Experiment: Cytosine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1899 and Pf1N1B4_1900 are separated by 0 nucleotides Pf1N1B4_1900 and Pf1N1B4_1901 are separated by 84 nucleotides Pf1N1B4_1901 and Pf1N1B4_1902 are separated by 69 nucleotides
Pf1N1B4_1899 - Gfa-like protein, at 2,173,730 to 2,174,131
_1899
Pf1N1B4_1900 - Aromatic ring-cleaving dioxygenase, at 2,174,132 to 2,174,458
_1900
Pf1N1B4_1901 - Putative NADH-flavin reductase, at 2,174,543 to 2,175,205
_1901
Pf1N1B4_1902 - Transcriptional regulator, LysR family, at 2,175,275 to 2,176,225
_1902
Position (kb)
2174
2175
2176 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2173.549 kb on + strand at 2173.550 kb on - strand at 2173.568 kb on + strand at 2173.568 kb on + strand at 2173.569 kb on - strand at 2173.620 kb on - strand at 2173.696 kb on - strand at 2173.696 kb on - strand at 2173.696 kb on - strand at 2173.705 kb on - strand at 2173.881 kb on + strand, within Pf1N1B4_1899 at 2173.882 kb on - strand, within Pf1N1B4_1899 at 2173.882 kb on - strand, within Pf1N1B4_1899 at 2173.914 kb on - strand, within Pf1N1B4_1899 at 2173.941 kb on - strand, within Pf1N1B4_1899 at 2174.056 kb on + strand, within Pf1N1B4_1899 at 2174.056 kb on + strand, within Pf1N1B4_1899 at 2174.056 kb on + strand, within Pf1N1B4_1899 at 2174.056 kb on + strand, within Pf1N1B4_1899 at 2174.057 kb on - strand, within Pf1N1B4_1899 at 2174.057 kb on - strand, within Pf1N1B4_1899 at 2174.057 kb on - strand, within Pf1N1B4_1899 at 2174.057 kb on - strand, within Pf1N1B4_1899 at 2174.228 kb on + strand, within Pf1N1B4_1900 at 2174.228 kb on + strand, within Pf1N1B4_1900 at 2174.228 kb on + strand, within Pf1N1B4_1900 at 2174.229 kb on - strand, within Pf1N1B4_1900 at 2174.229 kb on - strand, within Pf1N1B4_1900 at 2174.267 kb on + strand, within Pf1N1B4_1900 at 2174.267 kb on + strand, within Pf1N1B4_1900 at 2174.267 kb on + strand, within Pf1N1B4_1900 at 2174.268 kb on - strand, within Pf1N1B4_1900 at 2174.268 kb on - strand, within Pf1N1B4_1900 at 2174.268 kb on - strand, within Pf1N1B4_1900 at 2174.280 kb on + strand, within Pf1N1B4_1900 at 2174.280 kb on + strand, within Pf1N1B4_1900 at 2174.280 kb on + strand, within Pf1N1B4_1900 at 2174.280 kb on + strand, within Pf1N1B4_1900 at 2174.280 kb on + strand, within Pf1N1B4_1900 at 2174.280 kb on + strand, within Pf1N1B4_1900 at 2174.280 kb on + strand, within Pf1N1B4_1900 at 2174.280 kb on + strand, within Pf1N1B4_1900 at 2174.280 kb on + strand, within Pf1N1B4_1900 at 2174.280 kb on + strand, within Pf1N1B4_1900 at 2174.280 kb on + strand, within Pf1N1B4_1900 at 2174.280 kb on + strand, within Pf1N1B4_1900 at 2174.280 kb on + strand, within Pf1N1B4_1900 at 2174.281 kb on - strand, within Pf1N1B4_1900 at 2174.281 kb on - strand, within Pf1N1B4_1900 at 2174.281 kb on - strand, within Pf1N1B4_1900 at 2174.281 kb on - strand, within Pf1N1B4_1900 at 2174.281 kb on - strand, within Pf1N1B4_1900 at 2174.281 kb on - strand, within Pf1N1B4_1900 at 2174.281 kb on - strand, within Pf1N1B4_1900 at 2174.281 kb on - strand, within Pf1N1B4_1900 at 2174.281 kb on - strand, within Pf1N1B4_1900 at 2174.281 kb on - strand, within Pf1N1B4_1900 at 2174.281 kb on - strand, within Pf1N1B4_1900 at 2174.281 kb on - strand, within Pf1N1B4_1900 at 2174.281 kb on - strand, within Pf1N1B4_1900 at 2174.424 kb on + strand, within Pf1N1B4_1900 at 2174.424 kb on + strand, within Pf1N1B4_1900 at 2174.424 kb on + strand, within Pf1N1B4_1900 at 2174.424 kb on + strand, within Pf1N1B4_1900 at 2174.425 kb on - strand, within Pf1N1B4_1900 at 2174.425 kb on - strand, within Pf1N1B4_1900 at 2174.425 kb on - strand, within Pf1N1B4_1900 at 2174.439 kb on + strand at 2174.470 kb on + strand at 2174.475 kb on + strand at 2174.475 kb on + strand at 2174.475 kb on + strand at 2174.475 kb on + strand at 2174.475 kb on + strand at 2174.476 kb on - strand at 2174.476 kb on - strand at 2174.476 kb on - strand at 2174.476 kb on - strand at 2174.476 kb on - strand at 2174.476 kb on - strand at 2174.476 kb on - strand at 2174.514 kb on + strand at 2174.514 kb on + strand at 2174.514 kb on + strand at 2174.514 kb on + strand at 2174.514 kb on + strand at 2174.514 kb on + strand at 2174.514 kb on + strand at 2174.514 kb on + strand at 2174.514 kb on + strand at 2174.514 kb on + strand at 2174.515 kb on - strand at 2174.515 kb on - strand at 2174.515 kb on - strand at 2174.515 kb on - strand at 2174.515 kb on - strand at 2174.515 kb on - strand at 2174.515 kb on - strand at 2174.515 kb on - strand at 2174.515 kb on - strand at 2174.515 kb on - strand at 2174.515 kb on - strand at 2174.515 kb on - strand at 2174.515 kb on - strand at 2174.515 kb on - strand at 2174.515 kb on - strand at 2174.515 kb on - strand at 2174.515 kb on - strand at 2174.516 kb on + strand at 2174.516 kb on + strand at 2174.516 kb on + strand at 2174.517 kb on - strand at 2174.517 kb on - strand at 2174.517 kb on - strand at 2174.517 kb on - strand at 2174.517 kb on - strand at 2174.517 kb on - strand at 2174.517 kb on - strand at 2174.517 kb on - strand at 2174.518 kb on + strand at 2174.518 kb on + strand at 2174.518 kb on + strand at 2174.518 kb on + strand at 2174.518 kb on + strand at 2174.518 kb on + strand at 2174.518 kb on + strand at 2174.518 kb on + strand at 2174.519 kb on - strand at 2174.519 kb on - strand at 2174.519 kb on - strand at 2174.519 kb on - strand at 2174.519 kb on - strand at 2174.519 kb on - strand at 2174.529 kb on + strand at 2174.529 kb on + strand at 2174.529 kb on + strand at 2174.582 kb on + strand at 2174.583 kb on - strand at 2174.583 kb on - strand at 2174.597 kb on - strand at 2174.943 kb on + strand, within Pf1N1B4_1901 at 2174.943 kb on + strand, within Pf1N1B4_1901 at 2174.943 kb on + strand, within Pf1N1B4_1901 at 2174.943 kb on + strand, within Pf1N1B4_1901 at 2174.943 kb on + strand, within Pf1N1B4_1901 at 2174.943 kb on + strand, within Pf1N1B4_1901 at 2174.943 kb on + strand, within Pf1N1B4_1901 at 2174.943 kb on + strand, within Pf1N1B4_1901 at 2174.944 kb on - strand, within Pf1N1B4_1901 at 2174.944 kb on - strand, within Pf1N1B4_1901 at 2174.944 kb on - strand, within Pf1N1B4_1901 at 2175.080 kb on - strand, within Pf1N1B4_1901 at 2175.203 kb on + strand at 2175.204 kb on - strand at 2175.207 kb on - strand at 2175.207 kb on - strand at 2175.207 kb on - strand at 2175.244 kb on - strand at 2175.244 kb on - strand at 2175.276 kb on + strand at 2175.277 kb on - strand at 2175.373 kb on + strand, within Pf1N1B4_1902 at 2175.402 kb on + strand, within Pf1N1B4_1902 at 2175.402 kb on + strand, within Pf1N1B4_1902 at 2175.402 kb on + strand, within Pf1N1B4_1902 at 2175.402 kb on + strand, within Pf1N1B4_1902 at 2175.402 kb on + strand, within Pf1N1B4_1902 at 2175.402 kb on + strand, within Pf1N1B4_1902 at 2175.402 kb on + strand, within Pf1N1B4_1902 at 2175.402 kb on + strand, within Pf1N1B4_1902 at 2175.402 kb on + strand, within Pf1N1B4_1902 at 2175.402 kb on + strand, within Pf1N1B4_1902 at 2175.403 kb on - strand, within Pf1N1B4_1902 at 2175.403 kb on - strand, within Pf1N1B4_1902 at 2175.403 kb on - strand, within Pf1N1B4_1902 at 2175.403 kb on - strand, within Pf1N1B4_1902 at 2175.403 kb on - strand, within Pf1N1B4_1902 at 2175.403 kb on - strand, within Pf1N1B4_1902 at 2175.403 kb on - strand, within Pf1N1B4_1902 at 2175.403 kb on - strand at 2175.403 kb on - strand, within Pf1N1B4_1902 at 2175.403 kb on - strand, within Pf1N1B4_1902 at 2175.403 kb on - strand, within Pf1N1B4_1902 at 2175.403 kb on - strand at 2175.406 kb on - strand, within Pf1N1B4_1902 at 2175.469 kb on + strand, within Pf1N1B4_1902 at 2175.537 kb on + strand, within Pf1N1B4_1902 at 2175.537 kb on + strand, within Pf1N1B4_1902 at 2175.537 kb on + strand, within Pf1N1B4_1902 at 2175.538 kb on - strand, within Pf1N1B4_1902 at 2175.538 kb on - strand, within Pf1N1B4_1902 at 2175.538 kb on - strand, within Pf1N1B4_1902 at 2175.619 kb on + strand, within Pf1N1B4_1902 at 2175.619 kb on + strand, within Pf1N1B4_1902 at 2175.619 kb on + strand, within Pf1N1B4_1902 at 2175.619 kb on + strand, within Pf1N1B4_1902 at 2175.619 kb on + strand, within Pf1N1B4_1902 at 2175.619 kb on + strand, within Pf1N1B4_1902 at 2175.620 kb on - strand, within Pf1N1B4_1902 at 2175.701 kb on + strand, within Pf1N1B4_1902 at 2175.702 kb on - strand, within Pf1N1B4_1902 at 2175.901 kb on - strand, within Pf1N1B4_1902 at 2176.055 kb on - strand, within Pf1N1B4_1902 at 2176.055 kb on - strand, within Pf1N1B4_1902
