Experiment: Ammonium chloride (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT0312 and BT0313 are separated by 24 nucleotides BT0313 and BT0314 are separated by 250 nucleotides BT0314 and BT0315 are separated by 205 nucleotides
BT0312: BT0312 - 2-oxoisovalerate dehydrogenase beta subunit (NCBI ptt file), at 375,030 to 377,066
BT0312
BT0313: BT0313 - flavodoxin (NCBI ptt file), at 377,091 to 377,606
BT0313
BT0314: BT0314 - ribosomal protein L11 methyltransferase (NCBI ptt file), at 377,857 to 378,699
BT0314
BT0315: BT0315 - hypothetical protein (NCBI ptt file), at 378,905 to 380,194
BT0315
Position (kb) 377
378
379 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 376.967 kb on + strand at 376.973 kb on + strand at 376.974 kb on - strand at 377.083 kb on + strand at 377.083 kb on + strand at 377.085 kb on + strand at 377.088 kb on + strand at 377.090 kb on + strand at 377.090 kb on + strand at 377.091 kb on - strand at 377.112 kb on + strand at 377.242 kb on + strand, within BT0313 at 377.243 kb on - strand, within BT0313 at 377.280 kb on - strand, within BT0313 at 377.280 kb on - strand, within BT0313 at 377.296 kb on + strand, within BT0313 at 377.578 kb on + strand at 377.612 kb on + strand at 377.646 kb on - strand at 377.648 kb on + strand at 377.650 kb on + strand at 377.651 kb on - strand at 377.728 kb on - strand at 377.778 kb on + strand at 377.779 kb on - strand at 377.785 kb on - strand at 377.788 kb on - strand at 377.793 kb on + strand at 377.793 kb on + strand at 377.796 kb on - strand at 377.796 kb on - strand at 377.859 kb on - strand at 377.899 kb on - strand at 377.905 kb on + strand at 377.964 kb on - strand, within BT0314 at 378.048 kb on + strand, within BT0314 at 378.124 kb on + strand, within BT0314 at 378.124 kb on + strand, within BT0314 at 378.363 kb on - strand, within BT0314 at 378.485 kb on + strand, within BT0314 at 378.490 kb on - strand, within BT0314 at 378.569 kb on - strand, within BT0314 at 378.572 kb on + strand, within BT0314 at 378.573 kb on - strand, within BT0314 at 378.625 kb on + strand at 378.649 kb on + strand at 378.650 kb on - strand at 378.700 kb on - strand at 378.700 kb on - strand at 378.734 kb on + strand at 378.825 kb on + strand at 378.850 kb on + strand at 379.019 kb on + strand at 379.140 kb on + strand, within BT0315 at 379.142 kb on + strand, within BT0315 at 379.217 kb on + strand, within BT0315 at 379.301 kb on + strand, within BT0315
Per-strain Table
Position Strand Gene LocusTag Fraction Ammonium chloride (N) remove 376,967 + -2.6 376,973 + -0.8 376,974 - -0.4 377,083 + +0.4 377,083 + -2.3 377,085 + -1.4 377,088 + -0.6 377,090 + -1.1 377,090 + -0.8 377,091 - -0.5 377,112 + -1.0 377,242 + BT0313 0.29 +1.6 377,243 - BT0313 0.29 -0.4 377,280 - BT0313 0.37 +0.3 377,280 - BT0313 0.37 +1.0 377,296 + BT0313 0.40 -1.2 377,578 + -0.7 377,612 + -3.7 377,646 - +0.6 377,648 + -2.0 377,650 + -0.2 377,651 - -0.7 377,728 - +0.6 377,778 + +0.6 377,779 - +0.2 377,785 - -1.0 377,788 - +0.0 377,793 + -2.4 377,793 + +0.6 377,796 - -2.2 377,796 - -0.4 377,859 - -0.4 377,899 - -2.9 377,905 + -0.2 377,964 - BT0314 0.13 -0.4 378,048 + BT0314 0.23 -0.1 378,124 + BT0314 0.32 +1.6 378,124 + BT0314 0.32 -1.6 378,363 - BT0314 0.60 -2.2 378,485 + BT0314 0.74 -2.4 378,490 - BT0314 0.75 -2.0 378,569 - BT0314 0.84 -2.1 378,572 + BT0314 0.85 -0.4 378,573 - BT0314 0.85 +0.0 378,625 + -1.7 378,649 + -1.4 378,650 - -1.6 378,700 - +0.2 378,700 - +1.0 378,734 + -2.0 378,825 + +0.6 378,850 + +0.9 379,019 + +0.0 379,140 + BT0315 0.18 -0.4 379,142 + BT0315 0.18 -0.7 379,217 + BT0315 0.24 +0.9 379,301 + BT0315 0.31 +0.3
Or see this region's nucleotide sequence