Experiment: R2A with D-Cycloserine 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS01875 and RR42_RS01880 are separated by 23 nucleotides RR42_RS01880 and RR42_RS01885 are separated by 226 nucleotides RR42_RS01885 and RR42_RS01890 are separated by 34 nucleotides
RR42_RS01875: RR42_RS01875 - cytochrome C oxidase subunit II, at 422,649 to 423,911
_RS01875
RR42_RS01880: RR42_RS01880 - cytochrome oxidase subunit I, at 423,935 to 425,539
_RS01880
RR42_RS01885: RR42_RS01885 - cytochrome C oxidase assembly protein, at 425,766 to 426,380
_RS01885
RR42_RS01890: RR42_RS01890 - membrane protein, at 426,415 to 426,627
_RS01890
Position (kb) 423
424
425
426 Strain fitness (log2 ratio) -2
-1
0
1
2 at 423.078 kb on + strand, within RR42_RS01875 at 423.177 kb on + strand, within RR42_RS01875 at 423.177 kb on + strand, within RR42_RS01875 at 423.177 kb on + strand, within RR42_RS01875 at 423.178 kb on - strand, within RR42_RS01875 at 423.432 kb on + strand, within RR42_RS01875 at 423.570 kb on - strand, within RR42_RS01875 at 423.648 kb on - strand, within RR42_RS01875 at 423.723 kb on + strand, within RR42_RS01875 at 423.940 kb on + strand at 423.940 kb on + strand at 423.941 kb on - strand at 423.941 kb on - strand at 423.946 kb on + strand at 424.062 kb on - strand at 424.688 kb on + strand, within RR42_RS01880 at 424.727 kb on + strand, within RR42_RS01880 at 424.727 kb on + strand, within RR42_RS01880 at 424.727 kb on + strand, within RR42_RS01880 at 424.728 kb on - strand, within RR42_RS01880 at 424.805 kb on - strand, within RR42_RS01880 at 424.805 kb on - strand, within RR42_RS01880 at 424.818 kb on - strand, within RR42_RS01880 at 424.913 kb on + strand, within RR42_RS01880 at 424.914 kb on - strand, within RR42_RS01880 at 424.914 kb on - strand, within RR42_RS01880 at 425.105 kb on + strand, within RR42_RS01880 at 425.130 kb on - strand, within RR42_RS01880 at 425.130 kb on - strand, within RR42_RS01880 at 425.174 kb on + strand, within RR42_RS01880 at 425.175 kb on - strand, within RR42_RS01880 at 425.175 kb on - strand, within RR42_RS01880 at 425.175 kb on - strand, within RR42_RS01880 at 425.208 kb on - strand, within RR42_RS01880 at 425.208 kb on - strand, within RR42_RS01880 at 425.394 kb on - strand at 425.394 kb on - strand at 425.537 kb on + strand at 425.537 kb on + strand at 425.537 kb on + strand at 425.563 kb on - strand at 425.759 kb on + strand at 425.886 kb on + strand, within RR42_RS01885 at 425.941 kb on - strand, within RR42_RS01885 at 426.139 kb on - strand, within RR42_RS01885 at 426.282 kb on - strand, within RR42_RS01885 at 426.282 kb on - strand, within RR42_RS01885 at 426.291 kb on + strand, within RR42_RS01885 at 426.292 kb on - strand, within RR42_RS01885 at 426.294 kb on - strand, within RR42_RS01885
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.01 mg/ml remove 423,078 + RR42_RS01875 0.34 -0.6 423,177 + RR42_RS01875 0.42 -0.3 423,177 + RR42_RS01875 0.42 -0.4 423,177 + RR42_RS01875 0.42 -0.6 423,178 - RR42_RS01875 0.42 -0.7 423,432 + RR42_RS01875 0.62 -0.9 423,570 - RR42_RS01875 0.73 -2.6 423,648 - RR42_RS01875 0.79 +1.5 423,723 + RR42_RS01875 0.85 -0.9 423,940 + -1.7 423,940 + -0.9 423,941 - +0.7 423,941 - -0.5 423,946 + +0.3 424,062 - -0.2 424,688 + RR42_RS01880 0.47 -1.7 424,727 + RR42_RS01880 0.49 -0.5 424,727 + RR42_RS01880 0.49 +2.7 424,727 + RR42_RS01880 0.49 +0.8 424,728 - RR42_RS01880 0.49 -0.1 424,805 - RR42_RS01880 0.54 -1.1 424,805 - RR42_RS01880 0.54 +0.9 424,818 - RR42_RS01880 0.55 -0.7 424,913 + RR42_RS01880 0.61 -0.8 424,914 - RR42_RS01880 0.61 -1.1 424,914 - RR42_RS01880 0.61 -0.6 425,105 + RR42_RS01880 0.73 -1.7 425,130 - RR42_RS01880 0.74 -1.1 425,130 - RR42_RS01880 0.74 -1.8 425,174 + RR42_RS01880 0.77 +0.1 425,175 - RR42_RS01880 0.77 -1.2 425,175 - RR42_RS01880 0.77 -0.2 425,175 - RR42_RS01880 0.77 -1.0 425,208 - RR42_RS01880 0.79 -0.3 425,208 - RR42_RS01880 0.79 -2.2 425,394 - -0.1 425,394 - -0.1 425,537 + -0.1 425,537 + +0.5 425,537 + -0.3 425,563 - -0.5 425,759 + -0.5 425,886 + RR42_RS01885 0.20 -1.3 425,941 - RR42_RS01885 0.28 -0.7 426,139 - RR42_RS01885 0.61 -0.9 426,282 - RR42_RS01885 0.84 -1.4 426,282 - RR42_RS01885 0.84 -1.8 426,291 + RR42_RS01885 0.85 -0.5 426,292 - RR42_RS01885 0.86 -0.2 426,294 - RR42_RS01885 0.86 -0.3
Or see this region's nucleotide sequence