Per-strain Table
Position Strand Gene LocusTag Fraction Cytosine (N) remove 2,173,549 + +0.9 2,173,550 - -0.9 2,173,568 + +0.3 2,173,568 + +1.6 2,173,569 - -0.7 2,173,620 - -0.2 2,173,696 - -0.1 2,173,696 - -0.7 2,173,696 - +0.3 2,173,705 - +0.3 2,173,881 + Pf1N1B4_1899 0.38 +0.0 2,173,882 - Pf1N1B4_1899 0.38 +0.9 2,173,882 - Pf1N1B4_1899 0.38 +0.9 2,173,914 - Pf1N1B4_1899 0.46 +2.9 2,173,941 - Pf1N1B4_1899 0.52 +0.9 2,174,056 + Pf1N1B4_1899 0.81 +0.7 2,174,056 + Pf1N1B4_1899 0.81 +1.3 2,174,056 + Pf1N1B4_1899 0.81 +1.3 2,174,056 + Pf1N1B4_1899 0.81 -0.4 2,174,057 - Pf1N1B4_1899 0.81 +0.3 2,174,057 - Pf1N1B4_1899 0.81 -2.0 2,174,057 - Pf1N1B4_1899 0.81 -0.1 2,174,057 - Pf1N1B4_1899 0.81 -0.1 2,174,228 + Pf1N1B4_1900 0.29 -0.9 2,174,228 + Pf1N1B4_1900 0.29 +1.8 2,174,228 + Pf1N1B4_1900 0.29 +0.9 2,174,229 - Pf1N1B4_1900 0.30 +1.6 2,174,229 - Pf1N1B4_1900 0.30 +1.3 2,174,267 + Pf1N1B4_1900 0.41 -0.2 2,174,267 + Pf1N1B4_1900 0.41 -0.6 2,174,267 + Pf1N1B4_1900 0.41 -0.0 2,174,268 - Pf1N1B4_1900 0.42 +0.3 2,174,268 - Pf1N1B4_1900 0.42 +0.1 2,174,268 - Pf1N1B4_1900 0.42 +1.0 2,174,280 + Pf1N1B4_1900 0.45 -0.7 2,174,280 + Pf1N1B4_1900 0.45 +0.0 2,174,280 + Pf1N1B4_1900 0.45 +1.3 2,174,280 + Pf1N1B4_1900 0.45 -0.1 2,174,280 + Pf1N1B4_1900 0.45 +1.6 2,174,280 + Pf1N1B4_1900 0.45 +0.4 2,174,280 + Pf1N1B4_1900 0.45 +1.2 2,174,280 + Pf1N1B4_1900 0.45 +2.1 2,174,280 + Pf1N1B4_1900 0.45 +0.4 2,174,280 + Pf1N1B4_1900 0.45 +0.9 2,174,280 + Pf1N1B4_1900 0.45 -0.2 2,174,280 + Pf1N1B4_1900 0.45 +0.7 2,174,280 + Pf1N1B4_1900 0.45 -0.1 2,174,281 - Pf1N1B4_1900 0.46 -0.4 2,174,281 - Pf1N1B4_1900 0.46 -1.3 2,174,281 - Pf1N1B4_1900 0.46 -1.4 2,174,281 - Pf1N1B4_1900 0.46 +1.5 2,174,281 - Pf1N1B4_1900 0.46 -0.2 2,174,281 - Pf1N1B4_1900 0.46 +0.9 2,174,281 - Pf1N1B4_1900 0.46 +0.9 2,174,281 - Pf1N1B4_1900 0.46 +1.2 2,174,281 - Pf1N1B4_1900 0.46 +0.3 2,174,281 - Pf1N1B4_1900 0.46 -1.9 2,174,281 - Pf1N1B4_1900 0.46 -0.0 2,174,281 - Pf1N1B4_1900 0.46 -0.2 2,174,281 - Pf1N1B4_1900 0.46 +0.5 2,174,424 + Pf1N1B4_1900 0.89 +1.3 2,174,424 + Pf1N1B4_1900 0.89 +0.4 2,174,424 + Pf1N1B4_1900 0.89 -0.3 2,174,424 + Pf1N1B4_1900 0.89 +1.8 2,174,425 - Pf1N1B4_1900 0.90 +1.0 2,174,425 - Pf1N1B4_1900 0.90 +0.6 2,174,425 - Pf1N1B4_1900 0.90 -0.4 2,174,439 + +0.8 2,174,470 + -0.5 2,174,475 + +1.9 2,174,475 + +1.5 2,174,475 + +0.3 2,174,475 + -0.3 2,174,475 + +0.8 2,174,476 - +0.3 2,174,476 - +2.6 2,174,476 - -1.7 2,174,476 - -0.1 2,174,476 - +1.1 2,174,476 - +0.3 2,174,476 - +1.0 2,174,514 + +1.1 2,174,514 + -0.1 2,174,514 + +0.0 2,174,514 + +3.2 2,174,514 + +0.6 2,174,514 + +1.0 2,174,514 + -0.1 2,174,514 + -0.7 2,174,514 + +0.6 2,174,514 + -0.8 2,174,515 - -0.5 2,174,515 - +1.3 2,174,515 - +0.5 2,174,515 - +0.9 2,174,515 - +0.9 2,174,515 - +0.9 2,174,515 - -0.7 2,174,515 - +0.7 2,174,515 - -0.1 2,174,515 - +0.5 2,174,515 - +1.3 2,174,515 - +1.3 2,174,515 - -0.7 2,174,515 - -0.1 2,174,515 - +1.5 2,174,515 - +1.4 2,174,515 - +0.1 2,174,516 + +0.7 2,174,516 + -0.7 2,174,516 + +0.3 2,174,517 - -0.9 2,174,517 - -0.7 2,174,517 - +0.3 2,174,517 - -0.3 2,174,517 - +1.3 2,174,517 - +0.5 2,174,517 - -0.7 2,174,517 - +0.0 2,174,518 + +1.2 2,174,518 + +0.8 2,174,518 + -0.1 2,174,518 + -0.1 2,174,518 + +0.3 2,174,518 + +1.8 2,174,518 + -0.1 2,174,518 + +0.6 2,174,519 - +0.9 2,174,519 - +1.0 2,174,519 - +0.0 2,174,519 - -0.4 2,174,519 - -0.7 2,174,519 - +1.6 2,174,529 + +0.4 2,174,529 + +1.6 2,174,529 + -0.3 2,174,582 + +1.4 2,174,583 - +1.1 2,174,583 - +0.5 2,174,597 - +0.9 2,174,943 + Pf1N1B4_1901 0.60 +0.3 2,174,943 + Pf1N1B4_1901 0.60 +0.0 2,174,943 + Pf1N1B4_1901 0.60 -0.0 2,174,943 + Pf1N1B4_1901 0.60 -0.7 2,174,943 + Pf1N1B4_1901 0.60 -0.7 2,174,943 + Pf1N1B4_1901 0.60 -1.7 2,174,943 + Pf1N1B4_1901 0.60 +0.4 2,174,943 + Pf1N1B4_1901 0.60 -0.4 2,174,944 - Pf1N1B4_1901 0.60 -0.3 2,174,944 - Pf1N1B4_1901 0.60 -1.7 2,174,944 - Pf1N1B4_1901 0.60 +0.6 2,175,080 - Pf1N1B4_1901 0.81 -0.1 2,175,203 + -0.2 2,175,204 - +1.9 2,175,207 - -0.7 2,175,207 - -0.7 2,175,207 - -1.4 2,175,244 - -1.7 2,175,244 - +0.9 2,175,276 + +0.9 2,175,277 - +1.5 2,175,373 + Pf1N1B4_1902 0.10 +1.7 2,175,402 + Pf1N1B4_1902 0.13 +1.2 2,175,402 + Pf1N1B4_1902 0.13 -0.7 2,175,402 + Pf1N1B4_1902 0.13 -1.1 2,175,402 + Pf1N1B4_1902 0.13 -0.4 2,175,402 + Pf1N1B4_1902 0.13 +0.5 2,175,402 + Pf1N1B4_1902 0.13 +1.0 2,175,402 + Pf1N1B4_1902 0.13 -0.1 2,175,402 + Pf1N1B4_1902 0.13 +1.8 2,175,402 + Pf1N1B4_1902 0.13 +0.6 2,175,402 + Pf1N1B4_1902 0.13 -0.7 2,175,403 - Pf1N1B4_1902 0.13 +1.5 2,175,403 - Pf1N1B4_1902 0.13 +1.0 2,175,403 - Pf1N1B4_1902 0.13 +0.3 2,175,403 - Pf1N1B4_1902 0.13 +1.5 2,175,403 - Pf1N1B4_1902 0.13 +0.9 2,175,403 - Pf1N1B4_1902 0.13 +0.9 2,175,403 - Pf1N1B4_1902 0.13 -0.3 2,175,403 - +1.6 2,175,403 - Pf1N1B4_1902 0.13 +2.5 2,175,403 - Pf1N1B4_1902 0.13 -1.4 2,175,403 - Pf1N1B4_1902 0.13 +0.9 2,175,403 - -0.7 2,175,406 - Pf1N1B4_1902 0.14 -0.7 2,175,469 + Pf1N1B4_1902 0.20 +1.9 2,175,537 + Pf1N1B4_1902 0.28 +1.6 2,175,537 + Pf1N1B4_1902 0.28 +0.5 2,175,537 + Pf1N1B4_1902 0.28 +1.2 2,175,538 - Pf1N1B4_1902 0.28 -0.3 2,175,538 - Pf1N1B4_1902 0.28 +1.6 2,175,538 - Pf1N1B4_1902 0.28 +2.5 2,175,619 + Pf1N1B4_1902 0.36 +2.1 2,175,619 + Pf1N1B4_1902 0.36 +1.5 2,175,619 + Pf1N1B4_1902 0.36 +1.5 2,175,619 + Pf1N1B4_1902 0.36 +0.7 2,175,619 + Pf1N1B4_1902 0.36 -1.9 2,175,619 + Pf1N1B4_1902 0.36 -0.1 2,175,620 - Pf1N1B4_1902 0.36 -1.7 2,175,701 + Pf1N1B4_1902 0.45 -1.4 2,175,702 - Pf1N1B4_1902 0.45 +0.4 2,175,901 - Pf1N1B4_1902 0.66 +0.3 2,176,055 - Pf1N1B4_1902 0.82 -0.3 2,176,055 - Pf1N1B4_1902 0.82 +0.7
Or see this region's nucleotide sequence