Strain Fitness in Cupriavidus basilensis FW507-4G11 around RR42_RS29250

Experiment: R2A with Lomefloxacin 0.001 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRR42_RS29240 and RR42_RS29245 overlap by 4 nucleotidesRR42_RS29245 and RR42_RS29250 overlap by 8 nucleotidesRR42_RS29250 and RR42_RS29255 are separated by 174 nucleotidesRR42_RS29255 and RR42_RS29260 are separated by 129 nucleotides RR42_RS29240: RR42_RS29240 - nitrile hydratase subunit alpha, at 1,914,971 to 1,915,675 _RS29240 RR42_RS29245: RR42_RS29245 - nitrile hydratase, at 1,915,672 to 1,916,343 _RS29245 RR42_RS29250: RR42_RS29250 - nitrile hydratase, at 1,916,336 to 1,916,722 _RS29250 RR42_RS29255: RR42_RS29255 - GTP cyclohydrolase, at 1,916,897 to 1,917,493 _RS29255 RR42_RS29260: RR42_RS29260 - hypothetical protein, at 1,917,623 to 1,918,972 _RS29260 Position (kb) 1916 1917Strain fitness (log2 ratio) -2 -1 0 1 2at 1915.374 kb on - strand, within RR42_RS29240at 1915.374 kb on - strand, within RR42_RS29240at 1915.409 kb on + strand, within RR42_RS29240at 1915.409 kb on + strand, within RR42_RS29240at 1915.410 kb on - strand, within RR42_RS29240at 1915.410 kb on - strand, within RR42_RS29240at 1915.532 kb on + strand, within RR42_RS29240at 1915.532 kb on + strand, within RR42_RS29240at 1915.533 kb on - strand, within RR42_RS29240at 1915.533 kb on - strand, within RR42_RS29240at 1915.533 kb on - strand, within RR42_RS29240at 1915.596 kb on + strand, within RR42_RS29240at 1915.596 kb on + strand, within RR42_RS29240at 1915.597 kb on - strand, within RR42_RS29240at 1915.742 kb on + strand, within RR42_RS29245at 1915.812 kb on + strand, within RR42_RS29245at 1915.858 kb on + strand, within RR42_RS29245at 1915.858 kb on + strand, within RR42_RS29245at 1915.858 kb on + strand, within RR42_RS29245at 1915.859 kb on - strand, within RR42_RS29245at 1915.859 kb on - strand, within RR42_RS29245at 1915.859 kb on - strand, within RR42_RS29245at 1915.859 kb on - strand, within RR42_RS29245at 1915.873 kb on + strand, within RR42_RS29245at 1915.876 kb on + strand, within RR42_RS29245at 1915.877 kb on - strand, within RR42_RS29245at 1916.131 kb on + strand, within RR42_RS29245at 1916.131 kb on + strand, within RR42_RS29245at 1916.132 kb on - strand, within RR42_RS29245at 1916.230 kb on + strandat 1916.230 kb on + strand, within RR42_RS29245at 1916.231 kb on - strand, within RR42_RS29245at 1916.311 kb on + strandat 1916.312 kb on - strandat 1916.312 kb on - strandat 1916.312 kb on - strandat 1916.579 kb on + strand, within RR42_RS29250at 1916.579 kb on + strand, within RR42_RS29250at 1916.579 kb on + strand, within RR42_RS29250at 1916.579 kb on + strand, within RR42_RS29250at 1916.580 kb on - strand, within RR42_RS29250at 1916.580 kb on - strand, within RR42_RS29250at 1916.580 kb on - strand, within RR42_RS29250at 1916.582 kb on + strand, within RR42_RS29250at 1916.583 kb on - strand, within RR42_RS29250at 1916.583 kb on - strand, within RR42_RS29250at 1916.744 kb on + strandat 1916.825 kb on - strandat 1916.837 kb on + strandat 1916.845 kb on - strandat 1916.960 kb on - strand, within RR42_RS29255at 1916.960 kb on - strand, within RR42_RS29255at 1917.164 kb on + strand, within RR42_RS29255at 1917.164 kb on + strand, within RR42_RS29255at 1917.218 kb on + strand, within RR42_RS29255at 1917.219 kb on - strand, within RR42_RS29255at 1917.219 kb on - strand, within RR42_RS29255at 1917.245 kb on - strand, within RR42_RS29255at 1917.293 kb on + strand, within RR42_RS29255at 1917.294 kb on - strand, within RR42_RS29255at 1917.294 kb on - strand, within RR42_RS29255at 1917.418 kb on + strand, within RR42_RS29255at 1917.418 kb on + strand, within RR42_RS29255at 1917.419 kb on - strand, within RR42_RS29255

