Experiment: R2A with D-Cycloserine 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS04405 and RR42_RS04410 are separated by 20 nucleotides RR42_RS04410 and RR42_RS04415 are separated by 240 nucleotides
RR42_RS04405: RR42_RS04405 - cyanophycin synthetase, at 979,764 to 982,334
_RS04405
RR42_RS04410: RR42_RS04410 - cyanophycin synthetase, at 982,355 to 984,991
_RS04410
RR42_RS04415: RR42_RS04415 - ABC transporter, at 985,232 to 987,520
_RS04415
Position (kb) 982
983
984
985 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 981.483 kb on - strand, within RR42_RS04405 at 981.485 kb on - strand, within RR42_RS04405 at 981.530 kb on - strand, within RR42_RS04405 at 981.838 kb on + strand, within RR42_RS04405 at 982.024 kb on + strand, within RR42_RS04405 at 982.024 kb on + strand, within RR42_RS04405 at 982.032 kb on + strand, within RR42_RS04405 at 982.032 kb on + strand, within RR42_RS04405 at 982.042 kb on + strand, within RR42_RS04405 at 982.042 kb on + strand, within RR42_RS04405 at 982.042 kb on + strand, within RR42_RS04405 at 982.043 kb on - strand, within RR42_RS04405 at 982.457 kb on + strand at 982.457 kb on + strand at 982.458 kb on - strand at 982.458 kb on - strand at 982.458 kb on - strand at 982.533 kb on + strand at 982.534 kb on - strand at 982.534 kb on - strand at 982.945 kb on - strand, within RR42_RS04410 at 983.025 kb on + strand, within RR42_RS04410 at 983.026 kb on - strand, within RR42_RS04410 at 983.026 kb on - strand, within RR42_RS04410 at 983.205 kb on + strand, within RR42_RS04410 at 983.205 kb on + strand, within RR42_RS04410 at 983.206 kb on - strand, within RR42_RS04410 at 983.206 kb on - strand, within RR42_RS04410 at 983.206 kb on - strand, within RR42_RS04410 at 983.229 kb on + strand, within RR42_RS04410 at 983.229 kb on + strand, within RR42_RS04410 at 983.229 kb on + strand, within RR42_RS04410 at 983.229 kb on + strand, within RR42_RS04410 at 983.229 kb on + strand, within RR42_RS04410 at 983.229 kb on + strand, within RR42_RS04410 at 983.229 kb on + strand, within RR42_RS04410 at 983.230 kb on - strand, within RR42_RS04410 at 983.230 kb on - strand, within RR42_RS04410 at 983.232 kb on + strand, within RR42_RS04410 at 983.292 kb on + strand, within RR42_RS04410 at 983.428 kb on - strand, within RR42_RS04410 at 984.050 kb on + strand, within RR42_RS04410 at 984.050 kb on + strand, within RR42_RS04410 at 984.207 kb on + strand, within RR42_RS04410 at 984.411 kb on + strand, within RR42_RS04410 at 984.411 kb on + strand, within RR42_RS04410 at 984.411 kb on + strand, within RR42_RS04410 at 984.559 kb on - strand, within RR42_RS04410 at 984.654 kb on + strand, within RR42_RS04410 at 984.654 kb on + strand, within RR42_RS04410 at 984.853 kb on - strand at 984.927 kb on + strand at 984.928 kb on - strand at 984.997 kb on + strand at 984.998 kb on - strand at 985.030 kb on - strand at 985.030 kb on - strand at 985.030 kb on - strand at 985.038 kb on - strand at 985.048 kb on + strand at 985.049 kb on - strand