Experiment: Chlorite 0.00005 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4537 and Pf6N2E2_4538 overlap by 1 nucleotides Pf6N2E2_4538 and Pf6N2E2_4539 are separated by 15 nucleotides Pf6N2E2_4539 and Pf6N2E2_4540 are separated by 419 nucleotides Pf6N2E2_4540 and Pf6N2E2_4541 are separated by 124 nucleotides
Pf6N2E2_4537 - Type III secretion inner membrane protein (YscS,homologous to flagellar export components), at 5,271,079 to 5,271,333
_4537
Pf6N2E2_4538 - Type III secretion inner membrane protein (YscT,HrcT,SpaR,EscT,EpaR1,homologous to flagellar export components), at 5,271,333 to 5,272,130
_4538
Pf6N2E2_4539 - Type III secretion inner membrane protein (YscU,SpaS,EscU,HrcU,SsaU, homologous to flagellar export components), at 5,272,146 to 5,273,246
_4539
Pf6N2E2_4540 - hypothetical protein, at 5,273,666 to 5,273,881
_4540
Pf6N2E2_4541 - hypothetical protein, at 5,274,006 to 5,274,455
_4541
Position (kb)
5272
5273
5274 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 5271.167 kb on + strand, within Pf6N2E2_4537 at 5271.167 kb on + strand, within Pf6N2E2_4537 at 5271.328 kb on - strand at 5271.357 kb on + strand at 5271.403 kb on - strand at 5271.414 kb on + strand, within Pf6N2E2_4538 at 5271.621 kb on + strand, within Pf6N2E2_4538 at 5271.621 kb on + strand, within Pf6N2E2_4538 at 5271.621 kb on + strand, within Pf6N2E2_4538 at 5271.622 kb on - strand, within Pf6N2E2_4538 at 5271.622 kb on - strand, within Pf6N2E2_4538 at 5271.798 kb on + strand, within Pf6N2E2_4538 at 5271.798 kb on + strand, within Pf6N2E2_4538 at 5271.798 kb on + strand, within Pf6N2E2_4538 at 5271.798 kb on + strand, within Pf6N2E2_4538 at 5271.798 kb on + strand, within Pf6N2E2_4538 at 5271.964 kb on - strand, within Pf6N2E2_4538 at 5271.964 kb on - strand, within Pf6N2E2_4538 at 5271.964 kb on - strand, within Pf6N2E2_4538 at 5271.965 kb on + strand, within Pf6N2E2_4538 at 5272.039 kb on - strand, within Pf6N2E2_4538 at 5272.427 kb on - strand, within Pf6N2E2_4539 at 5272.465 kb on + strand, within Pf6N2E2_4539 at 5272.618 kb on - strand, within Pf6N2E2_4539 at 5272.680 kb on + strand, within Pf6N2E2_4539 at 5272.680 kb on + strand, within Pf6N2E2_4539 at 5272.735 kb on + strand, within Pf6N2E2_4539 at 5272.735 kb on + strand, within Pf6N2E2_4539 at 5272.735 kb on + strand, within Pf6N2E2_4539 at 5272.735 kb on + strand, within Pf6N2E2_4539 at 5272.735 kb on + strand, within Pf6N2E2_4539 at 5272.735 kb on + strand, within Pf6N2E2_4539 at 5272.736 kb on - strand, within Pf6N2E2_4539 at 5272.736 kb on - strand, within Pf6N2E2_4539 at 5272.736 kb on - strand, within Pf6N2E2_4539 at 5272.736 kb on - strand, within Pf6N2E2_4539 at 5272.776 kb on + strand, within Pf6N2E2_4539 at 5272.776 kb on + strand, within