Experiment: Carbon source Sucrose 5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_0440 and purH are separated by 105 nucleotides purH and zntR are separated by 336 nucleotides zntR and Shewana3_0443 are separated by 2 nucleotides
Shewana3_0440: Shewana3_0440 - phosphoribosylamine--glycine ligase (RefSeq), at 514,383 to 515,681
_0440
Shewana3_0441: purH - bifunctional phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase (RefSeq), at 515,787 to 517,385
purH
Shewana3_0442: zntR - zinc-responsive transcriptional regulator (RefSeq), at 517,722 to 518,195
zntR
Shewana3_0443: Shewana3_0443 - permease (RefSeq), at 518,198 to 519,691
_0443
Position (kb) 515
516
517
518 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 514.845 kb on + strand, within Shewana3_0440 at 514.853 kb on - strand, within Shewana3_0440 at 514.919 kb on + strand, within Shewana3_0440 at 514.999 kb on - strand, within Shewana3_0440 at 515.103 kb on - strand, within Shewana3_0440 at 515.209 kb on + strand, within Shewana3_0440 at 515.283 kb on + strand, within Shewana3_0440 at 515.291 kb on - strand, within Shewana3_0440 at 515.318 kb on - strand, within Shewana3_0440 at 515.352 kb on - strand, within Shewana3_0440 at 515.366 kb on + strand, within Shewana3_0440 at 515.374 kb on - strand, within Shewana3_0440 at 515.374 kb on - strand, within Shewana3_0440 at 515.425 kb on + strand, within Shewana3_0440 at 515.433 kb on - strand, within Shewana3_0440 at 515.462 kb on + strand, within Shewana3_0440 at 515.516 kb on + strand, within Shewana3_0440 at 515.516 kb on + strand, within Shewana3_0440 at 515.524 kb on - strand, within Shewana3_0440 at 515.524 kb on - strand, within Shewana3_0440 at 515.593 kb on + strand at 515.593 kb on + strand at 515.596 kb on - strand at 515.601 kb on - strand at 515.603 kb on + strand at 515.736 kb on - strand at 515.956 kb on - strand, within purH at 515.983 kb on - strand, within purH at 515.983 kb on - strand, within purH at 515.983 kb on - strand, within purH at 516.165 kb on - strand, within purH at 516.169 kb on + strand, within purH at 516.169 kb on + strand, within purH at 516.169 kb on + strand, within purH at 516.178 kb on - strand, within purH at 516.258 kb on - strand, within purH at 516.388 kb on - strand, within purH at 516.481 kb on + strand, within purH at 516.481 kb on + strand, within purH at 516.491 kb on + strand, within purH at 516.499 kb on + strand, within purH at 516.499 kb on - strand, within purH at 516.499 kb on - strand, within purH at 516.499 kb on - strand, within purH at 516.556 kb on - strand, within purH at 516.566 kb on - strand, within purH at 516.599 kb on + strand, within purH at 516.774 kb on - strand, within purH at 516.782 kb on - strand, within purH at 517.056 kb on + strand, within purH at 517.064 kb on + strand, within purH at 517.154 kb on + strand, within purH at 517.176 kb on - strand, within purH at 517.226 kb on + strand at 517.385 kb on + strand at 517.566 kb on + strand at 517.640 kb on + strand at 517.648 kb on - strand at 517.679 kb on + strand at 517.713 kb on + strand at 517.714 kb on + strand at 517.779 kb on - strand, within zntR at 517.807 kb on - strand, within zntR at 517.835 kb on + strand, within zntR at 517.843 kb on - strand, within zntR at 517.952 kb on + strand, within zntR at 518.115 kb on - strand, within zntR at 518.147 kb on - strand, within zntR at 518.245 kb on + strand at 518.375 kb on - strand, within Shewana3_0443
Per-strain Table
Position Strand Gene LocusTag Fraction Carbon source Sucrose 5 mM remove 514,845 + Shewana3_0440 0.36 -4.5 514,853 - Shewana3_0440 0.36 -3.0 514,919 + Shewana3_0440 0.41 -3.7 514,999 - Shewana3_0440 0.47 -2.6 515,103 - Shewana3_0440 0.55 -4.3 515,209 + Shewana3_0440 0.64 -1.6 515,283 + Shewana3_0440 0.69 -3.0 515,291 - Shewana3_0440 0.70 -3.1 515,318 - Shewana3_0440 0.72 -1.6 515,352 - Shewana3_0440 0.75 -3.3 515,366 + Shewana3_0440 0.76 -3.6 515,374 - Shewana3_0440 0.76 -2.5 515,374 - Shewana3_0440 0.76 -4.3 515,425 + Shewana3_0440 0.80 -2.9 515,433 - Shewana3_0440 0.81 -3.3 515,462 + Shewana3_0440 0.83 -2.7 515,516 + Shewana3_0440 0.87 -3.7 515,516 + Shewana3_0440 0.87 -3.6 515,524 - Shewana3_0440 0.88 +0.5 515,524 - Shewana3_0440 0.88 -1.8 515,593 + -3.0 515,593 + -3.6 515,596 - -2.6 515,601 - -2.6 515,603 + -3.8 515,736 - -0.1 515,956 - purH Shewana3_0441 0.11 -4.5 515,983 - purH Shewana3_0441 0.12 -3.5 515,983 - purH Shewana3_0441 0.12 -3.6 515,983 - purH Shewana3_0441 0.12 -3.6 516,165 - purH Shewana3_0441 0.24 -4.1 516,169 + purH Shewana3_0441 0.24 -4.2 516,169 + purH Shewana3_0441 0.24 -4.6 516,169 + purH Shewana3_0441 0.24 -4.6 516,178 - purH Shewana3_0441 0.24 -3.9 516,258 - purH Shewana3_0441 0.29 -3.2 516,388 - purH Shewana3_0441 0.38 -2.5 516,481 + purH Shewana3_0441 0.43 -2.7 516,481 + purH Shewana3_0441 0.43 -4.0 516,491 + purH Shewana3_0441 0.44 -3.2 516,499 + purH Shewana3_0441 0.45 -4.8 516,499 - purH Shewana3_0441 0.45 -3.1 516,499 - purH Shewana3_0441 0.45 -4.5 516,499 - purH Shewana3_0441 0.45 -3.0 516,556 - purH Shewana3_0441 0.48 -4.2 516,566 - purH Shewana3_0441 0.49 -4.5 516,599 + purH Shewana3_0441 0.51 -2.6 516,774 - purH Shewana3_0441 0.62 -4.0 516,782 - purH Shewana3_0441 0.62 -3.9 517,056 + purH Shewana3_0441 0.79 -2.3 517,064 + purH Shewana3_0441 0.80 -4.7 517,154 + purH Shewana3_0441 0.85 -3.0 517,176 - purH Shewana3_0441 0.87 -3.6 517,226 + -3.4 517,385 + -3.2 517,566 + +0.6 517,640 + +0.1 517,648 - -0.1 517,679 + -0.0 517,713 + +0.9 517,714 + +0.6 517,779 - zntR Shewana3_0442 0.12 -0.5 517,807 - zntR Shewana3_0442 0.18 +0.5 517,835 + zntR Shewana3_0442 0.24 +0.1 517,843 - zntR Shewana3_0442 0.26 -0.3 517,952 + zntR Shewana3_0442 0.49 -0.3 518,115 - zntR Shewana3_0442 0.83 -0.4 518,147 - zntR Shewana3_0442 0.90 -0.1 518,245 + +0.7 518,375 - Shewana3_0443 0.12 -0.0
Or see this region's nucleotide sequence