RR42_RS04475 at 1000.898 kb on + strand, within RR42_RS04475 at 1000.898 kb on + strand, within RR42_RS04475 at 1000.915 kb on + strand, within RR42_RS04475 at 1001.104 kb on + strand, within RR42_RS04475 at 1001.248 kb on + strand, within RR42_RS04475 at 1001.249 kb on - strand, within RR42_RS04475 at 1001.347 kb on - strand, within RR42_RS04475
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.01 mg/ml remove 998,597 - RR42_RS04465 0.32 +0.5 998,620 + RR42_RS04465 0.34 +0.2 998,737 - RR42_RS04465 0.44 -2.5 998,748 + RR42_RS04465 0.45 -0.4 998,748 + RR42_RS04465 0.45 -0.5 998,749 - RR42_RS04465 0.45 -0.1 998,749 - RR42_RS04465 0.45 -0.4 998,842 + RR42_RS04465 0.53 +0.2 998,842 + RR42_RS04465 0.53 +1.9 998,842 + RR42_RS04465 0.53 +0.2 998,843 - RR42_RS04465 0.54 +0.2 998,843 - RR42_RS04465 0.54 -0.3 998,843 - RR42_RS04465 0.54 +0.2 998,851 + RR42_RS04465 0.54 -0.3 998,851 + RR42_RS04465 0.54 -0.2 998,852 - RR42_RS04465 0.54 +0.2 998,852 - RR42_RS04465 0.54 -0.5 998,852 - RR42_RS04465 0.54 +0.1 998,852 - RR42_RS04465 0.54 -0.2 998,873 - RR42_RS04465 0.56 -0.8 998,899 + RR42_RS04465 0.59 -0.8 998,900 - RR42_RS04465 0.59 +1.2 998,915 + RR42_RS04465 0.60 -0.5 998,938 + RR42_RS04465 0.62 +0.2 998,939 - RR42_RS04465 0.62 +0.6 998,966 - RR42_RS04465 0.65 +1.2 999,154 + RR42_RS04465 0.81 +0.7 999,154 + RR42_RS04465 0.81 -0.6 999,154 + RR42_RS04465 0.81 -1.2 999,154 + RR42_RS04465 0.81 -0.1 999,154 + RR42_RS04465 0.81 +0.1 999,155 - RR42_RS04465 0.82 +0.7 999,155 - RR42_RS04465 0.82 +0.1 999,155 - RR42_RS04465 0.82 +0.2 999,155 - RR42_RS04465 0.82 +0.1 999,155 - RR42_RS04465 0.82 +0.1 999,155 - RR42_RS04465 0.82 -0.5 999,155 - RR42_RS04465 0.82 +0.0 999,254 + -0.5 999,295 - +0.8 999,358 + -0.1 999,359 - +0.4 999,454 + +0.0 999,454 + +1.5 999,454 + -0.6 999,454 + -0.6 999,454 + +0.3 999,455 - +0.2 999,455 - +0.1 999,455 - -0.9 999,492 + -0.1 999,787 - +0.3 999,934 + RR42_RS04470 0.48 -0.9 999,934 + RR42_RS04470 0.48 -0.6 999,934 + RR42_RS04470 0.48 -0.8 999,934 + RR42_RS04470 0.48 -1.7 999,935 - RR42_RS04470 0.48 -1.9 999,935 - RR42_RS04470 0.48 -0.8 999,935 - RR42_RS04470 0.48 -0.8 1,000,267 + RR42_RS04470 0.84 +0.3 1,000,267 + RR42_RS04470 0.84 +0.8 1,000,268 - RR42_RS04470 0.85 -0.2 1,000,332 + -0.4 1,000,332 + +0.6 1,000,333 - -0.7 1,000,488 + +0.8 1,000,489 - -0.5 1,000,489 - -1.1 1,000,828 + RR42_RS04475 0.25 +0.2 1,000,829 - RR42_RS04475 0.25 -0.6 1,000,851 + RR42_RS04475 0.27 -0.0 1,000,852 - RR42_RS04475 0.27 -2.3 1,000,852 - RR42_RS04475 0.27 -0.8 1,000,852 - RR42_RS04475 0.27 -2.2 1,000,852 - RR42_RS04475 0.27 -0.7 1,000,898 + RR42_RS04475 0.30 -0.7 1,000,898 + RR42_RS04475 0.30 -1.1 1,000,915 + RR42_RS04475 0.32 -0.4 1,001,104 + RR42_RS04475 0.46 -0.7 1,001,248 + RR42_RS04475 0.57 -1.3 1,001,249 - RR42_RS04475 0.57 -0.9 1,001,347 - RR42_RS04475 0.65 -0.3
Or see this region's nucleotide sequence