Experiment: R2A with Lomefloxacin 0.001 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS33045 and RR42_RS33050 are separated by 49 nucleotides RR42_RS33050 and RR42_RS33055 are separated by 21 nucleotides
RR42_RS33045: RR42_RS33045 - pyruvate dehydrogenase, at 2,819,850 to 2,820,914
_RS33045
RR42_RS33050: RR42_RS33050 - 2-oxoisovalerate dehydrogenase, at 2,820,964 to 2,821,941
_RS33050
RR42_RS33055: RR42_RS33055 - branched-chain alpha-keto acid dehydrogenase subunit E2, at 2,821,963 to 2,823,066
_RS33055
Position (kb)
2820
2821
2822 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2819.964 kb on + strand, within RR42_RS33045 at 2819.964 kb on + strand, within RR42_RS33045 at 2820.172 kb on + strand, within RR42_RS33045 at 2820.173 kb on - strand, within RR42_RS33045 at 2820.177 kb on + strand, within RR42_RS33045 at 2820.177 kb on + strand, within RR42_RS33045 at 2820.178 kb on - strand, within RR42_RS33045 at 2820.178 kb on - strand, within RR42_RS33045 at 2820.178 kb on - strand, within RR42_RS33045 at 2820.282 kb on + strand, within RR42_RS33045 at 2820.360 kb on + strand, within RR42_RS33045 at 2820.375 kb on - strand, within RR42_RS33045 at 2820.613 kb on - strand, within RR42_RS33045 at 2820.718 kb on - strand, within RR42_RS33045 at 2820.807 kb on + strand, within RR42_RS33045 at 2820.807 kb on + strand, within RR42_RS33045 at 2820.856 kb on - strand at 2820.856 kb on - strand at 2820.856 kb on - strand at 2821.222 kb on - strand, within RR42_RS33050 at 2821.222 kb on - strand, within RR42_RS33050 at 2821.222 kb on - strand, within RR42_RS33050 at 2821.222 kb on - strand, within RR42_RS33050 at 2821.403 kb on + strand, within RR42_RS33050 at 2821.403 kb on + strand, within RR42_RS33050 at 2821.404 kb on - strand, within RR42_RS33050 at 2821.491 kb on - strand, within RR42_RS33050 at 2821.493 kb on - strand, within RR42_RS33050 at 2821.514 kb on + strand, within RR42_RS33050 at 2821.514 kb on + strand, within RR42_RS33050 at 2821.515 kb on - strand, within RR42_RS33050 at 2821.545 kb on + strand, within RR42_RS33050 at 2821.704 kb on - strand, within RR42_RS33050 at 2821.811 kb on - strand, within RR42_RS33050 at 2821.879 kb on + strand at 2821.880 kb on - strand at 2821.880 kb on - strand at 2821.885 kb on + strand at 2821.886 kb on - strand at 2822.103 kb on - strand, within RR42_RS33055 at 2822.626 kb on + strand, within RR42_RS33055 at 2822.627 kb on - strand, within RR42_RS33055 at 2822.863 kb on + strand, within RR42_RS33055 at 2822.863 kb on + strand, within RR42_RS33055 at 2822.863 kb on + strand, within RR42_RS33055 at 2822.864 kb on - strand, within RR42_RS33055 at 2822.864 kb on - strand, within RR42_RS33055 at 2822.864 kb on - strand, within RR42_RS33055 at 2822.864 kb on - strand, within RR42_RS33055
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Lomefloxacin 0.001 mM remove 2,819,964 + RR42_RS33045 0.11 -0.6 2,819,964 + RR42_RS33045 0.11 -1.3 2,820,172 + RR42_RS33045 0.30 -0.3 2,820,173 - RR42_RS33045 0.30 +0.8 2,820,177 + RR42_RS33045 0.31 -0.3 2,820,177 + RR42_RS33045 0.31 -2.1 2,820,178 - RR42_RS33045 0.31 +0.8 2,820,178 - RR42_RS33045 0.31 -3.5 2,820,178 - RR42_RS33045 0.31 +0.7 2,820,282 + RR42_RS33045 0.41 -0.8 2,820,360 + RR42_RS33045 0.48 -0.2 2,820,375 - RR42_RS33045 0.49 -2.6 2,820,613 - RR42_RS33045 0.72 +0.6 2,820,718 - RR42_RS33045 0.82 -0.3 2,820,807 + RR42_RS33045 0.90 -0.7 2,820,807 + RR42_RS33045 0.90 +1.2 2,820,856 - -0.1 2,820,856 - +1.2 2,820,856 - +0.1 2,821,222 - RR42_RS33050 0.26 +0.6 2,821,222 - RR42_RS33050 0.26 -0.1 2,821,222 - RR42_RS33050 0.26 +0.4 2,821,222 - RR42_RS33050 0.26 -1.1 2,821,403 + RR42_RS33050 0.45 -0.7 2,821,403 + RR42_RS33050 0.45 +0.2 2,821,404 - RR42_RS33050 0.45 -0.8 2,821,491 - RR42_RS33050 0.54 -0.5 2,821,493 - RR42_RS33050 0.54 +0.3 2,821,514 + RR42_RS33050 0.56 -0.8 2,821,514 + RR42_RS33050 0.56 -1.2 2,821,515 - RR42_RS33050 0.56 -0.9 2,821,545 + RR42_RS33050 0.59 -3.0 2,821,704 - RR42_RS33050 0.76 -0.4 2,821,811 - RR42_RS33050 0.87 -1.6 2,821,879 + +1.6 2,821,880 - -3.6 2,821,880 - -0.5 2,821,885 + -1.7 2,821,886 - +0.7 2,822,103 - RR42_RS33055 0.13 -1.8 2,822,626 + RR42_RS33055 0.60 +0.5 2,822,627 - RR42_RS33055 0.60 -1.8 2,822,863 + RR42_RS33055 0.82 -0.2 2,822,863 + RR42_RS33055 0.82 +0.1 2,822,863 + RR42_RS33055 0.82 -0.9 2,822,864 - RR42_RS33055 0.82 -0.9 2,822,864 - RR42_RS33055 0.82 -0.4 2,822,864 - RR42_RS33055 0.82 -0.3 2,822,864 - RR42_RS33055 0.82 -2.7
Or see this region's nucleotide sequence