Experiment: R2A with D-Cycloserine 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS03865 and RR42_RS03870 are separated by 112 nucleotides RR42_RS03870 and RR42_RS03875 are separated by 166 nucleotides RR42_RS03875 and RR42_RS03880 are separated by 47 nucleotides RR42_RS03880 and RR42_RS03885 overlap by 1 nucleotides
RR42_RS03865: RR42_RS03865 - hydroxyacid dehydrogenase, at 854,013 to 854,954
_RS03865
RR42_RS03870: RR42_RS03870 - hypothetical protein, at 855,067 to 855,261
_RS03870
RR42_RS03875: RR42_RS03875 - nucleoside 2-deoxyribosyltransferase, at 855,428 to 855,976
_RS03875
RR42_RS03880: RR42_RS03880 - hypothetical protein, at 856,024 to 856,680
_RS03880
RR42_RS03885: RR42_RS03885 - mammalian cell entry protein, at 856,680 to 858,311
_RS03885
Position (kb) 855
856 Strain fitness (log2 ratio) -1
0
1 at 854.651 kb on - strand, within RR42_RS03865 at 854.836 kb on + strand, within RR42_RS03865 at 854.836 kb on + strand, within RR42_RS03865 at 854.837 kb on - strand, within RR42_RS03865 at 854.990 kb on + strand at 854.993 kb on - strand at 855.080 kb on - strand at 855.080 kb on - strand at 855.081 kb on + strand at 855.440 kb on + strand at 855.440 kb on + strand at 855.440 kb on + strand at 855.515 kb on + strand, within RR42_RS03875 at 855.516 kb on - strand, within RR42_RS03875 at 856.014 kb on + strand at 856.298 kb on + strand, within RR42_RS03880 at 856.667 kb on - strand at 856.947 kb on + strand, within RR42_RS03885
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.01 mg/ml remove 854,651 - RR42_RS03865 0.68 -0.9 854,836 + RR42_RS03865 0.87 +0.5 854,836 + RR42_RS03865 0.87 +0.2 854,837 - RR42_RS03865 0.87 -0.2 854,990 + +1.3 854,993 - -0.4 855,080 - -0.4 855,080 - -0.4 855,081 + -0.1 855,440 + -0.3 855,440 + -1.0 855,440 + -0.4 855,515 + RR42_RS03875 0.16 -0.6 855,516 - RR42_RS03875 0.16 -0.7 856,014 + +0.6 856,298 + RR42_RS03880 0.42 +0.3 856,667 - +0.3 856,947 + RR42_RS03885 0.16 -0.1
Or see this region's nucleotide sequence