Experiment: R2A with Lomefloxacin 0.001 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS30040 and RR42_RS30045 overlap by 14 nucleotides RR42_RS30045 and RR42_RS30050 are separated by 118 nucleotides RR42_RS30050 and RR42_RS30055 are separated by 91 nucleotides
RR42_RS30040: RR42_RS30040 - fusaric acid resistance protein, at 2,111,240 to 2,113,264
_RS30040
RR42_RS30045: RR42_RS30045 - RND transporter, at 2,113,251 to 2,114,789
_RS30045
RR42_RS30050: RR42_RS30050 - hypothetical protein, at 2,114,908 to 2,115,321
_RS30050
RR42_RS30055: RR42_RS30055 - hypothetical protein, at 2,115,413 to 2,115,685
_RS30055
Position (kb)
2113
2114
2115 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 2112.299 kb on - strand, within RR42_RS30040 at 2112.299 kb on - strand, within RR42_RS30040 at 2112.357 kb on - strand, within RR42_RS30040 at 2112.366 kb on - strand, within RR42_RS30040 at 2112.394 kb on + strand, within RR42_RS30040 at 2112.394 kb on + strand, within RR42_RS30040 at 2112.394 kb on + strand, within RR42_RS30040 at 2112.394 kb on + strand, within RR42_RS30040 at 2112.395 kb on - strand, within RR42_RS30040 at 2112.395 kb on - strand, within RR42_RS30040 at 2112.395 kb on - strand, within RR42_RS30040 at 2112.395 kb on - strand, within RR42_RS30040 at 2112.413 kb on + strand, within RR42_RS30040 at 2112.414 kb on - strand, within RR42_RS30040 at 2112.426 kb on + strand, within RR42_RS30040 at 2112.515 kb on + strand, within RR42_RS30040 at 2112.515 kb on + strand, within RR42_RS30040 at 2112.515 kb on + strand, within RR42_RS30040 at 2112.516 kb on - strand, within RR42_RS30040 at 2112.516 kb on - strand, within RR42_RS30040 at 2112.516 kb on - strand, within RR42_RS30040 at 2112.516 kb on - strand, within RR42_RS30040 at 2112.516 kb on - strand, within RR42_RS30040 at 2112.516 kb on - strand, within RR42_RS30040 at 2112.516 kb on - strand, within RR42_RS30040 at 2112.516 kb on - strand, within RR42_RS30040 at 2112.597 kb on + strand, within RR42_RS30040 at 2112.617 kb on + strand, within RR42_RS30040 at 2112.647 kb on + strand, within RR42_RS30040 at 2112.648 kb on - strand, within RR42_RS30040 at 2112.648 kb on - strand, within RR42_RS30040 at 2112.648 kb on - strand, within RR42_RS30040 at 2112.741 kb on - strand, within RR42_RS30040 at 2112.741 kb on - strand, within RR42_RS30040 at 2113.242 kb on - strand at 2113.510 kb on - strand, within RR42_RS30045 at 2113.620 kb on + strand, within RR42_RS30045 at 2113.620 kb on + strand, within RR42_RS30045 at 2113.821 kb on - strand, within RR42_RS30045 at 2113.876 kb on - strand, within RR42_RS30045 at 2113.876 kb on - strand, within RR42_RS30045 at 2114.070 kb on + strand, within RR42_RS30045 at 2114.097 kb on - strand, within RR42_RS30045 at 2114.097 kb on - strand, within RR42_RS30045 at 2114.310 kb on + strand, within RR42_RS30045 at 2114.310 kb on + strand, within RR42_RS30045 at 2114.310 kb on + strand, within RR42_RS30045 at 2114.311 kb on - strand, within RR42_RS30045 at 2114.386 kb on - strand, within RR42_RS30045 at 2114.404 kb on + strand, within RR42_RS30045 at 2114.431 kb on - strand, within RR42_RS30045 at 2114.431 kb on - strand, within RR42_RS30045 at 2114.431 kb on - strand, within RR42_RS30045 at 2114.574 kb on + strand, within RR42_RS30045 at 2114.616 kb on + strand, within RR42_RS30045 at 2114.616 kb on + strand, within RR42_RS30045 at 2114.617 kb on - strand, within RR42_RS30045 at 2114.617 kb on - strand, within RR42_RS30045 at 2114.639 kb on + strand at 2114.640 kb on - strand at 2114.640 kb on - strand at 2114.640 kb on - strand at 2114.640 kb on - strand at 2114.640 kb on - strand at 2114.640 kb on - strand at 2114.640 kb on - strand