Experiment: Recovery broth with Sulfaquinoxaline 0.01 mM and Trimethoprim 0.003 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS17310 and BBR_RS17315 are separated by 251 nucleotides BBR_RS17315 and BBR_RS17320 are separated by 3 nucleotides BBR_RS17320 and BBR_RS17330 are separated by 174 nucleotides
BBR_RS17310: BBR_RS17310 - D-2-hydroxyacid dehydrogenase, at 1,660,687 to 1,661,673
_RS17310
BBR_RS17315: BBR_RS17315 - MFS transporter, at 1,661,925 to 1,663,385
_RS17315
BBR_RS17320: BBR_RS17320 - response regulator, at 1,663,389 to 1,663,853
_RS17320
BBR_RS17330: BBR_RS17330 - replication-associated recombination protein A, at 1,664,028 to 1,665,407
_RS17330
Position (kb)
1661
1662
1663
1664 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1660.936 kb on + strand, within BBR_RS17310 at 1660.954 kb on + strand, within BBR_RS17310 at 1660.975 kb on - strand, within BBR_RS17310 at 1660.987 kb on - strand, within BBR_RS17310 at 1661.008 kb on - strand, within BBR_RS17310 at 1661.014 kb on + strand, within BBR_RS17310 at 1661.014 kb on + strand, within BBR_RS17310 at 1661.015 kb on - strand, within BBR_RS17310 at 1661.022 kb on - strand, within BBR_RS17310 at 1661.069 kb on + strand, within BBR_RS17310 at 1661.153 kb on + strand, within BBR_RS17310 at 1661.153 kb on + strand, within BBR_RS17310 at 1661.161 kb on - strand, within BBR_RS17310 at 1661.161 kb on - strand, within BBR_RS17310 at 1661.161 kb on - strand, within BBR_RS17310 at 1661.161 kb on - strand, within BBR_RS17310 at 1661.161 kb on - strand, within BBR_RS17310 at 1661.161 kb on - strand, within BBR_RS17310 at 1661.161 kb on - strand, within BBR_RS17310 at 1661.163 kb on + strand, within BBR_RS17310 at 1661.173 kb on + strand, within BBR_RS17310 at 1661.173 kb on + strand, within BBR_RS17310 at 1661.188 kb on + strand, within BBR_RS17310 at 1661.209 kb on - strand, within BBR_RS17310 at 1661.219 kb on - strand, within BBR_RS17310 at 1661.219 kb on - strand, within BBR_RS17310 at 1661.223 kb on + strand, within BBR_RS17310 at 1661.223 kb on + strand, within BBR_RS17310 at 1661.223 kb on + strand, within BBR_RS17310 at 1661.235 kb on + strand, within BBR_RS17310 at 1661.235 kb on + strand, within BBR_RS17310 at 1661.243 kb on - strand, within BBR_RS17310 at 1661.251 kb on - strand, within BBR_RS17310 at 1661.255 kb on - strand, within BBR_RS17310 at 1661.279 kb on - strand, within BBR_RS17310 at 1661.279 kb on - strand, within BBR_RS17310 at 1661.306 kb on + strand, within BBR_RS17310 at 1661.314 kb on - strand, within BBR_RS17310 at 1661.383 kb on + strand, within BBR_RS17310 at 1661.401 kb on + strand, within BBR_RS17310 at 1661.410 kb on + strand, within BBR_RS17310 at 1661.417 kb on - strand, within BBR_RS17310 