Experiment: R2A with Lomefloxacin 0.001 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS32760 and RR42_RS32765 are separated by 78 nucleotides RR42_RS32765 and RR42_RS32770 are separated by 101 nucleotides RR42_RS32770 and RR42_RS32775 are separated by 60 nucleotides RR42_RS32775 and RR42_RS32780 are separated by 270 nucleotides
RR42_RS32760: RR42_RS32760 - hypothetical protein, at 2,745,508 to 2,746,641
_RS32760
RR42_RS32765: RR42_RS32765 - hypothetical protein, at 2,746,720 to 2,747,214
_RS32765
RR42_RS32770: RR42_RS32770 - transglycosylase, at 2,747,316 to 2,747,564
_RS32770
RR42_RS32775: RR42_RS32775 - hypothetical protein, at 2,747,625 to 2,747,807
_RS32775
RR42_RS32780: RR42_RS32780 - multidrug transporter, at 2,748,078 to 2,748,986
_RS32780
Position (kb)
2746
2747
2748 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 2745.802 kb on - strand, within RR42_RS32760 at 2745.916 kb on + strand, within RR42_RS32760 at 2745.917 kb on - strand, within RR42_RS32760 at 2745.973 kb on + strand, within RR42_RS32760 at 2745.975 kb on + strand, within RR42_RS32760 at 2745.975 kb on + strand, within RR42_RS32760 at 2745.975 kb on + strand, within RR42_RS32760 at 2745.975 kb on + strand, within RR42_RS32760 at 2745.975 kb on + strand, within RR42_RS32760 at 2745.979 kb on + strand, within RR42_RS32760 at 2745.979 kb on + strand, within RR42_RS32760 at 2745.979 kb on + strand, within RR42_RS32760 at 2745.979 kb on + strand, within RR42_RS32760 at 2745.980 kb on - strand, within RR42_RS32760 at 2745.983 kb on - strand, within RR42_RS32760 at 2746.021 kb on + strand, within RR42_RS32760 at 2746.022 kb on - strand, within RR42_RS32760 at 2746.022 kb on - strand, within RR42_RS32760 at 2746.051 kb on + strand, within RR42_RS32760 at 2746.052 kb on - strand, within RR42_RS32760 at 2746.141 kb on + strand, within RR42_RS32760 at 2746.142 kb on - strand, within RR42_RS32760 at 2746.142 kb on - strand, within RR42_RS32760 at 2746.142 kb on - strand, within RR42_RS32760 at 2746.381 kb on - strand, within RR42_RS32760 at 2746.809 kb on - strand, within RR42_RS32765 at 2747.059 kb on - strand, within RR42_RS32765 at 2747.059 kb on - strand, within RR42_RS32765 at 2747.059 kb on - strand, within RR42_RS32765 at 2747.267 kb on + strand at 2747.314 kb on + strand at 2747.317 kb on + strand at 2747.317 kb on + strand at 2747.318 kb on - strand at 2747.318 kb on - strand at 2747.341 kb on + strand, within RR42_RS32770 at 2747.407 kb on + strand, within RR42_RS32770 at 2747.505 kb on - strand, within RR42_RS32770 at 2747.663 kb on + strand, within RR42_RS32775 at 2747.663 kb on + strand, within RR42_RS32775 at 2747.664 kb on - strand, within RR42_RS32775 at 2747.664 kb on - strand, within RR42_RS32775 at 2747.796 kb on + strand at 2747.797 kb on - strand at 2747.812 kb on - strand at 2747.812 kb on - strand at 2747.812 kb on - strand at 2747.896 kb on - strand at 2747.899 kb on + strand at 2747.900 kb on - strand at 2747.900 kb on - strand at 2747.929 kb on + strand at 2747.930 kb on - strand at 2747.930 kb on - strand at 2747.992 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Lomefloxacin 0.001 mM remove 2,745,802 - RR42_RS32760 0.26 -0.4 2,745,916 + RR42_RS32760 0.36 +2.1 2,745,917 - RR42_RS32760 0.36 +0.7 2,745,973 + RR42_RS32760 0.41 +0.1 2,745,975 + RR42_RS32760 0.41 -1.0 2,745,975 + RR42_RS32760 0.41 -0.8 2,745,975 + RR42_RS32760 0.41 -1.2 2,745,975 + RR42_RS32760 0.41 -1.3 2,745,975 + RR42_RS32760 0.41 +0.8 2,745,979 + RR42_RS32760 0.42 -0.4 2,745,979 + RR42_RS32760 0.42 -0.1 2,745,979 + RR42_RS32760 0.42 -0.4 2,745,979 + RR42_RS32760 0.42 -1.4 2,745,980 - RR42_RS32760 0.42 +2.1 2,745,983 - RR42_RS32760 0.42 -0.3 2,746,021 + RR42_RS32760 0.45 +4.1 2,746,022 - RR42_RS32760 0.45 -1.3 2,746,022 - RR42_RS32760 0.45 -1.9 2,746,051 + RR42_RS32760 0.48 +0.9 2,746,052 - RR42_RS32760 0.48 -0.4 2,746,141 + RR42_RS32760 0.56 -1.4 2,746,142 - RR42_RS32760 0.56 +0.6 2,746,142 - RR42_RS32760 0.56 -1.3 2,746,142 - RR42_RS32760 0.56 -1.1 2,746,381 - RR42_RS32760 0.77 +2.4 2,746,809 - RR42_RS32765 0.18 -2.4 2,747,059 - RR42_RS32765 0.68 +0.5 2,747,059 - RR42_RS32765 0.68 -0.0 2,747,059 - RR42_RS32765 0.68 -1.9 2,747,267 + -2.1 2,747,314 + +0.4 2,747,317 + -1.1 2,747,317 + -2.6 2,747,318 - -0.7 2,747,318 - -0.3 2,747,341 + RR42_RS32770 0.10 -0.4 2,747,407 + RR42_RS32770 0.37 -0.1 2,747,505 - RR42_RS32770 0.76 -0.3 2,747,663 + RR42_RS32775 0.21 +1.8 2,747,663 + RR42_RS32775 0.21 +1.6 2,747,664 - RR42_RS32775 0.21 -0.2 2,747,664 - RR42_RS32775 0.21 -1.1 2,747,796 + +0.7 2,747,797 - -0.4 2,747,812 - -0.2 2,747,812 - -0.6 2,747,812 - +2.4 2,747,896 - +0.4 2,747,899 + -1.0 2,747,900 - -0.0 2,747,900 - +2.4 2,747,929 + -0.7 2,747,930 - -1.3 2,747,930 - -2.7 2,747,992 - +0.7
Or see this region's nucleotide sequence