Experiment: Cytosine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2537 and Pf1N1B4_2538 are separated by 327 nucleotides Pf1N1B4_2538 and Pf1N1B4_2539 are separated by 49 nucleotides
Pf1N1B4_2537 - DNA-binding response regulator, LuxR family, near polyamine transporter, at 2,878,872 to 2,879,513
_2537
Pf1N1B4_2538 - Putrescine transport ATP-binding protein PotA (TC 3.A.1.11.1), at 2,879,841 to 2,880,965
_2538
Pf1N1B4_2539 - ABC transporter, periplasmic spermidine putrescine-binding protein PotD (TC 3.A.1.11.1), at 2,881,015 to 2,882,058
_2539
Position (kb)
2879
2880
2881 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2878.847 kb on - strand at 2878.847 kb on - strand at 2878.864 kb on + strand at 2878.864 kb on + strand at 2878.864 kb on + strand at 2878.864 kb on + strand at 2878.864 kb on + strand at 2878.864 kb on + strand at 2878.864 kb on + strand at 2878.865 kb on - strand at 2878.865 kb on - strand at 2878.865 kb on - strand at 2878.865 kb on - strand at 2878.865 kb on - strand at 2878.865 kb on - strand at 2878.894 kb on + strand at 2878.894 kb on + strand at 2878.894 kb on + strand at 2878.895 kb on - strand at 2878.895 kb on - strand at 2879.046 kb on - strand, within Pf1N1B4_2537 at 2879.046 kb on - strand, within Pf1N1B4_2537 at 2879.185 kb on - strand, within Pf1N1B4_2537 at 2879.253 kb on + strand, within Pf1N1B4_2537 at 2879.254 kb on - strand, within Pf1N1B4_2537 at 2879.254 kb on - strand, within Pf1N1B4_2537 at 2879.487 kb on + strand at 2879.487 kb on + strand at 2879.488 kb on - strand at 2879.488 kb on - strand at 2879.488 kb on - strand at 2879.653 kb on - strand at 2879.717 kb on - strand at 2879.717 kb on - strand at 2879.799 kb on + strand at 2879.800 kb on - strand at 2879.805 kb on + strand at 2879.806 kb on - strand at 2879.911 kb on - strand at 2879.911 kb on - strand at 2880.126 kb on + strand, within Pf1N1B4_2538 at 2880.127 kb on - strand, within Pf1N1B4_2538 at 2880.127 kb on - strand, within Pf1N1B4_2538 at 2880.127 kb on - strand, within Pf1N1B4_2538 at 2880.127 kb on - strand, within Pf1N1B4_2538 at 2880.127 kb on - strand, within Pf1N1B4_2538 at 2880.441 kb on + strand, within Pf1N1B4_2538 at 2880.441 kb on + strand, within Pf1N1B4_2538 at 2880.441 kb on + strand, within Pf1N1B4_2538 at 2880.442 kb on - strand at 2880.540 kb on + strand, within Pf1N1B4_2538 at 2880.540 kb on + strand, within Pf1N1B4_2538 at 2880.541 kb on - strand, within Pf1N1B4_2538 at 2880.795 kb on + strand, within Pf1N1B4_2538 at 2880.795 kb on + strand, within Pf1N1B4_2538 at 2880.795 kb on + strand, within Pf1N1B4_2538 at 2880.795 kb on + strand, within Pf1N1B4_2538 at 2880.795 kb on + strand, within Pf1N1B4_2538 at 2880.795 kb on + strand, within Pf1N1B4_2538 at 2880.795 kb on + strand, within Pf1N1B4_2538 at 2880.796 kb on - strand at 2880.952 kb on + strand at 2881.012 kb on + strand at 2881.012 kb on + strand at 2881.012 kb on + strand at 2881.012 kb on + strand at 2881.012 kb on + strand at 2881.012 kb on + strand at 2881.012 kb on + strand at 2881.012 kb on + strand at 2881.012 kb on + strand at 2881.013 kb on - strand at 2881.013 kb on - strand at 2881.013 kb on - strand at 2881.013 kb on - strand at 2881.013 kb on - strand at 2881.013 kb on - strand at 2881.013 kb on - strand at 2881.147 kb on + strand, within Pf1N1B4_2539 at 2881.148 kb on - strand, within Pf1N1B4_2539 at 2881.148 kb on - strand, within Pf1N1B4_2539 at 2881.195 kb on + strand, within Pf1N1B4_2539 at 2881.195 kb on + strand, within Pf1N1B4_2539 at 2881.195 kb on + strand, within Pf1N1B4_2539 at 2881.259 kb on + strand, within Pf1N1B4_2539 