at 1661.455 kb on - strand, within BBR_RS17310 at 1661.457 kb on + strand, within BBR_RS17310 at 1661.460 kb on - strand, within BBR_RS17310 at 1661.505 kb on - strand, within BBR_RS17310 at 1661.544 kb on + strand, within BBR_RS17310 at 1661.594 kb on + strand at 1661.722 kb on + strand at 1661.731 kb on - strand at 1661.731 kb on - strand at 1661.731 kb on - strand at 1661.746 kb on + strand at 1661.753 kb on + strand at 1661.753 kb on + strand at 1661.753 kb on + strand at 1661.753 kb on + strand at 1661.753 kb on + strand at 1661.753 kb on + strand at 1661.753 kb on - strand at 1661.761 kb on - strand at 1661.761 kb on - strand at 1661.761 kb on - strand at 1661.761 kb on - strand at 1661.761 kb on - strand at 1661.761 kb on - strand at 1661.785 kb on + strand at 1661.796 kb on - strand at 1661.796 kb on - strand at 1661.859 kb on + strand at 1661.876 kb on - strand at 1661.882 kb on + strand at 1661.929 kb on - strand at 1661.939 kb on - strand at 1661.972 kb on - strand at 1662.036 kb on + strand at 1662.038 kb on + strand at 1662.066 kb on + strand at 1662.080 kb on - strand, within BBR_RS17315 at 1662.208 kb on + strand, within BBR_RS17315 at 1662.245 kb on + strand, within BBR_RS17315 at 1662.253 kb on - strand, within BBR_RS17315 at 1662.283 kb on - strand, within BBR_RS17315 at 1662.312 kb on + strand, within BBR_RS17315 at 1662.323 kb on + strand, within BBR_RS17315 at 1662.368 kb on + strand, within BBR_RS17315 at 1662.376 kb on - strand, within BBR_RS17315 at 1662.411 kb on + strand, within BBR_RS17315 at 1662.499 kb on - strand, within BBR_RS17315 at 1662.499 kb on - strand, within BBR_RS17315 at 1662.532 kb on - strand, within BBR_RS17315 at 1662.558 kb on + strand, within BBR_RS17315 at 1662.574 kb on + strand, within BBR_RS17315 at 1662.574 kb on + strand, within BBR_RS17315 at 1662.594 kb on - strand, within BBR_RS17315 at 1662.729 kb on + strand, within BBR_RS17315 at 1662.735 kb on - strand, within BBR_RS17315 at 1662.735 kb on - strand, within BBR_RS17315 at 1662.770 kb on + strand, within BBR_RS17315 at 1662.813 kb on + strand, within BBR_RS17315 at 1662.820 kb on + strand, within BBR_RS17315 at 1662.820 kb on + strand, within BBR_RS17315 at 1662.880 kb on + strand, within BBR_RS17315 at 1662.890 kb on + strand, within BBR_RS17315 at 1662.897 kb on + strand, within BBR_RS17315 at 1662.903 kb on - strand, within BBR_RS17315 at 1662.910 kb on - strand, within BBR_RS17315 at 1662.991 kb on + strand, within BBR_RS17315 at 1662.997 kb on + strand, within BBR_RS17315 at 1663.021 kb on - strand, within BBR_RS17315 at 1663.021 kb on - strand, within BBR_RS17315 at 1663.028 kb on + strand, within BBR_RS17315 at 1663.041 kb on - strand, within BBR_RS17315 at 1663.073 kb