Pf6N2E2_4539 at 5272.855 kb on + strand, within Pf6N2E2_4539 at 5272.855 kb on + strand, within Pf6N2E2_4539 at 5272.980 kb on + strand, within Pf6N2E2_4539 at 5272.980 kb on + strand, within Pf6N2E2_4539 at 5272.981 kb on - strand, within Pf6N2E2_4539 at 5273.113 kb on - strand, within Pf6N2E2_4539 at 5273.133 kb on + strand, within Pf6N2E2_4539 at 5273.173 kb on - strand at 5273.266 kb on + strand at 5273.394 kb on + strand at 5273.761 kb on + strand, within Pf6N2E2_4540 at 5273.761 kb on + strand, within Pf6N2E2_4540 at 5273.761 kb on + strand, within Pf6N2E2_4540 at 5273.762 kb on - strand, within Pf6N2E2_4540 at 5273.897 kb on + strand at 5273.897 kb on + strand at 5273.897 kb on + strand at 5273.898 kb on - strand at 5273.999 kb on + strand at 5273.999 kb on + strand at 5274.000 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Chlorite 0.00005 M remove 5,271,167 + Pf6N2E2_4537 0.35 +0.3 5,271,167 + Pf6N2E2_4537 0.35 -0.3 5,271,328 - -1.5 5,271,357 + -0.6 5,271,403 - +0.6 5,271,414 + Pf6N2E2_4538 0.10 -1.7 5,271,621 + Pf6N2E2_4538 0.36 +0.4 5,271,621 + Pf6N2E2_4538 0.36 +0.8 5,271,621 + Pf6N2E2_4538 0.36 -0.7 5,271,622 - Pf6N2E2_4538 0.36 +0.4 5,271,622 - Pf6N2E2_4538 0.36 -0.5 5,271,798 + Pf6N2E2_4538 0.58 +0.7 5,271,798 + Pf6N2E2_4538 0.58 -0.1 5,271,798 + Pf6N2E2_4538 0.58 -1.8 5,271,798 + Pf6N2E2_4538 0.58 +0.7 5,271,798 + Pf6N2E2_4538 0.58 +1.2 5,271,964 - Pf6N2E2_4538 0.79 -1.7 5,271,964 - Pf6N2E2_4538 0.79 +0.5 5,271,964 - Pf6N2E2_4538 0.79 +1.9 5,271,965 + Pf6N2E2_4538 0.79 +0.4 5,272,039 - Pf6N2E2_4538 0.88 -0.3 5,272,427 - Pf6N2E2_4539 0.26 +0.5 5,272,465 + Pf6N2E2_4539 0.29 +0.6 5,272,618 - Pf6N2E2_4539 0.43 +0.2 5,272,680 + Pf6N2E2_4539 0.49 +1.2 5,272,680 + Pf6N2E2_4539 0.49 -0.2 5,272,735 + Pf6N2E2_4539 0.53 +0.0 5,272,735 + Pf6N2E2_4539 0.53 +0.5 5,272,735 + Pf6N2E2_4539 0.53 -0.7 5,272,735 + Pf6N2E2_4539 0.53 +0.9 5,272,735 + Pf6N2E2_4539 0.53 -0.2 5,272,735 + Pf6N2E2_4539 0.53 -0.0 5,272,736 - Pf6N2E2_4539 0.54 +1.9 5,272,736 - Pf6N2E2_4539 0.54 +0.6 5,272,736 - Pf6N2E2_4539 0.54 -0.2 5,272,736 - Pf6N2E2_4539 0.54 +1.1 5,272,776 + Pf6N2E2_4539 0.57 +0.6 5,272,776 + Pf6N2E2_4539 0.57 +0.2 5,272,855 + Pf6N2E2_4539 0.64 +0.9 5,272,855 + Pf6N2E2_4539 0.64 +0.7 5,272,980 + Pf6N2E2_4539 0.76 -1.1 5,272,980 + Pf6N2E2_4539 0.76 +0.1 5,272,981 - Pf6N2E2_4539 0.76 +0.1 5,273,113 - Pf6N2E2_4539 0.88 -0.2 5,273,133 + Pf6N2E2_4539 0.90 +1.2 5,273,173 - -0.4 5,273,266 + +0.1 5,273,394 + -1.1 5,273,761 + Pf6N2E2_4540 0.44 +0.1 5,273,761 + Pf6N2E2_4540 0.44 -0.1 5,273,761 + Pf6N2E2_4540 0.44 +0.9 5,273,762 - Pf6N2E2_4540 0.44 +1.5 5,273,897 + +0.5 5,273,897 + -0.2 5,273,897 + +0.2 5,273,898 - +1.0 5,273,999 + +1.1 5,273,999 + -0.6 5,274,000 - -0.1
Or see this region's nucleotide sequence