Experiment: R2A with Lomefloxacin 0.001 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS29485 and RR42_RS29490 are separated by 264 nucleotides RR42_RS29490 and RR42_RS29495 are separated by 2 nucleotides RR42_RS29495 and RR42_RS29500 are separated by 153 nucleotides
RR42_RS29485: RR42_RS29485 - fatty-acid--CoA ligase, at 1,971,968 to 1,973,593
_RS29485
RR42_RS29490: RR42_RS29490 - toxin HipA, at 1,973,858 to 1,974,109
_RS29490
RR42_RS29495: RR42_RS29495 - toxin HipA, at 1,974,112 to 1,975,452
_RS29495
RR42_RS29500: RR42_RS29500 - acyl-CoA dehydrogenase, at 1,975,606 to 1,976,781
_RS29500
Position (kb)
1974
1975
1976 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1973.296 kb on + strand, within RR42_RS29485 at 1973.296 kb on + strand, within RR42_RS29485 at 1973.296 kb on + strand, within RR42_RS29485 at 1973.296 kb on + strand, within RR42_RS29485 at 1973.297 kb on - strand, within RR42_RS29485 at 1973.297 kb on - strand, within RR42_RS29485 at 1973.297 kb on - strand, within RR42_RS29485 at 1973.297 kb on - strand, within RR42_RS29485 at 1973.297 kb on - strand, within RR42_RS29485 at 1973.297 kb on - strand, within RR42_RS29485 at 1973.297 kb on - strand, within RR42_RS29485 at 1973.297 kb on - strand, within RR42_RS29485 at 1973.297 kb on - strand, within RR42_RS29485 at 1973.515 kb on - strand at 1973.664 kb on + strand at 1973.664 kb on + strand at 1973.665 kb on - strand at 1973.672 kb on + strand at 1973.672 kb on + strand at 1973.672 kb on + strand at 1973.673 kb on - strand at 1973.763 kb on - strand at 1973.763 kb on - strand at 1973.805 kb on + strand at 1973.984 kb on - strand, within RR42_RS29490 at 1974.068 kb on - strand, within RR42_RS29490 at 1974.107 kb on + strand at 1974.108 kb on - strand at 1974.146 kb on + strand at 1974.168 kb on - strand at 1974.212 kb on - strand at 1974.279 kb on + strand, within RR42_RS29495 at 1974.279 kb on + strand, within RR42_RS29495 at 1974.280 kb on - strand, within RR42_RS29495 at 1974.313 kb on + strand, within RR42_RS29495 at 1974.723 kb on + strand, within RR42_RS29495 at 1974.724 kb on - strand, within RR42_RS29495 at 1974.724 kb on - strand, within RR42_RS29495 at 1974.757 kb on - strand, within RR42_RS29495 at 1974.769 kb on + strand, within RR42_RS29495 at 1974.770 kb on - strand, within RR42_RS29495 at 1974.790 kb on - strand, within RR42_RS29495 at 1974.931 kb on + strand, within RR42_RS29495 at 1974.932 kb on - strand, within RR42_RS29495 at 1974.932 kb on - strand, within RR42_RS29495 at 1975.123 kb on + strand, within RR42_RS29495 at 1975.124 kb on - strand, within RR42_RS29495 at 1975.124 kb on - strand, within RR42_RS29495 at 1975.124 kb on - strand, within RR42_RS29495 at 1975.226 kb on - strand, within RR42_RS29495 at 1975.226 kb on - strand, within RR42_RS29495 at 1975.451 kb on - strand at 1975.451 kb on - strand at 1975.458 kb on + strand at 1975.458 kb on + strand at 1975.459 kb on - strand at 1975.459 kb on - strand at 1975.459 kb on - strand at 1975.523 kb on + strand at 1975.523 kb on + strand at 1975.524 kb on - strand at 1975.639 kb on + strand at 1975.639 kb on + strand at 1975.640 kb on - strand at 1975.708 kb on + strand at 1975.708 kb on + strand at 1975.708 kb on + strand at 1975.708 kb on + strand at 1975.708 kb on + strand at 1975.709 kb on - strand at 1975.709 kb on - strand at 1975.753 kb on + strand, within RR42_RS29500 at 1975.754 kb on - strand, within RR42_RS29500 at 1975.831 kb on + strand, within RR42_RS29500 at 1975.831 kb on + strand, within RR42_RS29500 at 1975.831 kb on + strand, within