Experiment: R2A with Lomefloxacin 0.001 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS34955 and RR42_RS34960 are separated by 360 nucleotides RR42_RS34960 and RR42_RS34965 are separated by 258 nucleotides
RR42_RS34955: RR42_RS34955 - cytochrome B558 subunit A, at 3,292,893 to 3,294,071
_RS34955
RR42_RS34960: RR42_RS34960 - membrane protein, at 3,294,432 to 3,294,935
_RS34960
RR42_RS34965: RR42_RS34965 - dioxygenase, at 3,295,194 to 3,296,729
_RS34965
Position (kb)
3294
3295 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3293.495 kb on - strand, within RR42_RS34955 at 3293.495 kb on - strand, within RR42_RS34955 at 3293.752 kb on + strand, within RR42_RS34955 at 3293.753 kb on - strand, within RR42_RS34955 at 3293.761 kb on + strand, within RR42_RS34955 at 3293.852 kb on - strand, within RR42_RS34955 at 3293.896 kb on + strand, within RR42_RS34955 at 3293.896 kb on + strand, within RR42_RS34955 at 3293.897 kb on - strand, within RR42_RS34955 at 3293.897 kb on - strand, within RR42_RS34955 at 3293.897 kb on - strand, within RR42_RS34955 at 3293.897 kb on - strand, within RR42_RS34955 at 3294.063 kb on + strand at 3294.072 kb on - strand at 3294.125 kb on + strand at 3294.131 kb on + strand at 3294.131 kb on - strand at 3294.307 kb on - strand at 3294.307 kb on - strand at 3294.441 kb on + strand at 3294.441 kb on + strand at 3294.441 kb on + strand at 3294.441 kb on + strand at 3294.442 kb on - strand at 3294.442 kb on - strand at 3294.512 kb on + strand, within RR42_RS34960 at 3294.513 kb on - strand, within RR42_RS34960 at 3294.513 kb on - strand, within RR42_RS34960 at 3294.563 kb on - strand, within RR42_RS34960 at 3294.563 kb on - strand, within RR42_RS34960 at 3294.907 kb on + strand at 3294.936 kb on - strand at 3294.950 kb on - strand at 3295.014 kb on + strand at 3295.015 kb on - strand at 3295.015 kb on - strand at 3295.052 kb on - strand at 3295.064 kb on + strand at 3295.064 kb on + strand at 3295.064 kb on + strand at 3295.065 kb on - strand at 3295.065 kb on - strand at 3295.065 kb on - strand at 3295.065 kb on - strand at 3295.105 kb on + strand at 3295.105 kb on + strand at 3295.172 kb on + strand at 3295.172 kb on + strand at 3295.427 kb on + strand, within RR42_RS34965 at 3295.595 kb on + strand, within RR42_RS34965 at 3295.596 kb on - strand, within RR42_RS34965 at 3295.596 kb on - strand, within RR42_RS34965
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Lomefloxacin 0.001 mM remove 3,293,495 - RR42_RS34955 0.51 +1.5 3,293,495 - RR42_RS34955 0.51 -1.5 3,293,752 + RR42_RS34955 0.73 -0.6 3,293,753 - RR42_RS34955 0.73 +1.0 3,293,761 + RR42_RS34955 0.74 -0.7 3,293,852 - RR42_RS34955 0.81 -1.1 3,293,896 + RR42_RS34955 0.85 -0.4 3,293,896 + RR42_RS34955 0.85 -0.9 3,293,897 - RR42_RS34955 0.85 +0.7 3,293,897 - RR42_RS34955 0.85 -0.1 3,293,897 - RR42_RS34955 0.85 +0.2 3,293,897 - RR42_RS34955 0.85 -2.8 3,294,063 + +1.0 3,294,072 - +0.1 3,294,125 + -1.2 3,294,131 + +1.2 3,294,131 - +2.1 3,294,307 - +1.5 3,294,307 - +0.6 3,294,441 + -0.7 3,294,441 + +2.6 3,294,441 + +1.3 3,294,441 + -0.5 3,294,442 - +0.3 3,294,442 - -0.0 3,294,512 + RR42_RS34960 0.16 +0.2 3,294,513 - RR42_RS34960 0.16 +1.0 3,294,513 - RR42_RS34960 0.16 +0.7 3,294,563 - RR42_RS34960 0.26 +1.4 3,294,563 - RR42_RS34960 0.26 -0.2 3,294,907 + +1.0 3,294,936 - -1.5 3,294,950 - -0.2 3,295,014 + -1.4 3,295,015 - -2.2 3,295,015 - -2.4 3,295,052 - -1.4 3,295,064 + -1.9 3,295,064 + -2.1 3,295,064 + +0.5 3,295,065 - -0.7 3,295,065 - +0.1 3,295,065 - -0.8 3,295,065 - -1.4 3,295,105 + -0.5 3,295,105 + +1.0 3,295,172 + +2.3 3,295,172 + +0.5 3,295,427 + RR42_RS34965 0.15 -0.2 3,295,595 + RR42_RS34965 0.26 +0.6 3,295,596 - RR42_RS34965 0.26 -0.2 3,295,596 - RR42_RS34965 0.26 -2.5
Or see this region's nucleotide sequence