Experiment: L-Malic (C) and no Nitrogen; Wolfe's minerals without nitroloacetic acid; 3 days
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AZOBR_RS24530 and AZOBR_RS24535 overlap by 7 nucleotides AZOBR_RS24535 and AZOBR_RS24540 are separated by 13 nucleotides AZOBR_RS24540 and AZOBR_RS24545 overlap by 4 nucleotides
AZOBR_RS24530: AZOBR_RS24530 - formylmethanofuran dehydrogenase, at 561,236 to 562,507
_RS24530
AZOBR_RS24535: AZOBR_RS24535 - formylmethanofuran dehydrogenase subunit A, at 562,501 to 564,162
_RS24535
AZOBR_RS24540: AZOBR_RS24540 - formylmethanofuran--tetrahydromethanopterin formyltransferase, at 564,176 to 565,075
_RS24540
AZOBR_RS24545: AZOBR_RS24545 - putative formylmethanofuran dehydrogenase subunit C, at 565,072 to 565,896
_RS24545
Position (kb) 562
563
564
565 Strain fitness (log2 ratio) -1
0
1 at 562.532 kb on - strand at 562.532 kb on - strand at 562.837 kb on + strand, within AZOBR_RS24535 at 562.838 kb on - strand, within AZOBR_RS24535 at 563.006 kb on - strand, within AZOBR_RS24535 at 563.006 kb on - strand, within AZOBR_RS24535 at 563.084 kb on - strand, within AZOBR_RS24535 at 563.668 kb on + strand, within AZOBR_RS24535 at 563.780 kb on - strand, within AZOBR_RS24535 at 563.888 kb on - strand, within AZOBR_RS24535 at 564.019 kb on + strand at 564.020 kb on - strand at 564.540 kb on - strand, within AZOBR_RS24540 at 564.587 kb on + strand, within AZOBR_RS24540 at 564.806 kb on + strand, within AZOBR_RS24540 at 564.806 kb on + strand, within AZOBR_RS24540 at 564.806 kb on + strand, within AZOBR_RS24540 at 564.806 kb on + strand, within AZOBR_RS24540 at 564.806 kb on + strand, within AZOBR_RS24540 at 564.806 kb on + strand, within AZOBR_RS24540 at 564.806 kb on + strand, within AZOBR_RS24540 at 564.807 kb on - strand, within AZOBR_RS24540
Per-strain Table
Position Strand Gene LocusTag Fraction L-Malic (C) and no Nitrogen; Wolfe's minerals without nitroloacetic acid; 3 days remove 562,532 - +0.5 562,532 - +0.5 562,837 + AZOBR_RS24535 0.20 -0.2 562,838 - AZOBR_RS24535 0.20 -0.8 563,006 - AZOBR_RS24535 0.30 +0.8 563,006 - AZOBR_RS24535 0.30 -0.1 563,084 - AZOBR_RS24535 0.35 +0.6 563,668 + AZOBR_RS24535 0.70 +0.5 563,780 - AZOBR_RS24535 0.77 +0.3 563,888 - AZOBR_RS24535 0.83 +0.3 564,019 + -0.4 564,020 - -0.3 564,540 - AZOBR_RS24540 0.40 -0.2 564,587 + AZOBR_RS24540 0.46 +0.5 564,806 + AZOBR_RS24540 0.70 +0.7 564,806 + AZOBR_RS24540 0.70 +0.5 564,806 + AZOBR_RS24540 0.70 +0.3 564,806 + AZOBR_RS24540 0.70 -1.2 564,806 + AZOBR_RS24540 0.70 -0.1 564,806 + AZOBR_RS24540 0.70 +0.4 564,806 + AZOBR_RS24540 0.70 +1.1 564,807 - AZOBR_RS24540 0.70 +1.0
Or see this region's nucleotide sequence