Experiment: Carbon source Sucrose 5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_1225 and Shewana3_1226 are separated by 144 nucleotides Shewana3_1226 and Shewana3_1227 are separated by 32 nucleotides Shewana3_1227 and Shewana3_1228 overlap by 4 nucleotides
Shewana3_1225: Shewana3_1225 - hypothetical protein (RefSeq), at 1,428,213 to 1,429,304
_1225
Shewana3_1226: Shewana3_1226 - hypothetical protein (RefSeq), at 1,429,449 to 1,430,570
_1226
Shewana3_1227: Shewana3_1227 - type IV pilus biogenesis/stability protein PilW (RefSeq), at 1,430,603 to 1,431,391
_1227
Shewana3_1228: Shewana3_1228 - hypothetical protein (RefSeq), at 1,431,388 to 1,432,515
_1228
Position (kb)
1429
1430
1431 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1428.805 kb on + strand, within Shewana3_1225 at 1428.845 kb on - strand, within Shewana3_1225 at 1428.944 kb on + strand, within Shewana3_1225 at 1428.952 kb on - strand, within Shewana3_1225 at 1429.108 kb on - strand, within Shewana3_1225 at 1429.217 kb on + strand at 1429.225 kb on - strand at 1429.225 kb on - strand at 1429.227 kb on + strand at 1429.230 kb on - strand at 1429.273 kb on + strand at 1429.359 kb on + strand at 1429.373 kb on - strand at 1429.740 kb on - strand, within Shewana3_1226 at 1429.796 kb on + strand, within Shewana3_1226 at 1429.827 kb on + strand, within Shewana3_1226 at 1430.022 kb on - strand, within Shewana3_1226 at 1430.136 kb on - strand, within Shewana3_1226 at 1430.224 kb on - strand, within Shewana3_1226 at 1430.230 kb on - strand, within Shewana3_1226 at 1430.278 kb on + strand, within Shewana3_1226 at 1430.329 kb on + strand, within Shewana3_1226 at 1430.329 kb on + strand, within Shewana3_1226 at 1430.337 kb on - strand, within Shewana3_1226 at 1430.821 kb on - strand, within Shewana3_1227 at 1430.925 kb on - strand, within Shewana3_1227 at 1431.499 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Carbon source Sucrose 5 mM remove 1,428,805 + Shewana3_1225 0.54 -0.9 1,428,845 - Shewana3_1225 0.58 +1.1 1,428,944 + Shewana3_1225 0.67 +0.7 1,428,952 - Shewana3_1225 0.68 +0.8 1,429,108 - Shewana3_1225 0.82 -0.3 1,429,217 + +0.9 1,429,225 - -0.1 1,429,225 - -0.3 1,429,227 + -0.8 1,429,230 - +0.9 1,429,273 + -3.1 1,429,359 + -0.2 1,429,373 - +0.1 1,429,740 - Shewana3_1226 0.26 -0.3 1,429,796 + Shewana3_1226 0.31 -0.4 1,429,827 + Shewana3_1226 0.34 -3.3 1,430,022 - Shewana3_1226 0.51 -2.2 1,430,136 - Shewana3_1226 0.61 -1.6 1,430,224 - Shewana3_1226 0.69 -0.9 1,430,230 - Shewana3_1226 0.70 -0.2 1,430,278 + Shewana3_1226 0.74 -0.7 1,430,329 + Shewana3_1226 0.78 -2.1 1,430,329 + Shewana3_1226 0.78 -0.9 1,430,337 - Shewana3_1226 0.79 -1.9 1,430,821 - Shewana3_1227 0.28 -1.4 1,430,925 - Shewana3_1227 0.41 +0.4 1,431,499 + +1.4
Or see this region's nucleotide sequence