Experiment: R2A with D-Cycloserine 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS01900 and RR42_RS01905 are separated by 78 nucleotides RR42_RS01905 and RR42_RS01910 are separated by 109 nucleotides RR42_RS01910 and RR42_RS01915 are separated by 7 nucleotides
RR42_RS01900: RR42_RS01900 - membrane protein, at 427,799 to 428,008
_RS01900
RR42_RS01905: RR42_RS01905 - cytochrome C oxidase subunit II, at 428,087 to 428,848
_RS01905
RR42_RS01910: RR42_RS01910 - cytochrome C oxidase subunit I, at 428,958 to 429,614
_RS01910
RR42_RS01915: RR42_RS01915 - cytochrome C oxidase subunit I, at 429,622 to 430,725
_RS01915
Position (kb) 428
429
430 Strain fitness (log2 ratio) -2
-1
0
1 at 427.966 kb on + strand, within RR42_RS01900 at 427.966 kb on + strand, within RR42_RS01900 at 428.036 kb on - strand at 428.041 kb on + strand at 428.041 kb on + strand at 428.042 kb on - strand at 428.042 kb on - strand at 428.338 kb on + strand, within RR42_RS01905 at 428.339 kb on - strand, within RR42_RS01905 at 428.498 kb on - strand, within RR42_RS01905 at 428.640 kb on - strand, within RR42_RS01905 at 428.640 kb on - strand, within RR42_RS01905 at 428.753 kb on + strand, within RR42_RS01905 at 428.754 kb on - strand, within RR42_RS01905 at 428.760 kb on - strand, within RR42_RS01905 at 428.957 kb on + strand at 428.957 kb on + strand at 428.957 kb on + strand at 428.957 kb on + strand at 428.958 kb on - strand at 428.958 kb on - strand at 428.958 kb on - strand at 428.958 kb on - strand at 429.096 kb on + strand, within RR42_RS01910 at 429.105 kb on + strand, within RR42_RS01910 at 429.105 kb on + strand, within RR42_RS01910 at 429.105 kb on + strand, within RR42_RS01910 at 429.106 kb on - strand, within RR42_RS01910 at 429.108 kb on + strand, within RR42_RS01910 at 429.108 kb on + strand, within RR42_RS01910 at 429.108 kb on + strand, within RR42_RS01910 at 429.109 kb on - strand, within RR42_RS01910 at 429.109 kb on - strand, within RR42_RS01910 at 429.109 kb on - strand, within RR42_RS01910 at 429.141 kb on + strand, within RR42_RS01910 at 429.141 kb on + strand, within RR42_RS01910 at 429.142 kb on - strand, within RR42_RS01910 at 429.142 kb on - strand, within RR42_RS01910 at 429.154 kb on - strand, within RR42_RS01910 at 429.403 kb on - strand, within RR42_RS01910 at 429.597 kb on + strand at 429.598 kb on - strand at 429.676 kb on + strand at 429.677 kb on - strand at 429.677 kb on - strand at 429.677 kb on - strand at 429.677 kb on - strand at 429.677 kb on - strand at 429.677 kb on - strand at 429.818 kb on - strand, within RR42_RS01915 at 429.929 kb on - strand, within RR42_RS01915 at 429.929 kb on - strand, within RR42_RS01915 at 430.273 kb on - strand, within RR42_RS01915 at 430.276 kb on + strand, within RR42_RS01915 at 430.276 kb on + strand, within RR42_RS01915 at 430.393 kb on + strand, within RR42_RS01915 at 430.394 kb on - strand, within RR42_RS01915 at 430.394 kb on - strand, within RR42_RS01915 at 430.394 kb on - strand, within RR42_RS01915
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.01 mg/ml remove 427,966 + RR42_RS01900 0.80 +0.1 427,966 + RR42_RS01900 0.80 -0.0 428,036 - -0.3 428,041 + +0.3 428,041 + +0.0 428,042 - -1.5 428,042 - -0.3 428,338 + RR42_RS01905 0.33 +1.1 428,339 - RR42_RS01905 0.33 -0.6 428,498 - RR42_RS01905 0.54 -0.2 428,640 - RR42_RS01905 0.73 +0.4 428,640 - RR42_RS01905 0.73 +1.1 428,753 + RR42_RS01905 0.87 -1.3 428,754 - RR42_RS01905 0.88 -1.0 428,760 - RR42_RS01905 0.88 +0.5 428,957 + -1.4 428,957 + -0.4 428,957 + -0.8 428,957 + -0.4 428,958 - -0.6 428,958 - -0.2 428,958 - -0.2 428,958 - -0.1 429,096 + RR42_RS01910 0.21 -0.3 429,105 + RR42_RS01910 0.22 -1.3 429,105 + RR42_RS01910 0.22 -0.1 429,105 + RR42_RS01910 0.22 +0.4 429,106 - RR42_RS01910 0.23 +0.1 429,108 + RR42_RS01910 0.23 -0.5 429,108 + RR42_RS01910 0.23 -1.5 429,108 + RR42_RS01910 0.23 -0.6 429,109 - RR42_RS01910 0.23 -0.7 429,109 - RR42_RS01910 0.23 -2.2 429,109 - RR42_RS01910 0.23 -2.0 429,141 + RR42_RS01910 0.28 +0.0 429,141 + RR42_RS01910 0.28 -0.2 429,142 - RR42_RS01910 0.28 -0.0 429,142 - RR42_RS01910 0.28 -1.1 429,154 - RR42_RS01910 0.30 -0.9 429,403 - RR42_RS01910 0.68 -1.4 429,597 + +0.8 429,598 - -0.6 429,676 + +0.4 429,677 - -0.8 429,677 - -0.3 429,677 - -0.9 429,677 - -0.4 429,677 - -0.9 429,677 - -0.7 429,818 - RR42_RS01915 0.18 -1.0 429,929 - RR42_RS01915 0.28 -1.2 429,929 - RR42_RS01915 0.28 -0.6 430,273 - RR42_RS01915 0.59 -0.6 430,276 + RR42_RS01915 0.59 -1.0 430,276 + RR42_RS01915 0.59 +0.2 430,393 + RR42_RS01915 0.70 +0.4 430,394 - RR42_RS01915 0.70 -1.3 430,394 - RR42_RS01915 0.70 -0.6 430,394 - RR42_RS01915 0.70 -0.0
Or see this region's nucleotide sequence