Experiment: R2A with Lomefloxacin 0.001 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS31270 and RR42_RS31275 are separated by 105 nucleotides RR42_RS31275 and RR42_RS31280 are separated by 16 nucleotides RR42_RS31280 and RR42_RS31285 are separated by 528 nucleotides
RR42_RS31270: RR42_RS31270 - fumarylacetoacetase, at 2,390,093 to 2,391,355
_RS31270
RR42_RS31275: RR42_RS31275 - Homogentisate 1,2-dioxygenase (EC 1.13.11.5) (from data) , at 2,391,461 to 2,392,810
_RS31275
RR42_RS31280: RR42_RS31280 - hypothetical protein, at 2,392,827 to 2,393,054
_RS31280
RR42_RS31285: RR42_RS31285 - XRE family transcriptional regulator, at 2,393,583 to 2,393,816
_RS31285
RR42_RS31290: RR42_RS31290 - XRE family transcriptional regulator, at 2,393,721 to 2,393,975
_RS31290
Position (kb)
2391
2392
2393 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4
5 at 2390.461 kb on - strand, within RR42_RS31270 at 2390.461 kb on - strand, within RR42_RS31270 at 2390.461 kb on - strand, within RR42_RS31270 at 2390.461 kb on - strand, within RR42_RS31270 at 2390.613 kb on + strand, within RR42_RS31270 at 2390.613 kb on + strand, within RR42_RS31270 at 2390.614 kb on - strand, within RR42_RS31270 at 2390.752 kb on - strand, within RR42_RS31270 at 2390.752 kb on - strand, within RR42_RS31270 at 2391.242 kb on + strand at 2391.280 kb on - strand at 2391.280 kb on - strand at 2391.510 kb on + strand at 2391.510 kb on + strand at 2391.511 kb on - strand at 2391.511 kb on - strand at 2391.513 kb on + strand at 2391.514 kb on - strand at 2391.547 kb on - strand at 2391.547 kb on - strand at 2391.622 kb on + strand, within RR42_RS31275 at 2391.729 kb on + strand, within RR42_RS31275 at 2391.730 kb on - strand, within RR42_RS31275 at 2391.928 kb on + strand, within RR42_RS31275 at 2391.929 kb on - strand, within RR42_RS31275 at 2391.954 kb on + strand, within RR42_RS31275 at 2391.963 kb on + strand, within RR42_RS31275 at 2391.964 kb on - strand, within RR42_RS31275 at 2392.071 kb on + strand, within RR42_RS31275 at 2392.071 kb on + strand, within RR42_RS31275 at 2392.072 kb on - strand, within RR42_RS31275 at 2392.072 kb on - strand, within RR42_RS31275 at 2392.072 kb on - strand, within RR42_RS31275 at 2392.161 kb on + strand, within RR42_RS31275 at 2392.161 kb on + strand, within RR42_RS31275 at 2392.162 kb on - strand, within RR42_RS31275 at 2392.174 kb on + strand, within RR42_RS31275 at 2392.175 kb on - strand, within RR42_RS31275 at 2392.175 kb on - strand, within RR42_RS31275 at 2392.175 kb on - strand, within RR42_RS31275 at 2392.176 kb on + strand, within RR42_RS31275 at 2392.369 kb on - strand, within RR42_RS31275 at 2392.489 kb on + strand, within RR42_RS31275 at 2392.647 kb on + strand, within RR42_RS31275 at 2392.647 kb on + strand, within RR42_RS31275 at 2392.648 kb on - strand, within RR42_RS31275 at 2392.657 kb on - strand, within RR42_RS31275 at 2392.744 kb on - strand at 2392.828 kb on + strand at 2392.828 kb on + strand at 2392.829 kb on - strand at 2392.829 kb on - strand at 2392.829 kb on - strand at 2393.008 kb on + strand, within RR42_RS31280 at 2393.008 kb on + strand, within RR42_RS31280 at 