Experiment: Cytosine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4327 and Pf1N1B4_4328 are separated by 26 nucleotides Pf1N1B4_4328 and Pf1N1B4_4329 are separated by 267 nucleotides Pf1N1B4_4329 and Pf1N1B4_4330 are separated by 21 nucleotides
Pf1N1B4_4327 - Spermidine/putrescine-binding periplasmic protein, at 4,787,652 to 4,788,785
_4327
Pf1N1B4_4328 - Agmatine deiminase (EC 3.5.3.12), at 4,788,812 to 4,789,936
_4328
Pf1N1B4_4329 - Acyl dehydratase, at 4,790,204 to 4,790,689
_4329
Pf1N1B4_4330 - 3-methyl-2-oxobutanoate hydroxymethyltransferase (EC 2.1.2.11), at 4,790,711 to 4,791,553
_4330
Position (kb)
4788
4789
4790 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 4787.850 kb on + strand, within Pf1N1B4_4327 at 4787.850 kb on + strand, within Pf1N1B4_4327 at 4787.850 kb on + strand, within Pf1N1B4_4327 at 4787.850 kb on + strand, within Pf1N1B4_4327 at 4788.042 kb on + strand, within Pf1N1B4_4327 at 4788.042 kb on + strand, within Pf1N1B4_4327 at 4788.042 kb on + strand, within Pf1N1B4_4327 at 4788.042 kb on + strand, within Pf1N1B4_4327 at 4788.043 kb on - strand at 4788.043 kb on - strand at 4788.072 kb on + strand, within Pf1N1B4_4327 at 4788.073 kb on - strand, within Pf1N1B4_4327 at 4788.099 kb on + strand at 4788.201 kb on - strand, within Pf1N1B4_4327 at 4788.201 kb on - strand, within Pf1N1B4_4327 at 4788.249 kb on + strand, within Pf1N1B4_4327 at 4788.250 kb on - strand, within Pf1N1B4_4327 at 4788.320 kb on - strand, within Pf1N1B4_4327 at 4788.327 kb on + strand, within Pf1N1B4_4327 at 4788.332 kb on - strand, within Pf1N1B4_4327 at 4788.543 kb on + strand, within Pf1N1B4_4327 at 4788.544 kb on - strand, within Pf1N1B4_4327 at 4788.672 kb on + strand at 4788.672 kb on + strand at 4788.673 kb on - strand at 4788.673 kb on - strand at 4788.690 kb on + strand at 4789.061 kb on + strand, within Pf1N1B4_4328 at 4789.139 kb on + strand, within Pf1N1B4_4328 at 4789.140 kb on - strand, within Pf1N1B4_4328 at 4789.268 kb on - strand, within Pf1N1B4_4328 at 4789.351 kb on + strand, within Pf1N1B4_4328 at 4789.755 kb on - strand, within Pf1N1B4_4328 at 4789.810 kb on + strand, within Pf1N1B4_4328 at 4789.811 kb on - strand, within Pf1N1B4_4328 at 4789.860 kb on + strand at 4789.861 kb on - strand at 4790.121 kb on - strand at 4790.210 kb on - strand at 4790.527 kb on + strand, within Pf1N1B4_4329 at 4790.575 kb on - strand, within Pf1N1B4_4329 at 4790.605 kb on + strand, within Pf1N1B4_4329 at 4790.641 kb on + strand at 4790.641 kb on + strand at 4790.641 kb on + strand at 4790.641 kb on + strand at 4790.867 kb on + strand, within Pf1N1B4_4330 at 4790.909 kb on - strand, within Pf1N1B4_4330
Per-strain Table
Position Strand Gene LocusTag Fraction Cytosine (N) remove 4,787,850 + Pf1N1B4_4327 0.17 +1.0 4,787,850 + Pf1N1B4_4327 0.17 +0.3 4,787,850 + Pf1N1B4_4327 0.17 -0.8 4,787,850 + Pf1N1B4_4327 0.17 +1.2 4,788,042 + Pf1N1B4_4327 0.34 +0.0 4,788,042 + Pf1N1B4_4327 0.34 -0.6 4,788,042 + Pf1N1B4_4327 0.34 -0.4 4,788,042 + Pf1N1B4_4327 0.34 -1.4 4,788,043 - -1.4 4,788,043 - -0.4 4,788,072 + Pf1N1B4_4327 0.37 -0.2 4,788,073 - Pf1N1B4_4327 0.37 -0.4 4,788,099 + -1.4 4,788,201 - Pf1N1B4_4327 0.48 -0.4 4,788,201 - Pf1N1B4_4327 0.48 -0.6 4,788,249 + Pf1N1B4_4327 0.53 +0.4 4,788,250 - Pf1N1B4_4327 0.53 -0.9 4,788,320 - Pf1N1B4_4327 0.59 -1.0 4,788,327 + Pf1N1B4_4327 0.60 +0.4 4,788,332 - Pf1N1B4_4327 0.60 -0.0 4,788,543 + Pf1N1B4_4327 0.79 +1.0 4,788,544 - Pf1N1B4_4327 0.79 +0.6 4,788,672 + -0.5 4,788,672 + -1.4 4,788,673 - +1.4 4,788,673 - -0.4 4,788,690 + -2.9 4,789,061 + Pf1N1B4_4328 0.22 +0.6 4,789,139 + Pf1N1B4_4328 0.29 +0.1 4,789,140 - Pf1N1B4_4328 0.29 -0.4 4,789,268 - Pf1N1B4_4328 0.41 +0.2 4,789,351 + Pf1N1B4_4328 0.48 +0.2 4,789,755 - Pf1N1B4_4328 0.84 +1.1 4,789,810 + Pf1N1B4_4328 0.89 +0.4 4,789,811 - Pf1N1B4_4328 0.89 +1.4 4,789,860 + +0.1 4,789,861 - +0.2 4,790,121 - +0.6 4,790,210 - -1.4 4,790,527 + Pf1N1B4_4329 0.66 -1.4 4,790,575 - Pf1N1B4_4329 0.76 +2.1 4,790,605 + Pf1N1B4_4329 0.83 +0.6 4,790,641 + +0.6 4,790,641 + -1.4 4,790,641 + -0.0 4,790,641 + +0.4 4,790,867 + Pf1N1B4_4330 0.19 +0.2 4,790,909 - Pf1N1B4_4330 0.23 +0.2
Or see this region's nucleotide sequence