on + strand, within BBR_RS17315 at 1663.081 kb on - strand, within BBR_RS17315 at 1663.081 kb on - strand, within BBR_RS17315 at 1663.088 kb on - strand, within BBR_RS17315 at 1663.115 kb on + strand, within BBR_RS17315 at 1663.126 kb on + strand, within BBR_RS17315 at 1663.168 kb on + strand, within BBR_RS17315 at 1663.168 kb on + strand, within BBR_RS17315 at 1663.197 kb on + strand, within BBR_RS17315 at 1663.197 kb on + strand, within BBR_RS17315 at 1663.197 kb on + strand, within BBR_RS17315 at 1663.197 kb on + strand, within BBR_RS17315 at 1663.224 kb on + strand, within BBR_RS17315 at 1663.232 kb on - strand, within BBR_RS17315 at 1663.255 kb on - strand at 1663.307 kb on + strand at 1663.360 kb on - strand at 1663.458 kb on - strand, within BBR_RS17320 at 1663.508 kb on + strand, within BBR_RS17320 at 1663.517 kb on - strand, within BBR_RS17320 at 1663.518 kb on + strand, within BBR_RS17320 at 1663.526 kb on - strand, within BBR_RS17320 at 1663.527 kb on + strand, within BBR_RS17320 at 1663.564 kb on + strand, within BBR_RS17320 at 1663.646 kb on - strand, within BBR_RS17320 at 1663.646 kb on - strand, within BBR_RS17320 at 1663.649 kb on - strand, within BBR_RS17320 at 1663.671 kb on - strand, within BBR_RS17320 at 1663.676 kb on - strand, within BBR_RS17320 at 1663.676 kb on - strand, within BBR_RS17320 at 1663.732 kb on + strand, within BBR_RS17320 at 1663.732 kb on + strand, within BBR_RS17320 at 1663.740 kb on + strand, within BBR_RS17320 at 1663.740 kb on - strand, within BBR_RS17320 at 1663.762 kb on - strand, within BBR_RS17320 at 1663.792 kb on - strand, within BBR_RS17320 at 1663.792 kb on - strand, within BBR_RS17320 at 1663.792 kb on - strand, within BBR_RS17320 at 1663.802 kb on - strand, within BBR_RS17320 at 1663.802 kb on - strand, within BBR_RS17320 at 1663.808 kb on + strand at 1663.833 kb on - strand at 1663.837 kb on - strand at 1663.864 kb on + strand at 1663.864 kb on + strand at 1663.894 kb on - strand at 1663.923 kb on - strand at 1663.941 kb on - strand at 1663.970 kb on + strand at 1663.973 kb on - strand at 1664.019 kb on + strand at 1664.020 kb on - strand at 1664.022 kb on - strand at 1664.057 kb on - strand at 1664.067 kb on + strand at 1664.136 kb on + strand at 1664.196 kb on + strand, within BBR_RS17330 at 1664.210 kb on - strand, within BBR_RS17330 at 1664.212 kb on - strand, within BBR_RS17330 at 1664.222 kb on - strand, within BBR_RS17330 at 1664.256 kb on + strand, within BBR_RS17330 at 1664.256 kb on + strand, within BBR_RS17330 at 1664.256 kb on + strand, within BBR_RS17330 at 1664.256 kb on + strand, within BBR_RS17330 at 1664.323 kb on + strand, within BBR_RS17330 at 1664.331 kb on - strand, within BBR_RS17330 at 1664.338 kb on + strand, within BBR_RS17330
