Strain Fitness in Cupriavidus basilensis FW507-4G11 around RR42_RS32400

Experiment: R2A with Lomefloxacin 0.001 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRR42_RS32395 and RR42_RS32400 are separated by 360 nucleotidesRR42_RS32400 and RR42_RS32405 are separated by 203 nucleotidesRR42_RS32405 and RR42_RS32410 overlap by 4 nucleotides RR42_RS32395: RR42_RS32395 - IclR family transcriptional regulator, at 2,662,992 to 2,663,720 _RS32395 RR42_RS32400: RR42_RS32400 - hypothetical protein, at 2,664,081 to 2,664,551 _RS32400 RR42_RS32405: RR42_RS32405 - anti-sigma factor, at 2,664,755 to 2,665,546 _RS32405 RR42_RS32410: RR42_RS32410 - RNA polymerase subunit sigma-24, at 2,665,543 to 2,666,091 _RS32410 Position (kb) 2664 2665Strain fitness (log2 ratio) -2 -1 0 1 2 3at 2663.149 kb on - strand, within RR42_RS32395at 2663.205 kb on + strand, within RR42_RS32395at 2663.335 kb on + strand, within RR42_RS32395at 2663.371 kb on + strand, within RR42_RS32395at 2663.371 kb on + strand, within RR42_RS32395at 2663.372 kb on - strand, within RR42_RS32395at 2663.372 kb on - strand, within RR42_RS32395at 2663.452 kb on + strand, within RR42_RS32395at 2663.452 kb on + strand, within RR42_RS32395at 2663.652 kb on + strandat 2663.711 kb on + strandat 2663.711 kb on + strandat 2663.712 kb on - strandat 2663.712 kb on - strandat 2663.712 kb on - strandat 2664.249 kb on - strand, within RR42_RS32400at 2664.249 kb on - strand, within RR42_RS32400at 2664.286 kb on + strand, within RR42_RS32400at 2664.298 kb on + strand, within RR42_RS32400at 2664.298 kb on + strand, within RR42_RS32400at 2664.304 kb on + strand, within RR42_RS32400at 2664.319 kb on + strand, within RR42_RS32400at 2664.319 kb on + strand, within RR42_RS32400at 2664.341 kb on + strand, within RR42_RS32400at 2664.341 kb on + strand, within RR42_RS32400at 2664.342 kb on - strand, within RR42_RS32400at 2664.342 kb on - strand, within RR42_RS32400at 2664.342 kb on - strand, within RR42_RS32400at 2664.404 kb on - strand, within RR42_RS32400at 2664.451 kb on + strand, within RR42_RS32400at 2664.722 kb on + strandat 2664.756 kb on + strandat 2664.756 kb on + strandat 2664.756 kb on + strandat 2664.757 kb on - strandat 2664.757 kb on - strandat 2664.757 kb on - strandat 2664.873 kb on + strand, within RR42_RS32405at 2664.942 kb on + strand, within RR42_RS32405at 2664.942 kb on + strand, within RR42_RS32405at 2664.942 kb on + strand, within RR42_RS32405at 2664.943 kb on - strand, within RR42_RS32405at 2664.943 kb on - strand, within RR42_RS32405at 2664.975 kb on + strand, within RR42_RS32405at 2664.976 kb on - strand, within RR42_RS32405at 2664.976 kb on - strand, within RR42_RS32405at 2665.164 kb on + strand, within RR42_RS32405at 2665.164 kb on + strand, within RR42_RS32405at 2665.164 kb on + strand, within RR42_RS32405at 2665.165 kb on - strand, within RR42_RS32405at 2665.165 kb on - strand, within RR42_RS32405at 2665.165 kb on - strand, within RR42_RS32405at 2665.165 kb on - strand, within RR42_RS32405

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Per-strain Table

Position Strand Gene LocusTag Fraction R2A with Lomefloxacin 0.001 mM
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2,663,149 - RR42_RS32395 0.22 +0.2
2,663,205 + RR42_RS32395 0.29 -1.7
2,663,335 + RR42_RS32395 0.47 -0.3
2,663,371 + RR42_RS32395 0.52 -1.1
2,663,371 + RR42_RS32395 0.52 -1.8
2,663,372 - RR42_RS32395 0.52 +1.7
2,663,372 - RR42_RS32395 0.52 -0.3
2,663,452 + RR42_RS32395 0.63 -0.2
2,663,452 + RR42_RS32395 0.63 +1.5
2,663,652 + +0.4
2,663,711 + +0.9
2,663,711 + -1.6
2,663,712 - -1.0
2,663,712 - -0.0
2,663,712 - -0.2
2,664,249 - RR42_RS32400 0.36 -0.3
2,664,249 - RR42_RS32400 0.36 -2.6
2,664,286 + RR42_RS32400 0.44 +1.4
2,664,298 + RR42_RS32400 0.46 +3.4
2,664,298 + RR42_RS32400 0.46 +2.0
2,664,304 + RR42_RS32400 0.47 -1.0
2,664,319 + RR42_RS32400 0.51 -1.5
2,664,319 + RR42_RS32400 0.51 +2.5
2,664,341 + RR42_RS32400 0.55 +0.3
2,664,341 + RR42_RS32400 0.55 +0.8
2,664,342 - RR42_RS32400 0.55 +0.9
2,664,342 - RR42_RS32400 0.55 -0.5
2,664,342 - RR42_RS32400 0.55 +1.7
2,664,404 - RR42_RS32400 0.69 -0.3
2,664,451 + RR42_RS32400 0.79 -0.8
2,664,722 + -0.2
2,664,756 + +1.7
2,664,756 + +1.3
2,664,756 + +0.2
2,664,757 - -0.4
2,664,757 - -0.3
2,664,757 - +0.7
2,664,873 + RR42_RS32405 0.15 -0.5
2,664,942 + RR42_RS32405 0.24 -0.8
2,664,942 + RR42_RS32405 0.24 +0.7
2,664,942 + RR42_RS32405 0.24 -0.8
2,664,943 - RR42_RS32405 0.24 -1.1
2,664,943 - RR42_RS32405 0.24 -1.0
2,664,975 + RR42_RS32405 0.28 -1.7
2,664,976 - RR42_RS32405 0.28 +2.3
2,664,976 - RR42_RS32405 0.28 +0.7
2,665,164 + RR42_RS32405 0.52 -0.1
2,665,164 + RR42_RS32405 0.52 +2.1
2,665,164 + RR42_RS32405 0.52 +1.3
2,665,165 - RR42_RS32405 0.52 -0.3
2,665,165 - RR42_RS32405 0.52 -0.3
2,665,165 - RR42_RS32405 0.52 +1.0
2,665,165 - RR42_RS32405 0.52 +1.5

Or see this region's nucleotide sequence