2393.008 kb on + strand, within RR42_RS31280 at 2393.009 kb on - strand, within RR42_RS31280 at 2393.009 kb on - strand, within RR42_RS31280 at 2393.009 kb on - strand, within RR42_RS31280 at 2393.043 kb on - strand at 2393.179 kb on + strand at 2393.209 kb on + strand at 2393.210 kb on - strand at 2393.219 kb on + strand at 2393.253 kb on + strand at 2393.253 kb on + strand at 2393.253 kb on + strand at 2393.253 kb on + strand at 2393.254 kb on - strand at 2393.254 kb on - strand at 2393.261 kb on + strand at 2393.261 kb on + strand at 2393.262 kb on - strand at 2393.262 kb on - strand at 2393.262 kb on - strand at 2393.262 kb on - strand at 2393.262 kb on - strand at 2393.262 kb on - strand at 2393.262 kb on - strand at 2393.262 kb on - strand at 2393.656 kb on - strand, within RR42_RS31285 at 2393.730 kb on - strand at 2393.730 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Lomefloxacin 0.001 mM remove 2,390,461 - RR42_RS31270 0.29 +1.0 2,390,461 - RR42_RS31270 0.29 +0.3 2,390,461 - RR42_RS31270 0.29 +0.1 2,390,461 - RR42_RS31270 0.29 +1.1 2,390,613 + RR42_RS31270 0.41 +0.8 2,390,613 + RR42_RS31270 0.41 +1.3 2,390,614 - RR42_RS31270 0.41 +1.8 2,390,752 - RR42_RS31270 0.52 -1.8 2,390,752 - RR42_RS31270 0.52 +0.3 2,391,242 + +2.0 2,391,280 - +2.2 2,391,280 - +2.5 2,391,510 + +1.9 2,391,510 + +0.1 2,391,511 - -1.2 2,391,511 - -0.8 2,391,513 + +1.1 2,391,514 - -1.1 2,391,547 - -0.7 2,391,547 - +2.1 2,391,622 + RR42_RS31275 0.12 -0.6 2,391,729 + RR42_RS31275 0.20 -0.2 2,391,730 - RR42_RS31275 0.20 +0.6 2,391,928 + RR42_RS31275 0.35 -0.6 2,391,929 - RR42_RS31275 0.35 -0.6 2,391,954 + RR42_RS31275 0.37 -0.9 2,391,963 + RR42_RS31275 0.37 -1.3 2,391,964 - RR42_RS31275 0.37 +0.6 2,392,071 + RR42_RS31275 0.45 +0.1 2,392,071 + RR42_RS31275 0.45 +1.4 2,392,072 - RR42_RS31275 0.45 -0.3 2,392,072 - RR42_RS31275 0.45 +0.6 2,392,072 - RR42_RS31275 0.45 -1.4 2,392,161 + RR42_RS31275 0.52 -2.2 2,392,161 + RR42_RS31275 0.52 -0.1 2,392,162 - RR42_RS31275 0.52 -0.1 2,392,174 + RR42_RS31275 0.53 +0.4 2,392,175 - RR42_RS31275 0.53 +1.4 2,392,175 - RR42_RS31275 0.53 +1.9 2,392,175 - RR42_RS31275 0.53 -0.4 2,392,176 + RR42_RS31275 0.53 -0.4 2,392,369 - RR42_RS31275 0.67 -2.3 2,392,489 + RR42_RS31275 0.76 -1.8 2,392,647 + RR42_RS31275 0.88 +1.2 2,392,647 + RR42_RS31275 0.88 -0.5 2,392,648 - RR42_RS31275 0.88 +1.9 2,392,657 - RR42_RS31275 0.89 +0.9 2,392,744 - +0.5 2,392,828 + +0.6 2,392,828 + +0.8 2,392,829 - -0.5 2,392,829 - +0.4 2,392,829 - +1.0 2,393,008 + RR42_RS31280 0.79 +0.6 2,393,008 + RR42_RS31280 0.79 +1.4 2,393,008 + RR42_RS31280 0.79 +0.9 2,393,009 - RR42_RS31280 0.80 +0.2 2,393,009 - RR42_RS31280 0.80 -1.2 2,393,009 - RR42_RS31280 0.80 +0.4 2,393,043 - -0.8 2,393,179 + +0.9 2,393,209 + +0.5 2,393,210 - -1.6 2,393,219 + -1.5 2,393,253 + +1.9 2,393,253 + +0.1 2,393,253 + -1.1 2,393,253 + +0.8 2,393,254 - -0.5 2,393,254 - -0.7 2,393,261 + +1.4 2,393,261 + +0.1 2,393,262 - +0.2 2,393,262 - -0.2 2,393,262 - -1.6 2,393,262 - +0.3 2,393,262 - +0.4 2,393,262 - +1.2 2,393,262 - +0.1 2,393,262 - +0.9 2,393,656 - RR42_RS31285 0.31 +2.8 2,393,730 - +5.5 2,393,730 - +4.2
Or see this region's nucleotide sequence