Strain Fitness in Azospirillum brasilense Sp245 around AZOBR_RS06240

Experiment: L-Malic (C) and no Nitrogen; Wolfe's minerals without nitroloacetic acid; 3 days

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntAZOBR_RS06230 and AZOBR_RS06235 are separated by 100 nucleotidesAZOBR_RS06235 and AZOBR_RS06240 are separated by 251 nucleotidesAZOBR_RS06240 and AZOBR_RS06245 are separated by 5 nucleotidesAZOBR_RS06245 and AZOBR_RS06250 are separated by 6 nucleotides AZOBR_RS06230: AZOBR_RS06230 - hypothetical protein, at 1,317,923 to 1,319,881 _RS06230 AZOBR_RS06235: AZOBR_RS06235 - hypothetical protein, at 1,319,982 to 1,320,269 _RS06235 AZOBR_RS06240: AZOBR_RS06240 - hypothetical protein, at 1,320,521 to 1,321,024 _RS06240 AZOBR_RS06245: AZOBR_RS06245 - pyrroline-5-carboxylate reductase, at 1,321,030 to 1,321,857 _RS06245 AZOBR_RS06250: AZOBR_RS06250 - membrane protein, at 1,321,864 to 1,322,346 _RS06250 Position (kb) 1320 1321 1322Strain fitness (log2 ratio) -1 0 1at 1319.709 kb on + strandat 1319.710 kb on - strandat 1319.997 kb on - strandat 1320.412 kb on + strandat 1320.412 kb on + strandat 1320.413 kb on - strandat 1320.674 kb on + strand, within AZOBR_RS06240at 1321.579 kb on + strand, within AZOBR_RS06245at 1321.580 kb on - strand, within AZOBR_RS06245at 1321.856 kb on - strandat 1321.856 kb on - strandat 1321.856 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Malic (C) and no Nitrogen; Wolfe's minerals without nitroloacetic acid; 3 days
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1,319,709 + +0.1
1,319,710 - +0.8
1,319,997 - -0.4
1,320,412 + +0.5
1,320,412 + -0.5
1,320,413 - -0.9
1,320,674 + AZOBR_RS06240 0.30 -0.6
1,321,579 + AZOBR_RS06245 0.66 -1.3
1,321,580 - AZOBR_RS06245 0.66 -0.6
1,321,856 - +0.9
1,321,856 - -0.6
1,321,856 - -0.3

Or see this region's nucleotide sequence