Experiment: R2A with Lomefloxacin 0.001 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS33775 and RR42_RS33780 are separated by 232 nucleotides RR42_RS33780 and RR42_RS33785 are separated by 288 nucleotides RR42_RS33785 and RR42_RS33790 are separated by 37 nucleotides
RR42_RS33775: RR42_RS33775 - sulfoacetaldehyde acetyltransferase, at 3,006,090 to 3,007,922
_RS33775
RR42_RS33780: RR42_RS33780 - GABA permease, at 3,008,155 to 3,009,579
_RS33780
RR42_RS33785: RR42_RS33785 - transcriptional regulator, at 3,009,868 to 3,010,278
_RS33785
RR42_RS33790: RR42_RS33790 - FAD-dependent oxidoreductase, at 3,010,316 to 3,011,755
_RS33790
Position (kb)
3008
3009
3010 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 3007.905 kb on + strand at 3007.905 kb on + strand at 3007.914 kb on + strand at 3008.098 kb on - strand at 3008.103 kb on + strand at 3008.103 kb on + strand at 3008.103 kb on + strand at 3008.103 kb on + strand at 3008.104 kb on - strand at 3008.104 kb on - strand at 3008.106 kb on + strand at 3008.106 kb on + strand at 3008.106 kb on + strand at 3008.107 kb on - strand at 3008.108 kb on + strand at 3008.108 kb on + strand at 3008.109 kb on - strand at 3008.109 kb on - strand at 3008.109 kb on - strand at 3008.109 kb on - strand at 3008.110 kb on + strand at 3008.110 kb on + strand at 3008.111 kb on - strand at 3008.111 kb on - strand at 3008.398 kb on + strand, within RR42_RS33780 at 3008.398 kb on + strand, within RR42_RS33780 at 3008.473 kb on + strand, within RR42_RS33780 at 3008.474 kb on - strand, within RR42_RS33780 at 3008.474 kb on - strand, within RR42_RS33780 at 3008.474 kb on - strand, within RR42_RS33780 at 3008.479 kb on + strand, within RR42_RS33780 at 3008.479 kb on + strand, within RR42_RS33780 at 3008.480 kb on - strand, within RR42_RS33780 at 3008.480 kb on - strand, within RR42_RS33780 at 3008.480 kb on - strand, within RR42_RS33780 at 3008.576 kb on + strand, within RR42_RS33780 at 3008.577 kb on - strand, within RR42_RS33780 at 3008.577 kb on - strand, within RR42_RS33780 at 3008.577 kb on - strand, within RR42_RS33780 at 3008.770 kb on - strand, within RR42_RS33780 at 3008.818 kb on + strand, within RR42_RS33780 at 3008.819 kb on - strand, within RR42_RS33780 at 3008.923 kb on + strand, within RR42_RS33780 at 3008.923 kb on + strand, within RR42_RS33780 at 3008.923 kb on + strand, within RR42_RS33780 at 3008.923 kb on + strand, within RR42_RS33780 at 3008.923 kb on + strand, within RR42_RS33780 at 3008.924 kb on - strand, within RR42_RS33780 at 3008.924 kb on - strand, within RR42_RS33780 at 3008.924 kb on - strand, within RR42_RS33780 at 3008.924 kb on - strand, within RR42_RS33780 at 3008.990 kb on - strand, within RR42_RS33780 at 3008.990 kb on - strand, within RR42_RS33780 at 3008.990 kb on - strand, within RR42_RS33780 at 3008.990 kb on - strand, within RR42_RS33780 at 3009.088 kb on + strand, within RR42_RS33780 at 