Experiment: Carbon source Sucrose 5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_0375 and Shewana3_R0020 are separated by 208 nucleotides Shewana3_R0020 and Shewana3_R0021 are separated by 53 nucleotides Shewana3_R0021 and Shewana3_R0022 are separated by 14 nucleotides Shewana3_R0022 and Shewana3_R0023 are separated by 53 nucleotides Shewana3_R0023 and Shewana3_0376 are separated by 189 nucleotides Shewana3_0376 and Shewana3_0377 are separated by 8 nucleotides Shewana3_0377 and Shewana3_0378 are separated by 18 nucleotides
Shewana3_0375: Shewana3_0375 - diguanylate cyclase/phosphodiesterase with GAF sensor (RefSeq), at 413,613 to 416,243
_0375
Shewana3_R0020: Shewana3_R0020 - tRNA-Arg (RefSeq), at 416,452 to 416,528
_R0020
Shewana3_R0021: Shewana3_R0021 - tRNA-His (RefSeq), at 416,582 to 416,657
_R0021
Shewana3_R0022: Shewana3_R0022 - tRNA-Pro (RefSeq), at 416,672 to 416,748
_R0022
Shewana3_R0023: Shewana3_R0023 - tRNA-Pro (RefSeq), at 416,802 to 416,878
_R0023
Shewana3_0376: Shewana3_0376 - diguanylate cyclase/phosphodiesterase (RefSeq), at 417,068 to 418,987
_0376
Shewana3_0377: Shewana3_0377 - hypothetical protein (RefSeq), at 418,996 to 419,703
_0377
Shewana3_0378: Shewana3_0378 - OmpA/MotB domain-containing protein (RefSeq), at 419,722 to 420,330
_0378
Position (kb) 417
418
419 Strain fitness (log2 ratio) -1
0
1 at 416.148 kb on - strand at 417.114 kb on + strand at 417.461 kb on + strand, within Shewana3_0376 at 417.463 kb on + strand, within Shewana3_0376 at 417.505 kb on - strand, within Shewana3_0376 at 417.597 kb on + strand, within Shewana3_0376 at 417.618 kb on + strand, within Shewana3_0376 at 417.618 kb on + strand, within Shewana3_0376 at 417.618 kb on + strand, within Shewana3_0376 at 417.830 kb on - strand, within Shewana3_0376 at 417.974 kb on + strand, within Shewana3_0376 at 418.143 kb on + strand, within Shewana3_0376 at 418.213 kb on + strand, within Shewana3_0376 at 418.226 kb on - strand, within Shewana3_0376 at 418.244 kb on - strand, within Shewana3_0376 at 418.256 kb on + strand, within Shewana3_0376 at 418.263 kb on - strand, within Shewana3_0376 at 418.344 kb on - strand, within Shewana3_0376 at 418.456 kb on - strand, within Shewana3_0376 at 418.472 kb on + strand, within Shewana3_0376 at 418.644 kb on + strand, within Shewana3_0376 at 418.644 kb on + strand, within Shewana3_0376 at 418.711 kb on - strand, within Shewana3_0376 at 418.859 kb on + strand at 418.897 kb on + strand at 418.979 kb on - strand at 418.996 kb on - strand at 419.047 kb on - strand at 419.077 kb on + strand, within Shewana3_0377 at 419.190 kb on + strand, within Shewana3_0377 at 419.195 kb on + strand, within Shewana3_0377 at 419.199 kb on - strand, within Shewana3_0377 at 419.219 kb on + strand, within Shewana3_0377 at 419.273 kb on + strand, within Shewana3_0377 at 419.318 kb on - strand, within Shewana3_0377 at 419.558 kb on - strand, within Shewana3_0377 at 419.560 kb on - strand, within Shewana3_0377 at 419.685 kb on + strand at 419.763 kb on + strand at 419.888 kb on - strand, within Shewana3_0378
Per-strain Table
Position Strand Gene LocusTag Fraction Carbon source Sucrose 5 mM remove 416,148 - +0.6 417,114 + -1.1 417,461 + Shewana3_0376 0.20 -0.3 417,463 + Shewana3_0376 0.21 +0.0 417,505 - Shewana3_0376 0.23 +0.4 417,597 + Shewana3_0376 0.28 +0.5 417,618 + Shewana3_0376 0.29 -1.4 417,618 + Shewana3_0376 0.29 -0.8 417,618 + Shewana3_0376 0.29 +0.1 417,830 - Shewana3_0376 0.40 -0.2 417,974 + Shewana3_0376 0.47 +0.3 418,143 + Shewana3_0376 0.56 +0.1 418,213 + Shewana3_0376 0.60 -0.4 418,226 - Shewana3_0376 0.60 +0.4 418,244 - Shewana3_0376 0.61 -0.2 418,256 + Shewana3_0376 0.62 +1.6 418,263 - Shewana3_0376 0.62 -0.6 418,344 - Shewana3_0376 0.66 -0.2 418,456 - Shewana3_0376 0.72 -0.3 418,472 + Shewana3_0376 0.73 -0.0 418,644 + Shewana3_0376 0.82 -0.9 418,644 + Shewana3_0376 0.82 -0.4 418,711 - Shewana3_0376 0.86 -0.3 418,859 + -0.1 418,897 + +0.9 418,979 - -0.1 418,996 - -0.1 419,047 - -0.9 419,077 + Shewana3_0377 0.11 +0.3 419,190 + Shewana3_0377 0.27 -0.1 419,195 + Shewana3_0377 0.28 +0.3 419,199 - Shewana3_0377 0.29 +0.3 419,219 + Shewana3_0377 0.31 +0.2 419,273 + Shewana3_0377 0.39 -0.8 419,318 - Shewana3_0377 0.45 -1.5 419,558 - Shewana3_0377 0.79 +0.2 419,560 - Shewana3_0377 0.80 +0.3 419,685 + +0.9 419,763 + +0.5 419,888 - Shewana3_0378 0.27 +1.0
Or see this region's nucleotide sequence