Strain Fitness in Cupriavidus basilensis FW507-4G11 around RR42_RS33050

Experiment: R2A with Lomefloxacin 0.001 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRR42_RS33045 and RR42_RS33050 are separated by 49 nucleotidesRR42_RS33050 and RR42_RS33055 are separated by 21 nucleotides RR42_RS33045: RR42_RS33045 - pyruvate dehydrogenase, at 2,819,850 to 2,820,914 _RS33045 RR42_RS33050: RR42_RS33050 - 2-oxoisovalerate dehydrogenase, at 2,820,964 to 2,821,941 _RS33050 RR42_RS33055: RR42_RS33055 - branched-chain alpha-keto acid dehydrogenase subunit E2, at 2,821,963 to 2,823,066 _RS33055 Position (kb) 2820 2821 2822Strain fitness (log2 ratio) -3 -2 -1 0 1at 2819.964 kb on + strand, within RR42_RS33045at 2819.964 kb on + strand, within RR42_RS33045at 2820.172 kb on + strand, within RR42_RS33045at 2820.173 kb on - strand, within RR42_RS33045at 2820.177 kb on + strand, within RR42_RS33045at 2820.177 kb on + strand, within RR42_RS33045at 2820.178 kb on - strand, within RR42_RS33045at 2820.178 kb on - strand, within RR42_RS33045at 2820.178 kb on - strand, within RR42_RS33045at 2820.282 kb on + strand, within RR42_RS33045at 2820.360 kb on + strand, within RR42_RS33045at 2820.375 kb on - strand, within RR42_RS33045at 2820.613 kb on - strand, within RR42_RS33045at 2820.718 kb on - strand, within RR42_RS33045at 2820.807 kb on + strand, within RR42_RS33045at 2820.807 kb on + strand, within RR42_RS33045at 2820.856 kb on - strandat 2820.856 kb on - strandat 2820.856 kb on - strandat 2821.222 kb on - strand, within RR42_RS33050at 2821.222 kb on - strand, within RR42_RS33050at 2821.222 kb on - strand, within RR42_RS33050at 2821.222 kb on - strand, within RR42_RS33050at 2821.403 kb on + strand, within RR42_RS33050at 2821.403 kb on + strand, within RR42_RS33050at 2821.404 kb on - strand, within RR42_RS33050at 2821.491 kb on - strand, within RR42_RS33050at 2821.493 kb on - strand, within RR42_RS33050at 2821.514 kb on + strand, within RR42_RS33050at 2821.514 kb on + strand, within RR42_RS33050at 2821.515 kb on - strand, within RR42_RS33050at 2821.545 kb on + strand, within RR42_RS33050at 2821.704 kb on - strand, within RR42_RS33050at 2821.811 kb on - strand, within RR42_RS33050at 2821.879 kb on + strandat 2821.880 kb on - strandat 2821.880 kb on - strandat 2821.885 kb on + strandat 2821.886 kb on - strandat 2822.103 kb on - strand, within RR42_RS33055at 2822.626 kb on + strand, within RR42_RS33055at 2822.627 kb on - strand, within RR42_RS33055at 2822.863 kb on + strand, within RR42_RS33055at 2822.863 kb on + strand, within RR42_RS33055at 2822.863 kb on + strand, within RR42_RS33055at 2822.864 kb on - strand, within RR42_RS33055at 2822.864 kb on - strand, within RR42_RS33055at 2822.864 kb on - strand, within RR42_RS33055at 2822.864 kb on - strand, within RR42_RS33055

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Per-strain Table

Position Strand Gene LocusTag Fraction R2A with Lomefloxacin 0.001 mM
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2,819,964 + RR42_RS33045 0.11 -0.6
2,819,964 + RR42_RS33045 0.11 -1.3
2,820,172 + RR42_RS33045 0.30 -0.3
2,820,173 - RR42_RS33045 0.30 +0.8
2,820,177 + RR42_RS33045 0.31 -0.3
2,820,177 + RR42_RS33045 0.31 -2.1
2,820,178 - RR42_RS33045 0.31 +0.8
2,820,178 - RR42_RS33045 0.31 -3.5
2,820,178 - RR42_RS33045 0.31 +0.7
2,820,282 + RR42_RS33045 0.41 -0.8
2,820,360 + RR42_RS33045 0.48 -0.2
2,820,375 - RR42_RS33045 0.49 -2.6
2,820,613 - RR42_RS33045 0.72 +0.6
2,820,718 - RR42_RS33045 0.82 -0.3
2,820,807 + RR42_RS33045 0.90 -0.7
2,820,807 + RR42_RS33045 0.90 +1.2
2,820,856 - -0.1
2,820,856 - +1.2
2,820,856 - +0.1
2,821,222 - RR42_RS33050 0.26 +0.6
2,821,222 - RR42_RS33050 0.26 -0.1
2,821,222 - RR42_RS33050 0.26 +0.4
2,821,222 - RR42_RS33050 0.26 -1.1
2,821,403 + RR42_RS33050 0.45 -0.7
2,821,403 + RR42_RS33050 0.45 +0.2
2,821,404 - RR42_RS33050 0.45 -0.8
2,821,491 - RR42_RS33050 0.54 -0.5
2,821,493 - RR42_RS33050 0.54 +0.3
2,821,514 + RR42_RS33050 0.56 -0.8
2,821,514 + RR42_RS33050 0.56 -1.2
2,821,515 - RR42_RS33050 0.56 -0.9
2,821,545 + RR42_RS33050 0.59 -3.0
2,821,704 - RR42_RS33050 0.76 -0.4
2,821,811 - RR42_RS33050 0.87 -1.6
2,821,879 + +1.6
2,821,880 - -3.6
2,821,880 - -0.5
2,821,885 + -1.7
2,821,886 - +0.7
2,822,103 - RR42_RS33055 0.13 -1.8
2,822,626 + RR42_RS33055 0.60 +0.5
2,822,627 - RR42_RS33055 0.60 -1.8
2,822,863 + RR42_RS33055 0.82 -0.2
2,822,863 + RR42_RS33055 0.82 +0.1
2,822,863 + RR42_RS33055 0.82 -0.9
2,822,864 - RR42_RS33055 0.82 -0.9
2,822,864 - RR42_RS33055 0.82 -0.4
2,822,864 - RR42_RS33055 0.82 -0.3
2,822,864 - RR42_RS33055 0.82 -2.7

Or see this region's nucleotide sequence