at 985.049 kb on - strand at 985.054 kb on + strand at 985.131 kb on + strand at 985.131 kb on + strand at 985.132 kb on - strand at 985.132 kb on - strand at 985.136 kb on - strand at 985.136 kb on - strand at 985.194 kb on - strand at 985.194 kb on - strand at 985.201 kb on + strand at 985.202 kb on - strand at 985.232 kb on - strand at 985.383 kb on + strand at 985.543 kb on + strand, within RR42_RS04415 at 985.544 kb on - strand, within RR42_RS04415 at 985.554 kb on - strand, within RR42_RS04415 at 985.908 kb on - strand, within RR42_RS04415 at 985.985 kb on + strand, within RR42_RS04415 at 985.986 kb on - strand, within RR42_RS04415 at 985.986 kb on - strand, within RR42_RS04415 at 985.986 kb on - strand, within RR42_RS04415
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.01 mg/ml remove 981,483 - RR42_RS04405 0.67 -0.3 981,485 - RR42_RS04405 0.67 -1.7 981,530 - RR42_RS04405 0.69 -1.4 981,838 + RR42_RS04405 0.81 -1.3 982,024 + RR42_RS04405 0.88 -0.3 982,024 + RR42_RS04405 0.88 +0.3 982,032 + RR42_RS04405 0.88 -2.2 982,032 + RR42_RS04405 0.88 -1.0 982,042 + RR42_RS04405 0.89 -1.3 982,042 + RR42_RS04405 0.89 -0.6 982,042 + RR42_RS04405 0.89 -0.0 982,043 - RR42_RS04405 0.89 +3.3 982,457 + +0.1 982,457 + -1.1 982,458 - +0.4 982,458 - +0.1 982,458 - -0.6 982,533 + +1.3 982,534 - +0.9 982,534 - +0.7 982,945 - RR42_RS04410 0.22 -1.6 983,025 + RR42_RS04410 0.25 -1.7 983,026 - RR42_RS04410 0.25 -2.1 983,026 - RR42_RS04410 0.25 -0.7 983,205 + RR42_RS04410 0.32 -0.8 983,205 + RR42_RS04410 0.32 -1.7 983,206 - RR42_RS04410 0.32 +0.8 983,206 - RR42_RS04410 0.32 +0.1 983,206 - RR42_RS04410 0.32 +0.5 983,229 + RR42_RS04410 0.33 -0.4 983,229 + RR42_RS04410 0.33 -0.4 983,229 + RR42_RS04410 0.33 -1.1 983,229 + RR42_RS04410 0.33 -1.4 983,229 + RR42_RS04410 0.33 +0.5 983,229 + RR42_RS04410 0.33 +0.0 983,229 + RR42_RS04410 0.33 +0.0 983,230 - RR42_RS04410 0.33 +0.0 983,230 - RR42_RS04410 0.33 +0.2 983,232 + RR42_RS04410 0.33 -0.2 983,292 + RR42_RS04410 0.36 -2.2 983,428 - RR42_RS04410 0.41 -1.1 984,050 + RR42_RS04410 0.64 -0.5 984,050 + RR42_RS04410 0.64 +1.3 984,207 + RR42_RS04410 0.70 -1.1 984,411 + RR42_RS04410 0.78 -0.1 984,411 + RR42_RS04410 0.78 -0.9 984,411 + RR42_RS04410 0.78 +0.1 984,559 - RR42_RS04410 0.84 -1.1 984,654 + RR42_RS04410 0.87 -0.7 984,654 + RR42_RS04410 0.87 +0.2 984,853 - -1.3 984,927 + -2.4 984,928 - -1.3 984,997 + -1.1 984,998 - +0.0 985,030 - -0.1 985,030 - +0.5 985,030 - -2.3 985,038 - -0.7 985,048 + -3.6 985,049 - -0.1 985,049 - -0.3 985,054 + -1.3 985,131 + +0.7 985,131 + +0.4 985,132 - -1.0 985,132 - -0.5 985,136 - -0.5 985,136 - +0.1 985,194 - -0.5 985,194 - +0.2 985,201 + -0.0 985,202 - -1.2 985,232 - -0.6 985,383 + -0.2 985,543 + RR42_RS04415 0.14 +0.4 985,544 - RR42_RS04415 0.14 -0.1 985,554 - RR42_RS04415 0.14 -1.8 985,908 - RR42_RS04415 0.30 +0.3 985,985 + RR42_RS04415 0.33 -1.4 985,986 - RR42_RS04415 0.33 -1.1 985,986 - RR42_RS04415 0.33 +0.3 985,986 - RR42_RS04415 0.33 -2.3
Or see this region's nucleotide sequence