Experiment: Carbon source Sucrose 5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_0022 and fadA are separated by 224 nucleotides fadA and fadB are separated by 22 nucleotides
Shewana3_0022: Shewana3_0022 - diguanylate cyclase (RefSeq), at 26,815 to 28,026
_0022
Shewana3_0023: fadA - Acetyl-CoA C-acyltransferase (EC 2.3.1.16) <small>(from data)</small>, at 28,251 to 29,414
fadA
Shewana3_0024: fadB - Enoyl-CoA hydratase (EC 4.2.1.17); 3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.35) <small>(from data)</small>, at 29,437 to 31,587
fadB
Position (kb) 28
29
30 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 27.279 kb on - strand, within Shewana3_0022 at 27.314 kb on - strand, within Shewana3_0022 at 27.315 kb on + strand, within Shewana3_0022 at 27.340 kb on + strand, within Shewana3_0022 at 27.340 kb on + strand, within Shewana3_0022 at 27.340 kb on + strand, within Shewana3_0022 at 27.348 kb on - strand, within Shewana3_0022 at 27.381 kb on + strand, within Shewana3_0022 at 27.438 kb on + strand, within Shewana3_0022 at 27.446 kb on - strand, within Shewana3_0022 at 27.763 kb on - strand, within Shewana3_0022 at 27.763 kb on - strand, within Shewana3_0022 at 27.763 kb on - strand, within Shewana3_0022 at 27.763 kb on - strand, within Shewana3_0022 at 27.831 kb on + strand, within Shewana3_0022 at 27.850 kb on + strand, within Shewana3_0022 at 27.978 kb on + strand at 28.041 kb on - strand at 28.069 kb on + strand at 28.069 kb on + strand at 28.069 kb on + strand at 28.071 kb on + strand at 28.077 kb on - strand at 28.077 kb on - strand at 28.077 kb on - strand at 28.143 kb on - strand at 28.169 kb on + strand at 28.249 kb on + strand at 28.256 kb on - strand at 28.283 kb on + strand at 28.283 kb on + strand at 28.292 kb on - strand at 28.375 kb on - strand, within fadA at 28.377 kb on + strand, within fadA at 28.385 kb on - strand, within fadA at 28.418 kb on - strand, within fadA at 28.428 kb on - strand, within fadA at 28.438 kb on - strand, within fadA at 28.445 kb on + strand, within fadA at 28.445 kb on + strand, within fadA at 28.454 kb on + strand, within fadA at 28.464 kb on + strand, within fadA at 28.473 kb on - strand, within fadA at 28.473 kb on - strand, within fadA at 28.473 kb on - strand, within fadA at 28.734 kb on + strand, within fadA at 28.742 kb on - strand, within fadA at 28.804 kb on + strand, within fadA at 28.841 kb on - strand, within fadA at 28.874 kb on - strand, within fadA at 28.903 kb on - strand, within fadA at 28.931 kb on + strand, within fadA at 28.947 kb on - strand, within fadA at 29.012 kb on - strand, within fadA at 29.042 kb on - strand, within fadA at 29.065 kb on - strand, within fadA at 29.220 kb on - strand, within fadA at 29.222 kb on - strand, within fadA at 29.225 kb on + strand, within fadA at 29.233 kb on - strand, within fadA at 29.233 kb on - strand, within fadA at 29.289 kb on - strand, within fadA at 29.297 kb on + strand, within fadA at 29.297 kb on + strand, within fadA at 29.305 kb on - strand at 29.370 kb on + strand at 29.370 kb on + strand at 29.378 kb on - strand at 29.378 kb on - strand at 29.378 kb on - strand at 29.458 kb on - strand at 29.512 kb on - strand at 29.560 kb on - strand at 29.580 kb on - strand at 29.614 kb on - strand at 29.655 kb on + strand, within fadB at 29.663 kb on - strand, within fadB at 29.839 kb on - strand, within fadB at 29.976 kb on - strand, within fadB at 30.174 kb on + strand, within fadB at 30.174 kb on + strand, within fadB at 30.179 kb on + strand, within fadB at 30.182 kb on - strand, within fadB at 30.200 kb on + strand, within fadB at 30.227 kb on + strand, within fadB at 30.227 kb on + strand, within fadB at 30.235 kb on - strand, within fadB at 30.235 kb on - strand, within fadB at 30.235 kb on - strand, within fadB at 30.235 kb on - strand, within fadB
Per-strain Table
Position Strand Gene LocusTag Fraction Carbon source Sucrose 5 mM remove 27,279 - Shewana3_0022 0.38 -3.5 27,314 - Shewana3_0022 0.41 -0.2 27,315 + Shewana3_0022 0.41 -0.6 27,340 + Shewana3_0022 0.43 -0.3 27,340 + Shewana3_0022 0.43 -0.2 27,340 + Shewana3_0022 0.43 +0.3 27,348 - Shewana3_0022 0.44 -0.6 27,381 + Shewana3_0022 0.47 -0.3 27,438 + Shewana3_0022 0.51 -0.6 27,446 - Shewana3_0022 0.52 -0.4 27,763 - Shewana3_0022 0.78 +0.4 27,763 - Shewana3_0022 0.78 +0.2 27,763 - Shewana3_0022 0.78 +0.7 27,763 - Shewana3_0022 0.78 +0.9 27,831 + Shewana3_0022 0.84 +0.9 27,850 + Shewana3_0022 0.85 -1.0 27,978 + +0.7 28,041 - -1.0 28,069 + -0.4 28,069 + +1.2 28,069 + -1.2 28,071 + +0.3 28,077 - -0.0 28,077 - -0.2 28,077 - +0.0 28,143 - -0.4 28,169 + -0.6 28,249 + +0.5 28,256 - -0.8 28,283 + +0.1 28,283 + -1.1 28,292 - -0.8 28,375 - fadA Shewana3_0023 0.11 -0.3 28,377 + fadA Shewana3_0023 0.11 -0.6 28,385 - fadA Shewana3_0023 0.12 -0.4 28,418 - fadA Shewana3_0023 0.14 +0.2 28,428 - fadA Shewana3_0023 0.15 -0.1 28,438 - fadA Shewana3_0023 0.16 -0.8 28,445 + fadA Shewana3_0023 0.17 +0.8 28,445 + fadA Shewana3_0023 0.17 -0.0 28,454 + fadA Shewana3_0023 0.17 -0.2 28,464 + fadA Shewana3_0023 0.18 -0.1 28,473 - fadA Shewana3_0023 0.19 +0.3 28,473 - fadA Shewana3_0023 0.19 -0.4 28,473 - fadA Shewana3_0023 0.19 +0.5 28,734 + fadA Shewana3_0023 0.41 -0.1 28,742 - fadA Shewana3_0023 0.42 +0.1 28,804 + fadA Shewana3_0023 0.48 -0.6 28,841 - fadA Shewana3_0023 0.51 -0.3 28,874 - fadA Shewana3_0023 0.54 -0.9 28,903 - fadA Shewana3_0023 0.56 -0.7 28,931 + fadA Shewana3_0023 0.58 -0.8 28,947 - fadA Shewana3_0023 0.60 -0.7 29,012 - fadA Shewana3_0023 0.65 -0.3 29,042 - fadA Shewana3_0023 0.68 -0.1 29,065 - fadA Shewana3_0023 0.70 -0.2 29,220 - fadA Shewana3_0023 0.83 -1.2 29,222 - fadA Shewana3_0023 0.83 -0.6 29,225 + fadA Shewana3_0023 0.84 -1.0 29,233 - fadA Shewana3_0023 0.84 -0.5 29,233 - fadA Shewana3_0023 0.84 -0.1 29,289 - fadA Shewana3_0023 0.89 +0.5 29,297 + fadA Shewana3_0023 0.90 +0.4 29,297 + fadA Shewana3_0023 0.90 -0.3 29,305 - -0.3 29,370 + -0.9 29,370 + +0.6 29,378 - +0.1 29,378 - +0.4 29,378 - +0.0 29,458 - -0.2 29,512 - -0.8 29,560 - -0.0 29,580 - -0.6 29,614 - -0.4 29,655 + fadB Shewana3_0024 0.10 -0.8 29,663 - fadB Shewana3_0024 0.11 -1.1 29,839 - fadB Shewana3_0024 0.19 -1.0 29,976 - fadB Shewana3_0024 0.25 -0.9 30,174 + fadB Shewana3_0024 0.34 -0.3 30,174 + fadB Shewana3_0024 0.34 -1.4 30,179 + fadB Shewana3_0024 0.34 -0.4 30,182 - fadB Shewana3_0024 0.35 +0.5 30,200 + fadB Shewana3_0024 0.35 -0.3 30,227 + fadB Shewana3_0024 0.37 -0.7 30,227 + fadB Shewana3_0024 0.37 -0.6 30,235 - fadB Shewana3_0024 0.37 -0.5 30,235 - fadB Shewana3_0024 0.37 -0.3 30,235 - fadB Shewana3_0024 0.37 -0.7 30,235 - fadB Shewana3_0024 0.37 +0.4
Or see this region's nucleotide sequence