RR42_RS29500 at 1975.832 kb on - strand, within RR42_RS29500 at 1975.832 kb on - strand, within RR42_RS29500 at 1975.832 kb on - strand, within RR42_RS29500 at 1976.071 kb on + strand, within RR42_RS29500 at 1976.071 kb on + strand, within RR42_RS29500 at 1976.071 kb on + strand, within RR42_RS29500 at 1976.071 kb on + strand, within RR42_RS29500 at 1976.072 kb on - strand, within RR42_RS29500 at 1976.072 kb on - strand, within RR42_RS29500 at 1976.380 kb on + strand, within RR42_RS29500 at 1976.380 kb on + strand, within RR42_RS29500 at 1976.381 kb on - strand, within RR42_RS29500 at 1976.381 kb on - strand, within RR42_RS29500 at 1976.386 kb on + strand, within RR42_RS29500 at 1976.386 kb on + strand, within RR42_RS29500 at 1976.387 kb on - strand, within RR42_RS29500 at 1976.387 kb on - strand, within RR42_RS29500
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Lomefloxacin 0.001 mM remove 1,973,296 + RR42_RS29485 0.82 -0.8 1,973,296 + RR42_RS29485 0.82 -2.6 1,973,296 + RR42_RS29485 0.82 -1.7 1,973,296 + RR42_RS29485 0.82 +0.1 1,973,297 - RR42_RS29485 0.82 +0.7 1,973,297 - RR42_RS29485 0.82 +0.1 1,973,297 - RR42_RS29485 0.82 -1.7 1,973,297 - RR42_RS29485 0.82 -1.0 1,973,297 - RR42_RS29485 0.82 +1.9 1,973,297 - RR42_RS29485 0.82 -1.9 1,973,297 - RR42_RS29485 0.82 +2.1 1,973,297 - RR42_RS29485 0.82 -0.6 1,973,297 - RR42_RS29485 0.82 +1.2 1,973,515 - -1.4 1,973,664 + -1.5 1,973,664 + +0.4 1,973,665 - +1.2 1,973,672 + -1.0 1,973,672 + -0.3 1,973,672 + +0.0 1,973,673 - +0.7 1,973,763 - +0.9 1,973,763 - -2.2 1,973,805 + +0.7 1,973,984 - RR42_RS29490 0.50 -0.4 1,974,068 - RR42_RS29490 0.83 -1.2 1,974,107 + -0.9 1,974,108 - -2.3 1,974,146 + +0.4 1,974,168 - +1.7 1,974,212 - -1.8 1,974,279 + RR42_RS29495 0.12 -0.2 1,974,279 + RR42_RS29495 0.12 -0.1 1,974,280 - RR42_RS29495 0.13 +1.1 1,974,313 + RR42_RS29495 0.15 -0.3 1,974,723 + RR42_RS29495 0.46 -1.4 1,974,724 - RR42_RS29495 0.46 -1.4 1,974,724 - RR42_RS29495 0.46 -0.3 1,974,757 - RR42_RS29495 0.48 -1.0 1,974,769 + RR42_RS29495 0.49 -0.9 1,974,770 - RR42_RS29495 0.49 +0.6 1,974,790 - RR42_RS29495 0.51 -0.9 1,974,931 + RR42_RS29495 0.61 -0.6 1,974,932 - RR42_RS29495 0.61 -1.2 1,974,932 - RR42_RS29495 0.61 +0.8 1,975,123 + RR42_RS29495 0.75 +0.7 1,975,124 - RR42_RS29495 0.75 -1.3 1,975,124 - RR42_RS29495 0.75 +0.7 1,975,124 - RR42_RS29495 0.75 -0.9 1,975,226 - RR42_RS29495 0.83 -1.1 1,975,226 - RR42_RS29495 0.83 -2.2 1,975,451 - -1.4 1,975,451 - -0.5 1,975,458 + -0.4 1,975,458 + +2.2 1,975,459 - -0.9 1,975,459 - -0.3 1,975,459 - -1.4 1,975,523 + -1.4 1,975,523 + +0.7 1,975,524 - -0.0 1,975,639 + -1.3 1,975,639 + +1.1 1,975,640 - -2.4 1,975,708 + -0.7 1,975,708 + -1.4 1,975,708 + -0.9 1,975,708 + +0.6 1,975,708 + +0.7 1,975,709 - -0.0 1,975,709 - -0.8 1,975,753 + RR42_RS29500 0.12 -1.2 1,975,754 - RR42_RS29500 0.13 +1.4 1,975,831 + RR42_RS29500 0.19 +0.5 1,975,831 + RR42_RS29500 0.19 +0.5 1,975,831 + RR42_RS29500 0.19 +0.1 1,975,832 - RR42_RS29500 0.19 +0.7 1,975,832 - RR42_RS29500 0.19 -0.9 1,975,832 - RR42_RS29500 0.19 -1.3 1,976,071 + RR42_RS29500 0.40 -1.1 1,976,071 + RR42_RS29500 0.40 -0.7 1,976,071 + RR42_RS29500 0.40 +0.7 1,976,071 + RR42_RS29500 0.40 -0.8 1,976,072 - RR42_RS29500 0.40 +0.2 1,976,072 - RR42_RS29500 0.40 +0.2 1,976,380 + RR42_RS29500 0.66 -1.9 1,976,380 + RR42_RS29500 0.66 -1.8 1,976,381 - RR42_RS29500 0.66 -1.2 1,976,381 - RR42_RS29500 0.66 -0.3 1,976,386 + RR42_RS29500 0.66 +0.3 1,976,386 + RR42_RS29500 0.66 -1.0 1,976,387 - RR42_RS29500 0.66 +0.5 1,976,387 - RR42_RS29500 0.66 +0.2
Or see this region's nucleotide sequence