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Per-strain Table

Position Strand Gene LocusTag Fraction R2A with Lomefloxacin 0.001 mM
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1,915,374 - RR42_RS29240 0.57 -1.8
1,915,374 - RR42_RS29240 0.57 -0.7
1,915,409 + RR42_RS29240 0.62 -0.6
1,915,409 + RR42_RS29240 0.62 -0.4
1,915,410 - RR42_RS29240 0.62 +0.4
1,915,410 - RR42_RS29240 0.62 -2.5
1,915,532 + RR42_RS29240 0.80 +1.5
1,915,532 + RR42_RS29240 0.80 -0.5
1,915,533 - RR42_RS29240 0.80 -0.4
1,915,533 - RR42_RS29240 0.80 +1.1
1,915,533 - RR42_RS29240 0.80 -2.1
1,915,596 + RR42_RS29240 0.89 +0.7
1,915,596 + RR42_RS29240 0.89 +0.7
1,915,597 - RR42_RS29240 0.89 +0.6
1,915,742 + RR42_RS29245 0.10 +0.1
1,915,812 + RR42_RS29245 0.21 +0.4
1,915,858 + RR42_RS29245 0.28 +0.4
1,915,858 + RR42_RS29245 0.28 +1.9
1,915,858 + RR42_RS29245 0.28 +0.7
1,915,859 - RR42_RS29245 0.28 +0.5
1,915,859 - RR42_RS29245 0.28 -2.5
1,915,859 - RR42_RS29245 0.28 +1.3
1,915,859 - RR42_RS29245 0.28 -0.8
1,915,873 + RR42_RS29245 0.30 +0.5
1,915,876 + RR42_RS29245 0.30 +0.1
1,915,877 - RR42_RS29245 0.31 -1.9
1,916,131 + RR42_RS29245 0.68 -1.6
1,916,131 + RR42_RS29245 0.68 -0.3
1,916,132 - RR42_RS29245 0.68 +0.5
1,916,230 + +2.7
1,916,230 + RR42_RS29245 0.83 +1.0
1,916,231 - RR42_RS29245 0.83 +0.5
1,916,311 + -1.1
1,916,312 - -1.1
1,916,312 - -0.8
1,916,312 - -0.2
1,916,579 + RR42_RS29250 0.63 -0.5
1,916,579 + RR42_RS29250 0.63 +0.3
1,916,579 + RR42_RS29250 0.63 +0.4
1,916,579 + RR42_RS29250 0.63 -0.2
1,916,580 - RR42_RS29250 0.63 -0.3
1,916,580 - RR42_RS29250 0.63 +1.2
1,916,580 - RR42_RS29250 0.63 -0.3
1,916,582 + RR42_RS29250 0.64 -1.5
1,916,583 - RR42_RS29250 0.64 +0.2
1,916,583 - RR42_RS29250 0.64 -1.3
1,916,744 + -1.9
1,916,825 - +0.3
1,916,837 + -0.7
1,916,845 - -0.6
1,916,960 - RR42_RS29255 0.11 -1.1
1,916,960 - RR42_RS29255 0.11 -0.2
1,917,164 + RR42_RS29255 0.45 -1.5
1,917,164 + RR42_RS29255 0.45 -2.2
1,917,218 + RR42_RS29255 0.54 +2.0
1,917,219 - RR42_RS29255 0.54 -1.1
1,917,219 - RR42_RS29255 0.54 +2.7
1,917,245 - RR42_RS29255 0.58 -1.6
1,917,293 + RR42_RS29255 0.66 -0.3
1,917,294 - RR42_RS29255 0.66 -0.5
1,917,294 - RR42_RS29255 0.66 -1.8
1,917,418 + RR42_RS29255 0.87 -1.2
1,917,418 + RR42_RS29255 0.87 +0.8
1,917,419 - RR42_RS29255 0.87 +2.4

Or see this region's nucleotide sequence