at 2881.260 kb on - strand at 2881.348 kb on + strand, within Pf1N1B4_2539 at 2881.349 kb on - strand, within Pf1N1B4_2539 at 2881.510 kb on + strand, within Pf1N1B4_2539 at 2881.510 kb on + strand, within Pf1N1B4_2539 at 2881.510 kb on + strand, within Pf1N1B4_2539 at 2881.511 kb on - strand, within Pf1N1B4_2539 at 2881.561 kb on + strand, within Pf1N1B4_2539 at 2881.561 kb on + strand, within Pf1N1B4_2539 at 2881.568 kb on + strand, within Pf1N1B4_2539 at 2881.568 kb on + strand, within Pf1N1B4_2539 at 2881.568 kb on + strand, within Pf1N1B4_2539 at 2881.666 kb on + strand, within Pf1N1B4_2539 at 2881.702 kb on + strand, within Pf1N1B4_2539 at 2881.768 kb on + strand, within Pf1N1B4_2539 at 2881.769 kb on - strand, within Pf1N1B4_2539 at 2881.873 kb on + strand, within Pf1N1B4_2539 at 2881.874 kb on - strand, within Pf1N1B4_2539
Per-strain Table
Position Strand Gene LocusTag Fraction Cytosine (N) remove 2,878,847 - -0.1 2,878,847 - +1.8 2,878,864 + -0.8 2,878,864 + +1.2 2,878,864 + +0.2 2,878,864 + +1.2 2,878,864 + +0.6 2,878,864 + -0.0 2,878,864 + +0.2 2,878,865 - -1.8 2,878,865 - +0.4 2,878,865 - +1.2 2,878,865 - -0.1 2,878,865 - +1.2 2,878,865 - +1.2 2,878,894 + -1.2 2,878,894 + +2.0 2,878,894 + +0.8 2,878,895 - -0.0 2,878,895 - +0.4 2,879,046 - Pf1N1B4_2537 0.27 +1.2 2,879,046 - Pf1N1B4_2537 0.27 +1.8 2,879,185 - Pf1N1B4_2537 0.49 -0.2 2,879,253 + Pf1N1B4_2537 0.59 +1.1 2,879,254 - Pf1N1B4_2537 0.60 +0.3 2,879,254 - Pf1N1B4_2537 0.60 -0.1 2,879,487 + +0.5 2,879,487 + -0.8 2,879,488 - -0.2 2,879,488 - -0.2 2,879,488 - -0.2 2,879,653 - +0.3 2,879,717 - -1.6 2,879,717 - -0.0 2,879,799 + -0.2 2,879,800 - +0.0 2,879,805 + +0.8 2,879,806 - +1.5 2,879,911 - -1.8 2,879,911 - +1.2 2,880,126 + Pf1N1B4_2538 0.25 -1.2 2,880,127 - Pf1N1B4_2538 0.25 -1.8 2,880,127 - Pf1N1B4_2538 0.25 -0.1 2,880,127 - Pf1N1B4_2538 0.25 -0.4 2,880,127 - Pf1N1B4_2538 0.25 +0.8 2,880,127 - Pf1N1B4_2538 0.25 -1.4 2,880,441 + Pf1N1B4_2538 0.53 -1.4 2,880,441 + Pf1N1B4_2538 0.53 +0.3 2,880,441 + Pf1N1B4_2538 0.53 +1.2 2,880,442 - -0.8 2,880,540 + Pf1N1B4_2538 0.62 +1.5 2,880,540 + Pf1N1B4_2538 0.62 -0.4 2,880,541 - Pf1N1B4_2538 0.62 -0.4 2,880,795 + Pf1N1B4_2538 0.85 -0.8 2,880,795 + Pf1N1B4_2538 0.85 -0.4 2,880,795 + Pf1N1B4_2538 0.85 -1.6 2,880,795 + Pf1N1B4_2538 0.85 -0.1 2,880,795 + Pf1N1B4_2538 0.85 +1.8 2,880,795 + Pf1N1B4_2538 0.85 +0.3 2,880,795 + Pf1N1B4_2538 0.85 -0.4 2,880,796 - -0.8 2,880,952 + +0.7 2,881,012 + +1.2 2,881,012 + +0.4 2,881,012 + -0.5 2,881,012 + +2.5 2,881,012 + +0.8 2,881,012 + +0.6 2,881,012 + -0.1 2,881,012 + -0.4 2,881,012 + -0.4 2,881,013 - -1.2 2,881,013 - +0.8 2,881,013 - +0.6 2,881,013 - +0.5 2,881,013 - -0.3 2,881,013 - -1.8 2,881,013 - -0.8 2,881,147 + Pf1N1B4_2539 0.13 +0.2 2,881,148 - Pf1N1B4_2539 0.13 +0.3 2,881,148 - Pf1N1B4_2539 0.13 +0.2 2,881,195 + Pf1N1B4_2539 0.17 +1.3 2,881,195 + Pf1N1B4_2539 0.17 -1.2 2,881,195 + Pf1N1B4_2539 0.17 +1.8 2,881,259 + Pf1N1B4_2539 0.23 -0.8 2,881,260 - +1.5 2,881,348 + Pf1N1B4_2539 0.32 +0.2 2,881,349 - Pf1N1B4_2539 0.32 +1.8 2,881,510 + Pf1N1B4_2539 0.47 +1.2 2,881,510 + Pf1N1B4_2539 0.47 -0.3 2,881,510 + Pf1N1B4_2539 0.47 -0.2 2,881,511 - Pf1N1B4_2539 0.48 -0.2 2,881,561 + Pf1N1B4_2539 0.52 +2.2 2,881,561 + Pf1N1B4_2539 0.52 +0.2 2,881,568 + Pf1N1B4_2539 0.53 +0.2 2,881,568 + Pf1N1B4_2539 0.53 -0.2 2,881,568 + Pf1N1B4_2539 0.53 +0.8 2,881,666 + Pf1N1B4_2539 0.62 +1.2 2,881,702 + Pf1N1B4_2539 0.66 -0.8 2,881,768 + Pf1N1B4_2539 0.72 +0.8 2,881,769 - Pf1N1B4_2539 0.72 +0.8 2,881,873 + Pf1N1B4_2539 0.82 -0.8 2,881,874 - Pf1N1B4_2539 0.82 +1.6
Or see this region's nucleotide sequence