Strain Fitness in Cupriavidus basilensis FW507-4G11 around RR42_RS32645

Experiment: R2A with Lomefloxacin 0.001 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRR42_RS32635 and RR42_RS32640 are separated by 38 nucleotidesRR42_RS32640 and RR42_RS32645 are separated by 32 nucleotidesRR42_RS32645 and RR42_RS32650 are separated by 8 nucleotides RR42_RS32635: RR42_RS32635 - 4-oxalocrotonate tautomerase, at 2,719,552 to 2,719,743 _RS32635 RR42_RS32640: RR42_RS32640 - 4-oxalocrotonate decarboxylase, at 2,719,782 to 2,720,570 _RS32640 RR42_RS32645: RR42_RS32645 - 2-keto-4-pentenoate hydratase, at 2,720,603 to 2,721,385 _RS32645 RR42_RS32650: RR42_RS32650 - betaine-aldehyde dehydrogenase, at 2,721,394 to 2,722,848 _RS32650 Position (kb) 2720 2721 2722Strain fitness (log2 ratio) -2 -1 0 1 2at 2720.589 kb on + strandat 2721.265 kb on + strand, within RR42_RS32645at 2721.271 kb on - strand, within RR42_RS32645at 2721.371 kb on - strandat 2721.428 kb on + strandat 2721.428 kb on + strandat 2721.663 kb on + strand, within RR42_RS32650at 2721.830 kb on + strand, within RR42_RS32650at 2721.972 kb on - strand, within RR42_RS32650at 2721.972 kb on - strand, within RR42_RS32650at 2722.111 kb on + strand, within RR42_RS32650at 2722.149 kb on + strand, within RR42_RS32650at 2722.244 kb on + strand, within RR42_RS32650

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Per-strain Table

Position Strand Gene LocusTag Fraction R2A with Lomefloxacin 0.001 mM
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2,720,589 + +0.9
2,721,265 + RR42_RS32645 0.85 +2.1
2,721,271 - RR42_RS32645 0.85 -0.2
2,721,371 - -1.8
2,721,428 + +0.8
2,721,428 + +0.4
2,721,663 + RR42_RS32650 0.18 +0.9
2,721,830 + RR42_RS32650 0.30 -2.3
2,721,972 - RR42_RS32650 0.40 -1.8
2,721,972 - RR42_RS32650 0.40 -0.0
2,722,111 + RR42_RS32650 0.49 -0.2
2,722,149 + RR42_RS32650 0.52 +1.1
2,722,244 + RR42_RS32650 0.58 -2.0

Or see this region's nucleotide sequence