Per-strain Table
Position Strand Gene LocusTag Fraction Recovery broth with Sulfaquinoxaline 0.01 mM and Trimethoprim 0.003 mM remove 1,660,936 + BBR_RS17310 0.25 +0.5 1,660,954 + BBR_RS17310 0.27 +0.2 1,660,975 - BBR_RS17310 0.29 +0.4 1,660,987 - BBR_RS17310 0.30 +0.1 1,661,008 - BBR_RS17310 0.33 +1.1 1,661,014 + BBR_RS17310 0.33 -1.2 1,661,014 + BBR_RS17310 0.33 +0.5 1,661,015 - BBR_RS17310 0.33 -0.3 1,661,022 - BBR_RS17310 0.34 +0.1 1,661,069 + BBR_RS17310 0.39 +0.0 1,661,153 + BBR_RS17310 0.47 +0.3 1,661,153 + BBR_RS17310 0.47 +1.6 1,661,161 - BBR_RS17310 0.48 -0.4 1,661,161 - BBR_RS17310 0.48 +0.6 1,661,161 - BBR_RS17310 0.48 +0.3 1,661,161 - BBR_RS17310 0.48 +0.6 1,661,161 - BBR_RS17310 0.48 +0.5 1,661,161 - BBR_RS17310 0.48 -0.5 1,661,161 - BBR_RS17310 0.48 -0.3 1,661,163 + BBR_RS17310 0.48 +1.6 1,661,173 + BBR_RS17310 0.49 -1.0 1,661,173 + BBR_RS17310 0.49 -0.4 1,661,188 + BBR_RS17310 0.51 -0.2 1,661,209 - BBR_RS17310 0.53 +0.1 1,661,219 - BBR_RS17310 0.54 -0.6 1,661,219 - BBR_RS17310 0.54 +0.5 1,661,223 + BBR_RS17310 0.54 +0.3 1,661,223 + BBR_RS17310 0.54 +0.5 1,661,223 + BBR_RS17310 0.54 -1.1 1,661,235 + BBR_RS17310 0.56 -0.5 1,661,235 + BBR_RS17310 0.56 -0.2 1,661,243 - BBR_RS17310 0.56 -0.6 1,661,251 - BBR_RS17310 0.57 -0.2 1,661,255 - BBR_RS17310 0.58 -0.4 1,661,279 - BBR_RS17310 0.60 +0.0 1,661,279 - BBR_RS17310 0.60 -0.2 1,661,306 + BBR_RS17310 0.63 +0.2 1,661,314 - BBR_RS17310 0.64 +0.3 1,661,383 + BBR_RS17310 0.71 -1.1 1,661,401 + BBR_RS17310 0.72 -2.4 1,661,410 + BBR_RS17310 0.73 +0.6 1,661,417 - BBR_RS17310 0.74 +0.6 1,661,455 - BBR_RS17310 0.78 -0.6 1,661,457 + BBR_RS17310 0.78 +0.5 1,661,460 - BBR_RS17310 0.78 +0.5 1,661,505 - BBR_RS17310 0.83 +0.1 1,661,544 + BBR_RS17310 0.87 -0.2 1,661,594 + +0.1 1,661,722 + -0.9 1,661,731 - +0.6 1,661,731 - +0.5 1,661,731 - +1.0 1,661,746 + -0.7 1,661,753 + -0.9 1,661,753 + -0.6 1,661,753 + -0.1 1,661,753 + -0.1 1,661,753 + -0.5 1,661,753 + -0.7 1,661,753 - +0.5 1,661,761 - +0.0 1,661,761 - -1.8 1,661,761 - +0.2 1,661,761 - +0.7 1,661,761 - -0.0 1,661,761 - +0.1 1,661,785 + +0.2 1,661,796 - +0.1 1,661,796 - +0.8 1,661,859 + +1.2 1,661,876 - -0.5 1,661,882 + -0.2 1,661,929 - +0.6 1,661,939 - -0.1 1,661,972 - +0.2 1,662,036 + -0.1 1,662,038 + +0.3 1,662,066 + +1.5 1,662,080 - BBR_RS17315 0.11 +0.0 1,662,208 + BBR_RS17315 0.19 +1.4 1,662,245 + BBR_RS17315 0.22 -0.8 1,662,253 - BBR_RS17315 0.22 -1.2 1,662,283 - BBR_RS17315 0.25 +1.6 1,662,312 + BBR_RS17315 0.26 +0.4 1,662,323 + BBR_RS17315 0.27 +0.4 1,662,368 + BBR_RS17315 0.30 +1.3 1,662,376 - BBR_RS17315 0.31 -0.1 1,662,411 + BBR_RS17315 0.33 +0.6 1,662,499 - BBR_RS17315 0.39 +1.0 1,662,499 - BBR_RS17315 0.39 +1.0 1,662,532 - BBR_RS17315 0.42 +1.4 1,662,558 + BBR_RS17315 0.43 +1.1 1,662,574 + BBR_RS17315 0.44 +0.6 1,662,574 + BBR_RS17315 0.44 +0.9 1,662,594 - BBR_RS17315 0.46 +0.7 1,662,729 + BBR_RS17315 0.55 +0.8 1,662,735 - BBR_RS17315 0.55 +1.0 1,662,735 - BBR_RS17315 0.55 +0.3 1,662,770 + BBR_RS17315 0.58 +0.5 1,662,813 + BBR_RS17315 0.61 +0.6 1,662,820 + BBR_RS17315 0.61 +1.0 1,662,820 + BBR_RS17315 0.61 +0.5 1,662,880 + BBR_RS17315 0.65 +0.6 1,662,890 + BBR_RS17315 0.66 +0.2 1,662,897 + BBR_RS17315 0.67 -0.4 1,662,903 - BBR_RS17315 0.67 +1.7 1,662,910 - BBR_RS17315 0.67 -0.3 1,662,991 + BBR_RS17315 0.73 +0.8 1,662,997 + BBR_RS17315 0.73 -0.7 1,663,021 - BBR_RS17315 0.75 +0.6 1,663,021 - BBR_RS17315 0.75 +1.1 1,663,028 + BBR_RS17315 0.75 +0.2 1,663,041 - BBR_RS17315 0.76 +1.1 1,663,073 + BBR_RS17315 0.79 +0.4 1,663,081 - BBR_RS17315 0.79 +0.6 1,663,081 - BBR_RS17315 0.79 -0.3 1,663,088 - BBR_RS17315 0.80 +1.3 1,663,115 + BBR_RS17315 0.81 +1.5 1,663,126 + BBR_RS17315 0.82 +0.6 1,663,168 + BBR_RS17315 0.85 +0.8 1,663,168 + BBR_RS17315 0.85 +0.5 1,663,197 + BBR_RS17315 0.87 +0.8 1,663,197 + BBR_RS17315 0.87 +0.0 1,663,197 + BBR_RS17315 0.87 +1.0 1,663,197 + BBR_RS17315 0.87 +0.2 1,663,224 + BBR_RS17315 0.89 +0.5 1,663,232 - BBR_RS17315 0.89 +1.0 1,663,255 - +0.1 1,663,307 + +3.3 1,663,360 - +0.9 1,663,458 - BBR_RS17320 0.15 +0.4 1,663,508 + BBR_RS17320 0.26 +1.0 1,663,517 - BBR_RS17320 0.28 +0.7 1,663,518 + BBR_RS17320 0.28 +0.9 1,663,526 - BBR_RS17320 0.29 +0.4 1,663,527 + BBR_RS17320 0.30 -0.6 1,663,564 + BBR_RS17320 0.38 -0.4 1,663,646 - BBR_RS17320 0.55 +0.3 1,663,646 - BBR_RS17320 0.55 -0.1 1,663,649 - BBR_RS17320 0.56 +0.1 1,663,671 - BBR_RS17320 0.61 +0.1 1,663,676 - BBR_RS17320 0.62 -0.6 1,663,676 - BBR_RS17320 0.62 +0.3 1,663,732 + BBR_RS17320 0.74 -0.4 1,663,732 + BBR_RS17320 0.74 +0.1 1,663,740 + BBR_RS17320 0.75 +0.2 1,663,740 - BBR_RS17320 0.75 +0.9 1,663,762 - BBR_RS17320 0.80 +0.3 1,663,792 - BBR_RS17320 0.87 +0.8 1,663,792 - BBR_RS17320 0.87 -0.4 1,663,792 - BBR_RS17320 0.87 +0.5 1,663,802 - BBR_RS17320 0.89 -0.1 1,663,802 - BBR_RS17320 0.89 +0.4 1,663,808 + -0.7 1,663,833 - +0.3 1,663,837 - -0.3 1,663,864 + -1.1 1,663,864 + +0.4 1,663,894 - +0.3 1,663,923 - +1.1 1,663,941 - +0.3 1,663,970 + +0.4 1,663,973 - -0.1 1,664,019 + -0.3 1,664,020 - +0.3 1,664,022 - -0.1 1,664,057 - +1.3 1,664,067 + +0.8 1,664,136 + +0.2 1,664,196 + BBR_RS17330 0.12 +0.8 1,664,210 - BBR_RS17330 0.13 -0.4 1,664,212 - BBR_RS17330 0.13 -0.9 1,664,222 - BBR_RS17330 0.14 +0.4 1,664,256 + BBR_RS17330 0.17 -0.1 1,664,256 + BBR_RS17330 0.17 +0.5 1,664,256 + BBR_RS17330 0.17 -0.5 1,664,256 + BBR_RS17330 0.17 +0.7 1,664,323 + BBR_RS17330 0.21 -0.5 1,664,331 - BBR_RS17330 0.22 +0.6 1,664,338 + BBR_RS17330 0.22 -0.3
Or see this region's nucleotide sequence