at 2114.662 kb on + strand at 2114.662 kb on + strand at 2114.663 kb on - strand at 2114.663 kb on - strand at 2114.663 kb on - strand at 2114.663 kb on - strand at 2114.722 kb on - strand at 2114.722 kb on - strand at 2114.933 kb on - strand at 2114.933 kb on - strand at 2114.941 kb on - strand at 2115.362 kb on + strand at 2115.362 kb on + strand at 2115.363 kb on - strand at 2115.437 kb on + strand at 2115.438 kb on - strand at 2115.545 kb on - strand, within RR42_RS30055 at 2115.564 kb on + strand, within RR42_RS30055 at 2115.564 kb on + strand, within RR42_RS30055 at 2115.564 kb on + strand, within RR42_RS30055 at 2115.564 kb on + strand, within RR42_RS30055 at 2115.565 kb on - strand, within RR42_RS30055 at 2115.708 kb on + strand at 2115.709 kb on - strand at 2115.709 kb on - strand at 2115.709 kb on - strand at 2115.788 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Lomefloxacin 0.001 mM remove 2,112,299 - RR42_RS30040 0.52 +0.8 2,112,299 - RR42_RS30040 0.52 -1.7 2,112,357 - RR42_RS30040 0.55 -0.4 2,112,366 - RR42_RS30040 0.56 +1.0 2,112,394 + RR42_RS30040 0.57 -0.7 2,112,394 + RR42_RS30040 0.57 -1.5 2,112,394 + RR42_RS30040 0.57 +2.0 2,112,394 + RR42_RS30040 0.57 -0.2 2,112,395 - RR42_RS30040 0.57 -0.6 2,112,395 - RR42_RS30040 0.57 +0.7 2,112,395 - RR42_RS30040 0.57 -4.1 2,112,395 - RR42_RS30040 0.57 -0.2 2,112,413 + RR42_RS30040 0.58 -0.7 2,112,414 - RR42_RS30040 0.58 -0.7 2,112,426 + RR42_RS30040 0.59 +2.1 2,112,515 + RR42_RS30040 0.63 -0.6 2,112,515 + RR42_RS30040 0.63 -0.6 2,112,515 + RR42_RS30040 0.63 -2.5 2,112,516 - RR42_RS30040 0.63 -2.0 2,112,516 - RR42_RS30040 0.63 +0.3 2,112,516 - RR42_RS30040 0.63 -1.5 2,112,516 - RR42_RS30040 0.63 -0.1 2,112,516 - RR42_RS30040 0.63 -0.6 2,112,516 - RR42_RS30040 0.63 -1.7 2,112,516 - RR42_RS30040 0.63 -0.3 2,112,516 - RR42_RS30040 0.63 -0.7 2,112,597 + RR42_RS30040 0.67 -0.3 2,112,617 + RR42_RS30040 0.68 +0.0 2,112,647 + RR42_RS30040 0.69 +0.6 2,112,648 - RR42_RS30040 0.70 +0.8 2,112,648 - RR42_RS30040 0.70 -0.1 2,112,648 - RR42_RS30040 0.70 +0.1 2,112,741 - RR42_RS30040 0.74 -0.7 2,112,741 - RR42_RS30040 0.74 -1.0 2,113,242 - -0.9 2,113,510 - RR42_RS30045 0.17 -1.0 2,113,620 + RR42_RS30045 0.24 -0.1 2,113,620 + RR42_RS30045 0.24 -0.6 2,113,821 - RR42_RS30045 0.37 +0.4 2,113,876 - RR42_RS30045 0.41 +0.8 2,113,876 - RR42_RS30045 0.41 +0.3 2,114,070 + RR42_RS30045 0.53 +0.1 2,114,097 - RR42_RS30045 0.55 +0.7 2,114,097 - RR42_RS30045 0.55 -1.0 2,114,310 + RR42_RS30045 0.69 -1.5 2,114,310 + RR42_RS30045 0.69 +0.2 2,114,310 + RR42_RS30045 0.69 +0.4 2,114,311 - RR42_RS30045 0.69 -0.9 2,114,386 - RR42_RS30045 0.74 +0.2 2,114,404 + RR42_RS30045 0.75 +2.1 2,114,431 - RR42_RS30045 0.77 -0.6 2,114,431 - RR42_RS30045 0.77 -0.6 2,114,431 - RR42_RS30045 0.77 -2.5 2,114,574 + RR42_RS30045 0.86 +0.3 2,114,616 + RR42_RS30045 0.89 -0.6 2,114,616 + RR42_RS30045 0.89 -1.4 2,114,617 - RR42_RS30045 0.89 -0.1 2,114,617 - RR42_RS30045 0.89 -0.2 2,114,639 + +0.2 2,114,640 - -0.6 2,114,640 - -0.5 2,114,640 - +0.0 2,114,640 - +1.8 2,114,640 - -1.4 2,114,640 - -0.3 2,114,640 - +0.4 2,114,662 + -1.0 2,114,662 + -1.6 2,114,663 - +0.8 2,114,663 - +0.5 2,114,663 - +1.3 2,114,663 - -1.8 2,114,722 - +0.5 2,114,722 - +1.1 2,114,933 - -0.1 2,114,933 - -1.2 2,114,941 - +0.2 2,115,362 + -0.6 2,115,362 + -1.0 2,115,363 - -0.0 2,115,437 + +0.1 2,115,438 - +0.2 2,115,545 - RR42_RS30055 0.48 +0.4 2,115,564 + RR42_RS30055 0.55 +2.9 2,115,564 + RR42_RS30055 0.55 -1.2 2,115,564 + RR42_RS30055 0.55 -1.2 2,115,564 + RR42_RS30055 0.55 +0.2 2,115,565 - RR42_RS30055 0.56 -0.4 2,115,708 + -0.0 2,115,709 - -1.4 2,115,709 - +0.2 2,115,709 - +0.9 2,115,788 + +1.1
Or see this region's nucleotide sequence