Experiment: R2A with D-Cycloserine 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS01865 and RR42_RS01870 are separated by 265 nucleotides RR42_RS01870 and RR42_RS01875 are separated by 57 nucleotides RR42_RS01875 and RR42_RS01880 are separated by 23 nucleotides
RR42_RS01865: RR42_RS01865 - amidophosphoribosyltransferase, at 420,803 to 421,816
_RS01865
RR42_RS01870: RR42_RS01870 - membrane protein, at 422,082 to 422,591
_RS01870
RR42_RS01875: RR42_RS01875 - cytochrome C oxidase subunit II, at 422,649 to 423,911
_RS01875
RR42_RS01880: RR42_RS01880 - cytochrome oxidase subunit I, at 423,935 to 425,539
_RS01880
Position (kb) 422
423
424 Strain fitness (log2 ratio) -2
-1
0
1
2 at 421.814 kb on + strand at 421.814 kb on + strand at 421.920 kb on + strand at 421.921 kb on - strand at 421.952 kb on - strand at 421.952 kb on - strand at 421.953 kb on + strand at 422.059 kb on + strand at 422.059 kb on + strand at 422.060 kb on - strand at 422.060 kb on - strand at 422.217 kb on + strand, within RR42_RS01870 at 422.217 kb on + strand, within RR42_RS01870 at 422.218 kb on - strand, within RR42_RS01870 at 422.359 kb on - strand, within RR42_RS01870 at 422.392 kb on + strand, within RR42_RS01870 at 422.392 kb on + strand, within RR42_RS01870 at 422.538 kb on + strand, within RR42_RS01870 at 422.538 kb on + strand, within RR42_RS01870 at 422.539 kb on - strand, within RR42_RS01870 at 422.539 kb on - strand, within RR42_RS01870 at 422.631 kb on - strand at 422.643 kb on + strand at 422.643 kb on + strand at 422.872 kb on - strand, within RR42_RS01875 at 422.872 kb on - strand, within RR42_RS01875 at 423.078 kb on + strand, within RR42_RS01875 at 423.177 kb on + strand, within RR42_RS01875 at 423.177 kb on + strand, within RR42_RS01875 at 423.177 kb on + strand, within RR42_RS01875 at 423.178 kb on - strand, within RR42_RS01875 at 423.432 kb on + strand, within RR42_RS01875 at 423.570 kb on - strand, within RR42_RS01875 at 423.648 kb on - strand, within RR42_RS01875 at 423.723 kb on + strand, within RR42_RS01875 at 423.940 kb on + strand at 423.940 kb on + strand at 423.941 kb on - strand at 423.941 kb on - strand at 423.946 kb on + strand at 424.062 kb on - strand at 424.688 kb on + strand, within RR42_RS01880 at 424.727 kb on + strand, within RR42_RS01880 at 424.727 kb on + strand, within RR42_RS01880 at 424.727 kb on + strand, within RR42_RS01880 at 424.728 kb on - strand, within RR42_RS01880 at 424.805 kb on - strand, within RR42_RS01880 at 424.805 kb on - strand, within RR42_RS01880 at 424.818 kb on - strand, within RR42_RS01880
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.01 mg/ml remove 421,814 + -1.8 421,814 + +0.7 421,920 + -0.6 421,921 - +0.2 421,952 - -0.7 421,952 - -1.3 421,953 + +1.0 422,059 + -1.1 422,059 + -0.2 422,060 - -1.7 422,060 - -1.0 422,217 + RR42_RS01870 0.26 +0.4 422,217 + RR42_RS01870 0.26 -2.6 422,218 - RR42_RS01870 0.27 -0.7 422,359 - RR42_RS01870 0.54 -0.3 422,392 + RR42_RS01870 0.61 +0.5 422,392 + RR42_RS01870 0.61 -0.1 422,538 + RR42_RS01870 0.89 +0.1 422,538 + RR42_RS01870 0.89 -0.1 422,539 - RR42_RS01870 0.90 +0.9 422,539 - RR42_RS01870 0.90 -1.1 422,631 - -0.3 422,643 + -0.1 422,643 + -0.9 422,872 - RR42_RS01875 0.18 -2.0 422,872 - RR42_RS01875 0.18 -0.6 423,078 + RR42_RS01875 0.34 -0.6 423,177 + RR42_RS01875 0.42 -0.3 423,177 + RR42_RS01875 0.42 -0.4 423,177 + RR42_RS01875 0.42 -0.6 423,178 - RR42_RS01875 0.42 -0.7 423,432 + RR42_RS01875 0.62 -0.9 423,570 - RR42_RS01875 0.73 -2.6 423,648 - RR42_RS01875 0.79 +1.5 423,723 + RR42_RS01875 0.85 -0.9 423,940 + -1.7 423,940 + -0.9 423,941 - +0.7 423,941 - -0.5 423,946 + +0.3 424,062 - -0.2 424,688 + RR42_RS01880 0.47 -1.7 424,727 + RR42_RS01880 0.49 -0.5 424,727 + RR42_RS01880 0.49 +2.7 424,727 + RR42_RS01880 0.49 +0.8 424,728 - RR42_RS01880 0.49 -0.1 424,805 - RR42_RS01880 0.54 -1.1 424,805 - RR42_RS01880 0.54 +0.9 424,818 - RR42_RS01880 0.55 -0.7
Or see this region's nucleotide sequence