Experiment: R2A with D-Cycloserine 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS02550 and RR42_RS02555 are separated by 88 nucleotides RR42_RS02555 and RR42_RS02560 are separated by 60 nucleotides RR42_RS02560 and RR42_RS02565 are separated by 174 nucleotides
RR42_RS02550: RR42_RS02550 - ABC transporter permease, at 574,190 to 574,954
_RS02550
RR42_RS02555: RR42_RS02555 - transcriptional regulator, at 575,043 to 575,501
_RS02555
RR42_RS02560: RR42_RS02560 - cysteine dioxygenase, at 575,562 to 576,158
_RS02560
RR42_RS02565: RR42_RS02565 - LysR family transcriptional regulator, at 576,333 to 577,304
_RS02565
Position (kb) 575
576
577 Strain fitness (log2 ratio) -2
-1
0
1
2 at 574.653 kb on + strand, within RR42_RS02550 at 574.654 kb on - strand, within RR42_RS02550 at 574.654 kb on - strand, within RR42_RS02550 at 574.654 kb on - strand, within RR42_RS02550 at 574.721 kb on + strand, within RR42_RS02550 at 574.721 kb on + strand, within RR42_RS02550 at 574.721 kb on + strand, within RR42_RS02550 at 574.898 kb on + strand at 574.898 kb on + strand at 574.899 kb on - strand at 574.899 kb on - strand at 574.899 kb on - strand at 574.899 kb on - strand at 574.899 kb on - strand at 575.050 kb on + strand at 575.050 kb on + strand at 575.074 kb on + strand at 575.143 kb on + strand, within RR42_RS02555 at 575.156 kb on - strand, within RR42_RS02555 at 575.216 kb on - strand, within RR42_RS02555 at 575.308 kb on - strand, within RR42_RS02555 at 575.362 kb on - strand, within RR42_RS02555 at 575.620 kb on + strand at 575.621 kb on - strand at 575.624 kb on + strand, within RR42_RS02560 at 575.624 kb on + strand, within RR42_RS02560 at 575.624 kb on + strand, within RR42_RS02560 at 575.624 kb on + strand, within RR42_RS02560 at 575.625 kb on - strand, within RR42_RS02560 at 575.625 kb on - strand, within RR42_RS02560 at 575.625 kb on - strand, within RR42_RS02560 at 575.898 kb on + strand, within RR42_RS02560 at 575.898 kb on + strand, within RR42_RS02560 at 575.898 kb on + strand, within RR42_RS02560 at 575.899 kb on - strand, within RR42_RS02560 at 575.899 kb on - strand, within RR42_RS02560 at 575.899 kb on - strand, within RR42_RS02560 at 575.914 kb on + strand, within RR42_RS02560 at 575.914 kb on + strand, within RR42_RS02560 at 575.915 kb on - strand, within RR42_RS02560 at 576.189 kb on + strand at 576.190 kb on - strand at 576.246 kb on + strand at 576.412 kb on + strand at 576.412 kb on + strand at 576.413 kb on - strand at 576.413 kb on - strand at 576.526 kb on - strand, within RR42_RS02565 at 576.526 kb on - strand, within RR42_RS02565 at 576.772 kb on - strand, within RR42_RS02565 at 576.772 kb on - strand, within RR42_RS02565 at 576.792 kb on - strand, within RR42_RS02565 at 576.813 kb on + strand, within RR42_RS02565 at 576.852 kb on + strand, within RR42_RS02565 at 576.852 kb on + strand, within RR42_RS02565 at 576.886 kb on - strand, within RR42_RS02565 at 576.930 kb on + strand, within RR42_RS02565 at 576.930 kb on + strand, within RR42_RS02565 at 576.930 kb on + strand, within RR42_RS02565 at 576.931 kb on - strand, within RR42_RS02565 at 576.931 kb on - strand, within RR42_RS02565 at 576.932 kb on + strand, within RR42_RS02565 at 576.932 kb on + strand, within RR42_RS02565 at 576.933 kb on - strand, within RR42_RS02565 at 577.012 kb on - strand, within RR42_RS02565
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.01 mg/ml remove 574,653 + RR42_RS02550 0.61 +0.7 574,654 - RR42_RS02550 0.61 +1.1 574,654 - RR42_RS02550 0.61 +0.7 574,654 - RR42_RS02550 0.61 -0.0 574,721 + RR42_RS02550 0.69 +0.4 574,721 + RR42_RS02550 0.69 -0.1 574,721 + RR42_RS02550 0.69 +0.1 574,898 + +0.0 574,898 + +0.1 574,899 - -0.7 574,899 - -0.5 574,899 - -0.3 574,899 - +0.4 574,899 - -0.1 575,050 + +0.0 575,050 + -0.3 575,074 + -0.6 575,143 + RR42_RS02555 0.22 -1.9 575,156 - RR42_RS02555 0.25 +0.4 575,216 - RR42_RS02555 0.38 +0.1 575,308 - RR42_RS02555 0.58 +1.3 575,362 - RR42_RS02555 0.69 +0.7 575,620 + +0.4 575,621 - +0.3 575,624 + RR42_RS02560 0.10 +0.9 575,624 + RR42_RS02560 0.10 -0.2 575,624 + RR42_RS02560 0.10 +0.2 575,624 + RR42_RS02560 0.10 +0.2 575,625 - RR42_RS02560 0.11 +0.7 575,625 - RR42_RS02560 0.11 +0.4 575,625 - RR42_RS02560 0.11 +0.0 575,898 + RR42_RS02560 0.56 -0.3 575,898 + RR42_RS02560 0.56 +1.0 575,898 + RR42_RS02560 0.56 +0.7 575,899 - RR42_RS02560 0.56 +1.4 575,899 - RR42_RS02560 0.56 -0.5 575,899 - RR42_RS02560 0.56 -1.8 575,914 + RR42_RS02560 0.59 -0.1 575,914 + RR42_RS02560 0.59 +1.2 575,915 - RR42_RS02560 0.59 +0.7 576,189 + +0.3 576,190 - -0.0 576,246 + +0.2 576,412 + -0.0 576,412 + -1.0 576,413 - +0.4 576,413 - -1.5 576,526 - RR42_RS02565 0.20 +0.4 576,526 - RR42_RS02565 0.20 +0.2 576,772 - RR42_RS02565 0.45 -1.3 576,772 - RR42_RS02565 0.45 +0.2 576,792 - RR42_RS02565 0.47 +1.2 576,813 + RR42_RS02565 0.49 +1.8 576,852 + RR42_RS02565 0.53 -0.0 576,852 + RR42_RS02565 0.53 +0.4 576,886 - RR42_RS02565 0.57 -0.1 576,930 + RR42_RS02565 0.61 +0.6 576,930 + RR42_RS02565 0.61 -1.4 576,930 + RR42_RS02565 0.61 -0.0 576,931 - RR42_RS02565 0.62 +0.8 576,931 - RR42_RS02565 0.62 -0.4 576,932 + RR42_RS02565 0.62 -1.4 576,932 + RR42_RS02565 0.62 +0.2 576,933 - RR42_RS02565 0.62 -0.8 577,012 - RR42_RS02565 0.70 +2.0
Or see this region's nucleotide sequence