3009.230 kb on - strand, within RR42_RS33780 at 3009.230 kb on - strand, within RR42_RS33780 at 3009.234 kb on - strand, within RR42_RS33780 at 3009.271 kb on + strand, within RR42_RS33780 at 3009.271 kb on + strand, within RR42_RS33780 at 3009.272 kb on - strand, within RR42_RS33780 at 3009.295 kb on + strand, within RR42_RS33780 at 3009.295 kb on + strand, within RR42_RS33780 at 3009.296 kb on - strand, within RR42_RS33780 at 3009.445 kb on - strand at 3009.508 kb on + strand at 3009.509 kb on - strand at 3009.763 kb on - strand at 3009.785 kb on + strand at 3009.785 kb on + strand at 3009.785 kb on + strand at 3009.786 kb on - strand at 3009.799 kb on - strand at 3010.433 kb on + strand at 3010.434 kb on - strand at 3010.445 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Lomefloxacin 0.001 mM remove 3,007,905 + -0.2 3,007,905 + -0.2 3,007,914 + -1.5 3,008,098 - -1.0 3,008,103 + +0.3 3,008,103 + -1.1 3,008,103 + -1.5 3,008,103 + -0.6 3,008,104 - -0.4 3,008,104 - +0.5 3,008,106 + +1.1 3,008,106 + -0.2 3,008,106 + -2.3 3,008,107 - +0.3 3,008,108 + +0.5 3,008,108 + -0.4 3,008,109 - +1.5 3,008,109 - -1.4 3,008,109 - +0.3 3,008,109 - +0.3 3,008,110 + -0.4 3,008,110 + +0.5 3,008,111 - +1.8 3,008,111 - -0.4 3,008,398 + RR42_RS33780 0.17 -0.3 3,008,398 + RR42_RS33780 0.17 +0.3 3,008,473 + RR42_RS33780 0.22 -1.5 3,008,474 - RR42_RS33780 0.22 +0.4 3,008,474 - RR42_RS33780 0.22 +0.4 3,008,474 - RR42_RS33780 0.22 +2.3 3,008,479 + RR42_RS33780 0.23 +0.2 3,008,479 + RR42_RS33780 0.23 +0.3 3,008,480 - RR42_RS33780 0.23 +0.2 3,008,480 - RR42_RS33780 0.23 +1.1 3,008,480 - RR42_RS33780 0.23 -0.1 3,008,576 + RR42_RS33780 0.30 -0.9 3,008,577 - RR42_RS33780 0.30 -1.1 3,008,577 - RR42_RS33780 0.30 +1.1 3,008,577 - RR42_RS33780 0.30 -0.5 3,008,770 - RR42_RS33780 0.43 -1.8 3,008,818 + RR42_RS33780 0.47 -2.5 3,008,819 - RR42_RS33780 0.47 +1.4 3,008,923 + RR42_RS33780 0.54 +1.6 3,008,923 + RR42_RS33780 0.54 -0.4 3,008,923 + RR42_RS33780 0.54 -1.3 3,008,923 + RR42_RS33780 0.54 -1.2 3,008,923 + RR42_RS33780 0.54 -0.9 3,008,924 - RR42_RS33780 0.54 +1.4 3,008,924 - RR42_RS33780 0.54 -3.1 3,008,924 - RR42_RS33780 0.54 +0.4 3,008,924 - RR42_RS33780 0.54 -0.9 3,008,990 - RR42_RS33780 0.59 -0.9 3,008,990 - RR42_RS33780 0.59 -1.6 3,008,990 - RR42_RS33780 0.59 -0.0 3,008,990 - RR42_RS33780 0.59 -0.2 3,009,088 + RR42_RS33780 0.65 +0.3 3,009,230 - RR42_RS33780 0.75 -2.7 3,009,230 - RR42_RS33780 0.75 -0.1 3,009,234 - RR42_RS33780 0.76 -1.1 3,009,271 + RR42_RS33780 0.78 +0.6 3,009,271 + RR42_RS33780 0.78 +0.6 3,009,272 - RR42_RS33780 0.78 +1.7 3,009,295 + RR42_RS33780 0.80 +0.4 3,009,295 + RR42_RS33780 0.80 -1.7 3,009,296 - RR42_RS33780 0.80 +0.8 3,009,445 - +0.3 3,009,508 + +3.0 3,009,509 - +0.5 3,009,763 - -1.2 3,009,785 + -1.6 3,009,785 + -0.1 3,009,785 + -0.7 3,009,786 - -1.4 3,009,799 - -1.1 3,010,433 + +0.4 3,010,434 - +1.5 3,010,445 + +1.0
Or see this region's nucleotide sequence